Numb Antibody


Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.
Immunocytochemistry/ Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against more
Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation more

Product Details

Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, Simple Western, ICC/IF
3.5 mg/ml

Numb Antibody Summary

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggest that mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different pro...cesses. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.
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A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
This is specific for all four isoforms of the NUMB protein.
Predicted Species
Rat (90%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
3.5 mg/ml
Immunogen affinity purified


Application Notes
This NUMB antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
Numb Peptide (NB500-178PEP)

Reactivity Notes

Human, mouse and chicken. Immunogen sequence has 90% identity to rat.

Alternate Names for Numb Antibody

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • protein numb homolog
  • Protein S171
  • S171


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Numb Antibody (NB500-178): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Numb Antibody (NB500-178). (Showing 1 - 2 of 2 FAQ).

  1. I have looked at the data sheet for NB500-178 and do not see any reference to IP, but for some reason it was listed on our data sheet. Do you by chance have any reports of attempted use of the antibody in IP or any historical information regarding a listing for IP that might be helpful?
    • I pulled data on this target and I am not showing any notes of it being removed or ever being tested in that application. So unfortunately I have nothing to provide.
  2. I'm using your NUMB Antibody, cat# NB500-178. Does it pick up Numb Like protein also?
    • We performed an alignment of the immunogen sequence against the numb like protein for mouse based on UniProt. We show an approximately 50% similarity between the two sequences. As a result, we do not believe this antibody will recognize Numb-like protein. However, we have to stipulate that the lab has not tested the binding between the numb like protein and this antibody directly. Therefore, we cannot say with certainty whether the numb like protein will also be detected. Especially due to the fact that numb like protein has a similar molecular weight to one of the isoforms of the numb protein this antibody targets making it more difficult to analyze. Based on the sequence analysis information, it appears unlikely that the antibody detects numb like protein.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB500-178AF405
Alexa Fluor (R) 488 Labeled NB500-178AF488
Alexa Fluor (R) 647 Labeled NB500-178AF647
Alexa Fluor (R) 700 Labeled NB500-178AF700
Biotin Labeled NB500-178B
DyLight 405 Labeled NB500-178V
DyLight 488 Labeled NB500-178G
DyLight 550 Labeled NB500-178R
DyLight 650 Labeled NB500-178C
FITC Labeled NB500-178F
HRP Labeled NB500-178H

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Contact Information

Product PDFs


Gene Symbol NUMB

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