Numb Antibody

Images

 
Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.
Immunocytochemistry/ Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Ch, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
3.5 mg/ml

Numb Antibody Summary

Background
Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggest that mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different pro...cesses. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.
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Immunogen
A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
Localization
Membrane-associated
Specificity
This is specific for all four isoforms of the NUMB protein.
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-0.5 ug/ml
  • Simple Western 1:200
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This NUMB antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
A-431 Whole Cell Lysate
HeLa Whole Cell Lysate
Control Peptide
Numb Peptide (NB500-178PEP)
Publications
Read Publications using
NB500-178 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, mouse and chicken. Immunogen sequence has 90% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
3.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Numb Antibody

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-178
Species: Hu, Mu, Ch, Rt
Applications: WB, Simple Western, ICC/IF

Publications for Numb Antibody (NB500-178)(3)

Reviews for Numb Antibody (NB500-178) (0)

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Product General Protocols

View specific protocols for Numb Antibody (NB500-178): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Numb Antibody (NB500-178). (Showing 1 - 2 of 2 FAQs).

  1. I have looked at the data sheet for NB500-178 and do not see any reference to IP, but for some reason it was listed on our data sheet. Do you by chance have any reports of attempted use of the antibody in IP or any historical information regarding a listing for IP that might be helpful?
    • I pulled data on this target and I am not showing any notes of it being removed or ever being tested in that application. So unfortunately I have nothing to provide.
  2. I'm using your NUMB Antibody, cat# NB500-178. Does it pick up Numb Like protein also?
    • We performed an alignment of the immunogen sequence against the numb like protein for mouse based on UniProt. We show an approximately 50% similarity between the two sequences. As a result, we do not believe this antibody will recognize Numb-like protein. However, we have to stipulate that the lab has not tested the binding between the numb like protein and this antibody directly. Therefore, we cannot say with certainty whether the numb like protein will also be detected. Especially due to the fact that numb like protein has a similar molecular weight to one of the isoforms of the numb protein this antibody targets making it more difficult to analyze. Based on the sequence analysis information, it appears unlikely that the antibody detects numb like protein.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-178AF350
Alexa Fluor 405 NB500-178AF405
Alexa Fluor 488 NB500-178AF488
Alexa Fluor 532 NB500-178AF532
Alexa Fluor 594 NB500-178AF594
Alexa Fluor 647 NB500-178AF647
Alexa Fluor 700 NB500-178AF700
Alexa Fluor 750 NB500-178AF750
Biotin NB500-178B
DyLight 350 NB500-178UV
DyLight 405 NB500-178V
DyLight 488 NB500-178G
DyLight 550 NB500-178R
DyLight 594 NB500-178DL594
DyLight 650 NB500-178C
DyLight 680 NB500-178FR
DyLight 755 NB500-178IR
FITC NB500-178F
HRP NB500-178H
Janelia Fluor 549 NB500-178JF549
Janelia Fluor 646 NB500-178JF646

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Bioinformatics

Gene Symbol NUMB