Numb Antibody

Images

 
Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.
Immunocytochemistry/ Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunocytochemistry/ Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Immunocytochemistry/ Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Ch, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.0 mg/ml

Order Details

Numb Antibody Summary

Background
Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggest that mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different pro...cesses. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.
Show More
Immunogen
A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
Localization
Membrane-associated
Specificity
This is specific for all four isoforms of the NUMB protein.
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Simple Western 1:200
  • Western Blot 0.2-0.5 ug/ml
Application Notes
In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
A-431 Whole Cell Lysate
Control Peptide
Numb Peptide (NB500-178PEP)
Publications
Read Publications using
NB500-178 in the following applications:

Reactivity Notes

Immunogen sequence has 90% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Numb Antibody

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Numb Antibody (NB500-178)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB500-178 Applications Species
Das A, Adhikary S, Chowdhury AR, Barui A Chirality-induced Lineage Enforcement of Mechanosensitive Mesenchymal Stem Cells Across Germ Layer Boundaries Stem cell reviews and reports 2023-11-16 [PMID: 37971671]
Das A, Adhikary S, Chowdhury AR Et al. Leveraging Substrate Stiffness to Promote Stem Cell Asymmetric Division via Mechanotransduction-Polarity Protein Axis and Its Bayesian Regression Analysis Rejuvenation Res 2022-03-22 [PMID: 35316074] (ICC/IF)

Details:
Citation using the Texas Red version of this antibody.
ICC/IF
Huang SC, Vu LV, Yu FH Et al. Multifunctional protein 4.1R regulates the asymmetric segregation of Numb during terminal erythroid maturation The Journal of biological chemistry 2021-08-06 [PMID: 34364872]
Wang H, Xiang D, Liu B et al. Inadequate DNA Damage Repair Promotes Mammary Transdifferentiation, Leading to BRCA1 Breast Cancer Cell 2019-06-27 [PMID: 31251913] (Mouse) Mouse
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)

Details:
Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
Human
Lenihan JA, Saha O, Young PW et al. Proteomic analysis reveals novel ligands and substrates for LNX1 E3 ubiquitin ligase PLoS One. 2017-11-08 [PMID: 29121065] (WB, Human) WB Human
Lenihan JA, Saha O, Heimer-McGinn V et al. Decreased Anxiety-Related Behaviour but Apparently Unperturbed NUMB Function in Ligand of NUMB Protein-X (LNX) 1/2 Double Knockout Mice. Mol. Neurobiol. 2016-11-26 [PMID: 27889896] (WB, Mouse) WB Mouse

Reviews for Numb Antibody (NB500-178) (0)

There are no reviews for Numb Antibody (NB500-178). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Numb Antibody (NB500-178): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Numb Antibody (NB500-178). (Showing 1 - 2 of 2 FAQs).

  1. I have looked at the data sheet for NB500-178 and do not see any reference to IP, but for some reason it was listed on our data sheet. Do you by chance have any reports of attempted use of the antibody in IP or any historical information regarding a listing for IP that might be helpful?
    • I pulled data on this target and I am not showing any notes of it being removed or ever being tested in that application. So unfortunately I have nothing to provide.
  2. I'm using your NUMB Antibody, cat# NB500-178. Does it pick up Numb Like protein also?
    • We performed an alignment of the immunogen sequence against the numb like protein for mouse based on UniProt. We show an approximately 50% similarity between the two sequences. As a result, we do not believe this antibody will recognize Numb-like protein. However, we have to stipulate that the lab has not tested the binding between the numb like protein and this antibody directly. Therefore, we cannot say with certainty whether the numb like protein will also be detected. Especially due to the fact that numb like protein has a similar molecular weight to one of the isoforms of the numb protein this antibody targets making it more difficult to analyze. Based on the sequence analysis information, it appears unlikely that the antibody detects numb like protein.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Numb Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NUMB