Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.
Immunocytochemistry/ Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggest that mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different pro...cesses. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.
A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
This is specific for all four isoforms of the NUMB protein.
Rat (90%). Backed by our 100% Guarantee.
Immunogen affinity purified
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This NUMB antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
FAQs for Numb Antibody (NB500-178). (Showing 1 - 2 of 2 FAQs).
I have looked at the data sheet for NB500-178 and do not see any reference to IP, but for some reason it was listed on our data sheet. Do you by chance have any reports of attempted use of the antibody in IP or any historical information regarding a listing for IP that might be helpful?
I pulled data on this target and I am not showing any notes of it being removed or ever being tested in that application. So unfortunately I have nothing to provide.
I'm using your NUMB Antibody, cat# NB500-178. Does it pick up Numb Like protein also?
We performed an alignment of the immunogen sequence against the numb like protein for mouse based on UniProt. We show an approximately 50% similarity between the two sequences. As a result, we do not believe this antibody will recognize Numb-like protein. However, we have to stipulate that the lab has not tested the binding between the numb like protein and this antibody directly. Therefore, we cannot say with certainty whether the numb like protein will also be detected. Especially due to the fact that numb like protein has a similar molecular weight to one of the isoforms of the numb protein this antibody targets making it more difficult to analyze. Based on the sequence analysis information, it appears unlikely that the antibody detects numb like protein.
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