Numb Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Ch, RtSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Numb Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
Localization
Membrane-associated
Specificity
This is specific for all four isoforms of the NUMB protein.
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
Application Notes
This NUMB antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.

Reactivity Notes

Human, mouse and chicken. Immunogen sequence has 90% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Numb Antibody [FITC]

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

Numb is an evolutionary conserved protein which plays a crucial role in cell fate determination, an important event in embryonic development as well as in certain pathologies including cancer. Numb was initially identified in Drosophila (dNumb) as a membrane-associated protein which asymmetrically segregates in dividing cells and define distinct cell fates by interacting with/inhibiting Notch. dNumb's mammalian homologs (encoded by two genes mNumb and mNumb-like) displays a higher degree of complexity with alternative spliced isoforms, and display multitude of functions such as control of asymmetric cell division and cell fate choice, endocytosis, cell adhesion, cell migration, ubiquitination of specific substrates and regulation of Notch, Hedgehog, p53 etc. The multiple functions of Numb highlight its critical role not only in the control of physiological processes, but also in diseases, when deregulated. The ability of Numb to control oncogenic (Notch and Hedgehog) and tumor suppressor (p53) pathways provides a strong link between deregulated Numb function and cancer. Indeed, hyperactive Notch and Hedgehog functions have been described to be responsible of several tumors including breast cancer and medulloblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NUMB
Entrez