NT-4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

NT-4 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-4
  Gly81-Ala210
  Accession # P34130
• Specificity: Detects human NT-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) CNTF, rhNeurturin, rhNT-3, rhBDNF, rhGDNF, and rh beta -NGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NTF4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human NT-4 Antibody (Catalog # MAB268)
  Standard: Recombinant Human NT-4 (Catalog # 268-N4)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NT-4 Antibody [Biotin]

• GLC10
• GLC1O
• neurotrophic factor 4
• neurotrophic factor 5
• neurotrophin 4
• neurotrophin 5 (neurotrophin 4/5)
• neurotrophin-4
• neurotrophin-5
• Neutrophic factor 4
• NT4
• NT-4
• NT-4/5
• NT-5
• NTF4
• NTF5
• NTF5NT5

Background

Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1‑3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48‑52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8‑10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8‑10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14‑16).

1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NT-4 Antibody (BAF268)(1)

Publication using BAF268

• Nelson PG, Kuddo T, Song EY Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome. Int. J. Dev. Neurosci., 2005-11-14;24(1):73-80. 2005-11-14 [PMID: 16289943] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: NTF4
• Uniprot:
  • P34130()