CNTFR alpha Antibody

Images

 
Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recom­binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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CNTFR alpha Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
Gln23-Pro346 (predicted)
Accession # P26992
Specificity
Detects human CNTF R alpha  in direct ELISAs and Western blots. In Western blots, greater than 50% cross-reactivity with recombinant rat CNTF sR alpha is observed and less than 5% cross-reactivity with recombinant human (rh) IL-2 sR gamma and rhIL-6 sR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CNTFR
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 10-20 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF-303-NA in the following applications:

Publications
Read Publications using
AF-303-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTFR alpha Antibody

  • CNTF R alpha
  • CNTFR
  • CNTFRa

Background

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CNTFR alpha Antibody (AF-303-NA)(4)

We have publications tested in 2 confirmed species: Human, Feline.

We have publications tested in 3 applications: IHC Frozen, Immunoprecipitation, Western Blot.


Filter By Application
IHC Frozen
(1)
Immunoprecipitation
(1)
Western Blot
(2)
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Human
(3)
Feline
(1)
All Species

Review for CNTFR alpha Antibody (AF-303-NA) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF-303-NA:
Filter by Applications
IF
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 12/19/2014
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Summary

ApplicationImmunofluorescence
Sample TestedMouse neurons DIV12
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1 ug/ml

Details

Detection Notesn/a
Fixation DetailsPFA-fixed

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/

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FAQs for CNTFR alpha Antibody (AF-303-NA) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
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4
1
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1
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Anonymous
12/19/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CNTFR
Uniprot