CNTFR alpha Antibody [Unconjugated]

Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recom­binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom­binant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CNTFR alpha Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
  Gln23-Pro346 (predicted)
  Accession # P26992
• Specificity: Detects human CNTF R alpha in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNTFR
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 10-20 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTFR alpha Antibody [Unconjugated]

• CNTF R alpha
• CNTFR alpha
• CNTFR
• CNTFRa

Background

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNTFR alpha Antibody (AF-303-NA)(4)

Publications using AF-303-NA

• Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer. Mol. Cell. Biol., 2010;17(0):4175. 2010 [PMID: 20584990] (Western Blot, Human)
• Yata T, Nakamura M, Sagawa H, Tokita Y, Terasaki H, Watanabe M Survival and axonal regeneration of off-center retinal ganglion cells of adult cats are promoted with an anti-glaucoma drug, nipradilol, but not BDNF and CNTF. Neuroscience, 2007;148(1):53-64. 2007 [PMID: 17629411] (IHC-Fr, Feline)
• He W, Gong K, Smith DK, Ip NY The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling. FEBS Lett., 2005;579(20):4317-23. 2005 [PMID: 16051226] (Western Blot, Human)
• Koh SW Ciliary neurotrophic factor released by corneal endothelium surviving oxidative stress ex vivo. Invest. Ophthalmol. Vis. Sci., 2002;43(9):2887-96. 2002 [PMID: 12202507] (Immunoprecipitation, Human)

Review for CNTFR alpha Antibody (AF-303-NA) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: cynthia morimoto IF Mouse 12/19/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse neurons DIV12
• Species: Mouse

Bioinformatics

• Gene Symbol: CNTFR
• Uniprot:
  • P26992()