Sortilin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sortilin Antigen ...read more
Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 122 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ...read more
Sortilin was detected in perfusion fixed frozen sections of mouse brain (rostral ventral medulla) using Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) at 1.7 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC, Block
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Sortilin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sortilin
Gly76-Asn753
Accession # Q6PHU5
Specificity
Detects mouse Sortilin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Sortilin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SORT1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Blockade of Receptor-ligand Interaction
Reviewed Applications
Read 6 Reviews rated 5
using
AF2934 in the following applications:

Publications
Read Publications using
AF2934 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sortilin Antibody [Unconjugated]

  • 100 kDa NT receptor
  • Glycoprotein 95
  • Gp95
  • Gp95LDLCQ6
  • Neurotensin receptor 3
  • NT3NTR3
  • Ntr3
  • SORT1
  • sortilin 1
  • Sortilin

Background

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Mouse preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre‑ and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature mouse Sortilin shares 98% amino acid (aa) identity with rat, and 91% aa identity with human and canine sortilin. During murine development, sortilin is mainly expressed in the nervous system (5), where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6‑9). ProNGF (or the NGF propeptide alone) binds sortilin with a much higher affinity (KD ~5-8 nM) than does mature NGF (KD ~90 nM). The complex of sortilin, pro‑NGF and the receptor p75ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with proBDNF and BDNF (8‑9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sortilin Antibody (AF2934)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, ICC Fresh, WB.


Filter By Application
ICC
(2)
ICC Fresh
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species

Reviews for Sortilin Antibody (AF2934) (6) 56

Average Rating: 5
(Based on 6 reviews)
We have 6 reviews tested in 1 species: Mouse.

Reviews using AF2934:
Filter by Applications
IHC-Fr
(3)
IF
(1)
IP
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(6)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 12/19/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedDorsal root ganglia
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 12/19/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary dorsal root ganglia neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IHC-Fr Mouse 12/19/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSpinal cord cryosections and dorsal root ganglia
SpeciesMouse
 
reviewed by:
Anonymous
IP Mouse 12/19/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedEndogenous mouse sortilin
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
Immunocytochemistry Sortilin AF2934
Enlarge
reviewed by:
Anonymous
ICC Mouse 12/19/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary neurons
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IHC-Fr Mouse 12/19/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedCryosections
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Sortilin Antibody (AF2934) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Sortilin Antibody (AF2934)

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Diseases for Sortilin Antibody (AF2934)

Discover more about diseases related to Sortilin Antibody (AF2934).
 

Pathways for Sortilin Antibody (AF2934)

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Recent Reviews

5
5
6
4
0
3
0
2
0
1
0

 
Anonymous
12/19/2014
Application: IHC-Fr
Species: Mouse

 
Anonymous
12/19/2014
Application: IF
Species: Mouse

 
Anonymous
12/19/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol SORT1
Entrez
Uniprot