NRIP Antibody - BSA Free

Immunoprecipitation: NRIP Antibody [NBP1-30075] - Whole cell lysate from HeLa cells. NBP1-30075 used for IP at 10 mcg/mg lysate. NRIP was also immunoprecipitated by NBP1-30076. For blotting immunoprecipitated NRIP, NBP1-30076 was used at 1 mcg/ml.

Immunohistochemistry-Paraffin: NRIP Antibody [NBP1-30075] - FFPE section of human breast carcinoma.Antibody: Affinity purified rabbit anti- NRIP used at a dilution of 1:1,000 (1ug/ml). Detection:DAB

• Reactivity: Hu, Mu, Pm-Or
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NRIP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NRIP Antibody - BSA Free (NBP1-30075) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Maps to a region between residue 300 and 350 of human nuclear receptor interaction protein (IQ motif and WD repeats 1) using the numbering given in entry NP_001017977.1
• Predicted Species: Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCAF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 10-15 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended, use rabbit anti-NRIP antibody NBP1-30076.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NRIP Antibody - BSA Free

• 1200006M05Rik
• Androgen receptor complex-associated protein
• ARCAP
• DDB1 and CUL4 associated factor 6
• DDB1- and CUL4-associated factor 6
• FLJ23798
• IQ motif and WD repeat-containing protein 1
• IQ motif and WD repeats 1
• IQWD1
• MSTP055
• NRIP
• Nuclear receptor interaction protein
• PC326

Background

WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies. Their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860a.a nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up-regulates their transcriptional activity, thereby functioning as a nuclear receptor co-activator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer, thus indicating a potential therapeutic intervention. Northern Blot analysis detects a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCAF6
• Entrez:
  • 55827(Human)
• Uniprot:
  • Q58WW2(Human)