| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58849SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58851SS | |||
HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Nox4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8158) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nox4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8158-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41293-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99109-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99108-50ul | |||
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Western-Blot-NB110-58849-img0013.jpg "Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.")
![Immunocytochemistry/Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58849] - Porcine aortic endothelial cells stained with NB110-58849 (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58849-img0019.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in HUVEC whole cell lysate. Lanes 1 and 2: serum-starved HUVEC lysate denatured at 95C for 5 minutes. Lanes 3: serum-starved HUVEC lysate denatured at room temperature for 10 minutes. Image from verified customer review.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Western-Blot-NB110-58849-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in mouse brain showing positive staining in neurons in the cortex. Image provided by Rachel Reith.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58849-img0011.jpg)
![Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58849] - Detection of NOX4 in proximal convoluted tubules of the kidney using NB110-58849 at 5 ug/mL.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Immunohistochemistry-NB110-58849-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Nox4 Antibody [NB110-58849] - Silencing of NOX4 in H4-II-C3 rat hepatoma cell line. Image from verified customer review.](http://images.novusbio.com/fullsize/Nox4-Antibody---BSA-Free-Knockdown-Validated-NB110-58849-img0020.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Effect of metformin on bleomycin-induced lung fibrosis development in mice. f WB using anti-NOX4, & anti-beta -actin of cell lysates from normal LF (lane 1, 2, 3) & IPF LF (lane 4, 5, 6). Lower panel is average (±SEM) taken from 3patients shown as relative expression. Open bar is normal LF & filled bar is IPF LF. *p < 0.05 Image collected & cropped by CiteAb from following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-016-0420-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415371992.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Fasting protects against oxidative stress resulting after two weeks of unilateral ischemia-reperfusion (IR) injury. (A) Representative kidney sections (10×) immunostained for 8-hydroxy-2′-deoxyguanosine (8-OHdG) in sham, IR, & IR + Fasting experimental groups at day 14 post-IR or sham surgery. (B) Immunoblots (left) & quantification (right) of catalase (CAT), glutamate-cysteine ligase modifier subunit (GCLM), & nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) in the kidney cortex of rats from sham, IR, & IR + Fasting experimental groups. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Data are expressed as mean ± SEM. n = 3 animals per group. * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31443530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415384183.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - SPA0355 decreased renal oxidative stress & regulated levels of pro-oxidant & antioxidant enzymes in LPS-treated mice. (A) Representative images of IHC of 4-hydroxynonenal (4-HNE) in kidneys. Bar = 20 μm. (B) Percentage of 4-HNE-positive area per field. (C) Renal levels of malondialdehyde (MDA). (D) Representative images of Western blotting of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) & GAPDH. (E) The mRNA levels of manganese superoxide dismutase (MnSOD) & catalase. Results are from 8 mice per group (biological replicates) & 2 or 3 technical replicates per mouse. *** p < 0.001 vs. vehicle-treated mice (Veh). ###p <0.001 vs. LPS-injected mice (LPS). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32635491), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415395925.jpg)
![Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58849] - Presence of NOX4 in human ovarian tissue. Immunohistochemistry using human ovarian sections & an anti-NOX4 antibody from ProSci showed positive staining for NOX4 in granulosa (GC) & theca cells (TC) of a secondary follicle (A), of a small antral follicle (B), of a large antral follicle (C) as well as in luteinized GCs (LGC) & luteinized TCs (LTC) of the corpus luteum (E). Serum controls lacked first antibody (D & F). Scale bars: A–E = 30 µm, F = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30837663), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415371990.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Role of NADPH oxidases in IH-induced HIF-2 alpha degradation. A. Effect of NADPH oxidase (Nox) inhibitors Apocynin (Apo, 1 mM) & AEBSF (15 µM) on HIF-2 alpha protein following exposure to IH. B. HIF-2 alpha expression in PC12 cells transfected with Nox2 & Nox4 siRNA & exposed to normoxia (N) or IH. Tubulin expression was monitored as control for protein loading. Bottom panels of A & B represent average data of densitometric analysis of the immunoblots presented as mean ± S.E.M from three independent experiments. *p<0.05; n.s. not significant, p>0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24124516), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415384124.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - The representative images of immunohistochemical staining (A–D; sections were counterstained with hematoxylin; original magnification, × 20), & western blot assays (E,F) showed that FLL treatment decreased Nox4 expression in tibias & uteri of OVX rats (n = 9). In addition, FLL treatment also decreased cytochrome C (Cyto-C; G) & increased Bcl-2 expression (H) in the tibias of OVX rats (n = 9). Data are presented as mean ± SD. IOD denotes integrated optical density of interested areas. #p < 0.05 with Sham group, *p < 0.05 compared with OVX group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28588482), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-310202415363718.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Blockade of LTCC suppressed CaMKII-NF-kB pathway in DOX-treated hearts. (a) Representative immunoblots & quantitative analysis of CaMKII, phosphorylated CaMKII, & GAPDH in DOX (3 doses of DOX at 6 mg/kg body weight every third day for 1 week) or control vehicle (phosphate-buffered saline: PBS) treated-C57B/6 J mouse hearts subjected to either nifedipine (Nif, 10 mg/day/day) or saline for 9 days (n = 5). (b) Representative immunoblots & quantitative analysis of NF-kappa B, phosphorylated NF-kappa B, cleaved caspase 3, & GAPDH in each group (n = 5). The experiment was conducted 3 times. (c–g) Representative immunoblotsa & quantitative analysis of ERK, phosphorylated ERK, JNK, phosphorylated JNK, Nox4, p53, & GAPDH in each group (n = 5). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31285514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-31020241535677.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Diabetic atrophied muscles exhibited a state of heightened oxidative stress (HSOS). (a & b) Superoxide generation was measured in frozen muscle sections of control & diabetic using dihydroethidium-based confocal microscopic staining technique. (c & d) NADPH oxidase in a membrane fraction was assessed according to procedure involving the substrate NADPH & lucigenin chemiluminescence or the Amplex Red/horseradish peroxidase fluorescence-based assays. (e & f) Muscle NADPH oxidase-related isoforms including NOX2 & NOX4 were determined at the mRNA (e) & protein levels (f) using RT-PCR & Western blotting-based techniques. (g) Mitochondrial H2O2 generation at the steady state level & in the presence of added glutamate/malate substrates was measured using the Amplex Red/horseradish peroxidase fluorescence-based assay (g). Activities of complexes I (h) & III (i) of the electron transport chain were measured using spectrophotometric-based assay. Abbreviation: C: control; D: diabetic. Values are means ± SEM for at least 6 animals/group. ∗Significantly different from corresponding control values at P ≤ 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510624), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-31020241624922.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Characterization of NOX4 & PKM2 in human RCC tumors & adjacent tissue. a Mitochondrial fractions were prepared from human tumors (T) or uninvolved adjacent tissue (N). NOX4 expression was examined by western blot analysis. Prohibitin was probed as a mitochondrial marker & loading control. b Quantitation of NOX4 distribution in the mitochondrial fraction from a. The results are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ± S.E.M. *p < 0.05 compared to normal (N). c Mitochondria fractions were prepared from RCC tumors & NADPH-dependent superoxide generation was examined in the presence (+) or absence (−) of ATP. The results are from eight tumors & are expressed as the means using one-way ANOVA with Tukey’s post hoc test where ±S.E.M. **p < 0.01 is compared to without (−) ATP. d PKM2 & PKM1 expression was examined by western blot analysis in lysates prepared from human tumors (T) or uninvolved adjacent tissue (N) from the same patient. Actin as loading control Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29051480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-31020241624925.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - Fbs stimulated by BMM differentiate into myofibroblasts via the Cyr61/Nox4 pathway. (A) Western blotting for Fb differentiation, involving analysis of specific factors such as FSP-1, NOX2, NOX4, collagen-1, & Cyr61. The expression of these factors was normalized to that of GAPDH; (B) Cells were treated with BMM for 3 or 6 h or DPI (5 μM; NOX inhibitor) for 1 h or were co-treated with DPI & BMM. ROS production was measured using the DCF-DA assay & calculated as a percentage of the mean fluorescence intensity compared with that of the control. * p < 0.05 as compared to the control; # p < 0.05 as compared to the BMM (6 h)-treated group; (C) Cells were treated with BMM for 3 h or DPI (5 μM, NOX inhibitor) for 1 h or were co-treated with DPI & BMM. Cyr61 expression was normalized to that of GAPDH after western blotting. The values indicate intensities of protein expression with respect to that of the loading control; (D) Secretion of MMPs from BMM-treated-Fbs by using conditioned medium, followed by western blotting analysis. The expression levels were normalized to PonceauS, used as a loading control. *** p < 0.001 as compared to the control. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/4/1164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-31020241624917.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - DSC restores redox balance in colonic tissues. A, Colitis was induced as described in Materials & Methods & treated with or without DSC (50 mg/kg). Representative bands & densitometry analysis of Nrf2 nuclear translocation & HO‐1 expression in colonic tissues. Histone H3 & beta ‐actin were used as loading control, respectively. B, Colitis was induced as described in Materials & Methods & treated with lentiviral Nox4 shRNA or lentiviral scrambled shRNA. Representative bands & densitometry analysis of Nrf2 nuclear translocation & HO‐1 expression in colonic tissues. Histone H3 & beta ‐actin were used as loading control, respectively. BMDM was stimulated with or without DSC (50 μmol/L) as described in Materials & Methods. C, Representative bands & densitometry analysis of Nox4. beta ‐actin was used as loading control. D, Representative bands & densitometry analysis of Nrf2 nuclear translocation & cytoplasmic HO‐1 expression in BMDM. beta ‐actin or histone H3 was used as loading control. Data shown are means ± SEM of n = 8 in each group. *P < 0.05 vs Control cell or mice (CTL), #P < 0.05 vs DSS‐treated mice or LPS‐stimulated cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32945118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-3102024162493.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58849] - DSC ameliorates Nox4 expression & ROS production. A, Colitis was induced as described in Materials & Methods, & the colonic tissues were collected as indicated periods. Representative bands & quantitative analysis of Nox4 in colonic tissues were shown. B‐F, Colitis was induced as described in Materials & Methods & treated with or without DSC (50 mg/kg). B, Representative bands & quantitative analysis of Nox4 in colonic tissues. C, Representative images & quantitative analysis of ROS production by DHE staining in colonic tissues, scale bar = 100 μm. D, Quantitative analysis of GSH/GSSG ratio. E, Quantitative analysis of tissue H2O2 production. Colitis was induced as described in Materials & Methods & treated with or lentiviral Nox4 shRNA. F, Representative bands & quantitative analysis of Nox4 in colonic tissues. G, Representative images & quantitative analysis of colon length. H, Representative images of H&E staining & histological score. I, Representative bands & quantitative analysis of ZO‐2 & claudin‐1 expression in colonic tissues. beta ‐actin was used as loading control. Data shown are means ± SEM of n = 8 in each group. *P < 0.05 vs Control (CTL), #P < 0.05 vs DSS‐treated mice Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32945118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58849_rabbit-polyclonal-nox4-antibody-31020241624928.jpg)
![Western Blot: Nox4 Antibody [NB110-58851] - Whole cell protein from human HeLa, Hek293, HepG2, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml anti-Nox4 in 1% BSA and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Nox4 is detected at approx. 70 kDa (arrowhead)](http://images.novusbio.com/fullsize/Nox4-Antibody-Western-Blot-NB110-58851-img0015.jpg "Western Blot: Nox4 Antibody [NB110-58851] - Whole cell protein from human HeLa, Hek293, HepG2, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml anti-Nox4 in 1% BSA and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Nox4 is detected at approx. 70 kDa (arrowhead)")
![Immunocytochemistry/Immunofluorescence: Nox4 Antibody [NB110-58851] - HeLa cells were fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with antibodies to Nox4 (NB110-58851) and the ER marker Calreticulin (NBP1-47518), each at 20 ug/ml for 1 hour at room temperature. The coverslips were washed 3x in PBS and incubated with Alexa-Fluor 488 anti-rabbit secondary antibody. (green) and DyLight 550 anti-mouse secondary antibody. The merged image shows the co-localization of Nox4 and Calreticulin in the ER.](http://images.novusbio.com/fullsize/Nox4-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58851-img0012.jpg)
![Immunohistochemistry: Nox4 Antibody [NB110-58851] - Analysis using the Biotin conjugate of NB110-58851. Staining of NOX4 in proximal convoluted tubules of human kidney.](http://images.novusbio.com/fullsize/Nox4-Antibody-Immunohistochemistry-NB110-58851-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nox4 Antibody [NB110-58851] - HeLa cells were fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with NB110-58851 at 20 ug/ml for 1 hour at room temperature, washed 3x in PBS and incubated with Alexa-Fluor 488 anti-rabbit secondary antibody. Nox4 (Green) was detected in the ER.](http://images.novusbio.com/fullsize/Nox4-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58851-img0013.jpg)
![Western Blot: Nox4 Antibody [NB110-58851] - Analysis using the Biotin conjugate of NB110-58851. Detection of NOX4 in human kidney lysates uisng NB110-58851 at 0.5 ug/ml. Band observed at 31 kDa may represent reported splice isoform.](http://images.novusbio.com/fullsize/Nox4-Antibody-Western-Blot-NB110-58851-img0016.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58851] - Small interfering RNA against Nox4 (siNox4) alleviated oxidative stress & DNA damage induced by high oxygen tension in NP cells. (a, b) RT-qPCR analysis (N = 3) & representative immunoblot analysis of Nox4 in NP cells. The knockdown of Nox4 in NP cells was confirmed. (c) ROS production in NP cells (N = 3). (d) RT-qPCR analysis of MsrbA, MsrB1, & MsrB2 in NP cells (N = 3). (e, f) Immunofluorescence staining of gamma -H2A.X & percentage of gamma -H2A.X-positive cells in NP cells (N = 6). NP cells were transfected with siNox4 or scrambled siRNA control (siCtrl) before high oxygen tension treatment. ∗, P value < 0.05, error bars represent standard error. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29147462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58851_rabbit-polyclonal-nox4-antibody-310202415395922.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58851] - Nox4 overexpression boosted ROS production & induced DNA damage in NP cells. (a) RT-qPCR analysis (N = 4) of Nox4, MsrB1, & MsrB2 in NP cells overexpressing Nox4. (b) Representative immunoblot analysis of Nox4 in NP cells overexpressing Nox4. (c) ROS production in NP cells overexpressing Nox4 (N = 3). (d, e) Immunofluorescence staining of gamma -H2A.X & percentage of gamma -H2A.X-positive cells in NP cells overexpressing Nox4 (N = 8). NP cells were transfected with Nox4 vectors for Nox4 overexpression. ∗, P value < 0.05, error bars represent standard error. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29147462), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58851_rabbit-polyclonal-nox4-antibody-310202415171335.jpg)
![Western Blot: Nox4 Antibody - BSA Free [NB110-58851] - Alternative splicing of NOX4 in rat kidney & heart (A). Alternative splicing of NOX4 in human hearts (B). Quantitative evaluation of spliced NOX4 isoforms in ICM samples (C). Quantitative evaluation of spliced NOX4 isoforms in DCM samples (D). Data are mean ± S.E.M. n = 5/group. *p < 0.05. LV, left ventricle; IVS, interventricular septum; RV, right ventricle; ICM, ischemic cardiomyopathy; DCM, dilated cardiomyopathy. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29204124), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-58851_rabbit-polyclonal-nox4-antibody-310202415371935.jpg)
![Western Blot: Nox4 Antibody [NBP2-41293] - Analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Nox4-Antibody-Western-Blot-NBP2-41293-img0001.jpg "Western Blot: Nox4 Antibody [NBP2-41293] - Analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: Nox4 Antibody - BSA Free [NBP2-41293] - Immunohistochemistry of Nox4 in human spleen tissue with Nox4 antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41293_rabbit-polyclonal-nox4-antibody-271120241937785.jpg)
![Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NBP2-41293] - Immunofluorescence of Nox4 in human spleen tissue with Nox4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41293_rabbit-polyclonal-nox4-antibody-271120242013248.jpg)
![ELISA: Human Nox4 ELISA Kit (Colorimetric) [NBP2-76790] - Samples were spiked with high concentrations of Human Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76790_human-nox4-elisa-kit-colorimetric-132202416254027.jpg "ELISA: Human Nox4 ELISA Kit (Colorimetric) [NBP2-76790] - Samples were spiked with high concentrations of Human Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Nox4 ELISA Kit (Colorimetric) [NBP2-76790] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76790_human-nox4-elisa-kit-colorimetric-4320248582582.jpg)
![ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Samples were spiked with high concentrations of Rat Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76792_rat-nox4-elisa-kit-colorimetric-13220241620486.jpg "ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Samples were spiked with high concentrations of Rat Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Nox4 ELISA Kit (Colorimetric) [NBP2-76792] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76792_rat-nox4-elisa-kit-colorimetric-4320249192833.jpg)
![ELISA: Mouse Nox4 ELISA Kit (Colorimetric) [NBP2-76791] - Samples were spiked with high concentrations of Mouse Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76791_mouse-nox4-elisa-kit-colorimetric-13220241681473.jpg "ELISA: Mouse Nox4 ELISA Kit (Colorimetric) [NBP2-76791] - Samples were spiked with high concentrations of Mouse Nox4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Nox4 ELISA Kit (Colorimetric) [NBP2-76791] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76791_mouse-nox4-elisa-kit-colorimetric-432024973680.jpg)
![Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOX4.](http://images.novusbio.com/fullsize/NOX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04362-img0002.jpg "Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOX4.")
![Western Blot: NOX4 Overexpression Lysate (Adult Normal) [NBP2-04362] - Left - Empty vector transfection control lysate (HEKP293 cell lysate); Right - NOX4 Over-expression Lysate,](http://images.novusbio.com/fullsize/NOX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04362-img0001.jpg)
![Western Blot: Nox4 Antibody [NBP2-99109] - Anti-Nox4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa](http://images.novusbio.com/fullsize/Nox4-Antibody-Western-Blot-NBP2-99109-img0001.jpg "Western Blot: Nox4 Antibody [NBP2-99109] - Anti-Nox4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa")
![Immunoprecipitation: Nox4 Antibody [NBP2-99108] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-NOX4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NOX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 64 kDaObserved band size: 71 kDa](http://images.novusbio.com/fullsize/Nox4-Antibody-Immunoprecipitation-NBP2-99108-img0001.jpg "Immunoprecipitation: Nox4 Antibody [NBP2-99108] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-NOX4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NOX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 64 kDaObserved band size: 71 kDa")
