DCTN3 Antibody

Images

 
Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.03ug/ml) of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Western Blot: DCTN3 Antibody [NBP1-52105] - Staining (0.2ug/ml) of Mouse (A) and Rat (B) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DCTN3 Antibody [NBP1-52105] - Staining (8ug/ml) of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DCTN3 Antibody Summary

Immunogen
Peptide with sequence C-QVALGNISSKRE corresponding to internal region according to NP_009165.1; NP_077324.1.
Epitope
C-QVALGNISSKRE
Specificity
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Predicted Species
Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCTN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6 - 8 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx 20 kDa band observed in Human Liver lysates, and approx. 18 kDa in Rat Kidney lysates and in preliminary testing of Mouse Kidney lysate (calculated MW of 19. 5kDa according to NP_077324.1 and 21.1 kDa according to NP_001102129.1.
Publications
Read Publication using NBP1-52105.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DCTN3 Antibody

  • DCTN22DCTN-22
  • dynactin 3 (p22)
  • dynactin 3
  • Dynactin complex subunit 22 kDa subunit
  • dynactin light chain
  • dynactin subunit 3
  • MGC111190
  • p22

Background

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5758
Species: Hu
Applications: ICC, IHC, WB
NBP1-40974
Species: Hu
Applications: ELISA, Flow, IHC, IHC-Fr, IP, WB
NBP1-89163
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-1343
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP2-13677
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB1904
Species: Hu
Applications: ICC, IHC, WB
NBP1-41012
Species: Hu
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
1137-SL
Species: Hu
Applications: BA
NBP1-82454
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-80467
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00006812-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
AF2377
Species: Mu
Applications: IHC, Simple Western, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-790
Species: Ba, Hu, Mu, Po
Applications: Flow, IF, IHC, IHC-P, IP, PEP-ELISA, WB
NB110-58849
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt, Sh
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
NBP3-13279
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-46085
Species: Hu
Applications: IHC, WB
NBP1-52105
Species: Hu, Rt, Mu, Bv, Ca
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for DCTN3 Antibody (NBP1-52105)(1)

Reviews for DCTN3 Antibody (NBP1-52105) (0)

There are no reviews for DCTN3 Antibody (NBP1-52105). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DCTN3 Antibody (NBP1-52105) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DCTN3 Products

Bioinformatics Tool for DCTN3 Antibody (NBP1-52105)

Discover related pathways, diseases and genes to DCTN3 Antibody (NBP1-52105). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DCTN3 Antibody (NBP1-52105)

Discover more about diseases related to DCTN3 Antibody (NBP1-52105).

Research Areas for DCTN3 Antibody (NBP1-52105)

Find related products by research area.

Blogs on DCTN3

There are no specific blogs for DCTN3, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DCTN3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol DCTN3
Entrez
Uniprot