DCTN3 Antibody

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Western Blot: DCTN3 Antibody [NBP1-52105] - Analysis of DCTN3 in Human Kidney lysate (35ug protein in RIPA buffer) using NBP1-52105 at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DCTN3 Antibody Summary

Immunogen
Peptide with sequence C-QVALGNISSKRE corresponding to internal region according to NP_077324.1.
Epitope
C-QVALGNISSKRE
Specificity
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DCTN3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 20 kDa band observed in human kidney, skeletal muscle and pancreas lysates (calculated MW of 19.5 kDa band according to NP_077324.1). In addition, a 25 kDa band is observed in both mouse and rat skeletal muscle lysates accompanied by an 18 kDa band in mouse Skeletal Msuscle. In mouse the protein is known as p24. The 18 kDa band could reprsent a smaller isoform.
Publications
Read Publication using NBP1-52105.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DCTN3 Antibody

  • DCTN22DCTN-22
  • dynactin 3 (p22)
  • dynactin 3
  • Dynactin complex subunit 22 kDa subunit
  • dynactin light chain
  • dynactin subunit 3
  • MGC111190
  • p22

Background

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DCTN3 Antibody (NBP1-52105)(1)

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DCTN3
Entrez
Uniprot