Nox4 Antibody [DyLight 550]


There are currently no images for Nox4 Antibody (NB110-58849R).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Pm, Rb, ShSpecies Glossary
Applications WB, IHC
Please see the vial label for concentration. If unlisted please contact technical services.

Nox4 Antibody [DyLight 550] Summary

A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 550
DyLight 550 (A=562, E=576)
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


  • Western Blot 2 ug/ml
  • Immunohistochemistry 5 ug/ml
Application Notes
This NOX4 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. Immunohistochemistry-Frozen was reported in scientific literature. In Western blot this antibody recognizes a band at ~67 kDa or larger representing isoform 1 of NOX4, and what appears to be a non-specific band ~48 kDa. The observed band size may vary depending on sample type and glycosylation. In ICC/IF cytoplasmic and mitochondrial staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human, mouse, rat, bovine, sheep, primate and rabbit (see customer review). Procine reactivity reported in scientific literature (PMID: 24403605)

Alternate Names for Nox4 Antibody [DyLight 550]

  • EC 1.6.3
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX
  • KOX-1
  • KOX-1Renal NAD(P)H-oxidase
  • NADPH oxidase 4
  • RENOXEC 1.6.3.-


NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which complex with CYBA/p22phox for generating superoxide intracellularly and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatases inhibition and function as oxygen sensor regulating the KCNK3/TASK-1 potassium channel as well as HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF- beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather thanO2 and this difference in the species generated underlie NOX-specific actions in cell signaling. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, and cardiovascular and renal complications of diabetes and in remodeling of pulmonary arteries in hypertension.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IP
Species: Hu, Mu, Po
Applications: WB, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, ICC
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Hu
Applications: WB, IHC, ICC
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb, Sh
Applications: WB, IHC

Publications for Nox4 Antibody (NB110-58849R) (0)

There are no publications for Nox4 Antibody (NB110-58849R).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Nox4 Antibody (NB110-58849R) (0)

There are no reviews for Nox4 Antibody (NB110-58849R). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Nox4 Antibody (NB110-58849R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NB110-58849B
DyLight 488 Labeled NB110-58849G
DyLight 650 Labeled NB110-58849C
HRP Labeled NB110-58849H

Additional Nox4 Antibody [DyLight 550] Products

Related Products by Gene

Bioinformatics Tool for Nox4 Antibody (NB110-58849R)

Discover related pathways, diseases and genes to Nox4 Antibody (NB110-58849R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nox4 Antibody (NB110-58849R)

Discover more about diseases related to Nox4 Antibody (NB110-58849R).

Pathways for Nox4 Antibody (NB110-58849R)

View related products by pathway.

PTMs for Nox4 Antibody (NB110-58849R)

Learn more about PTMs related to Nox4 Antibody (NB110-58849R).

Research Areas for Nox4 Antibody (NB110-58849R)

Find related products by research area.

Blogs on Nox4.

NOX4 Antibodies: Don't NOX them until you've tried them
NOX4 is an NADPH oxidase that generates superoxide within the cell. It is primarily found in vascular cells, fibroblasts, and osteoclasts, with abundant expression in the kidney. Unlike its family members NOX1 and NOX2, NOX4 is constitutively active,...  Read full blog post.

NOX4 Antibodies in Diabetic Nephropathy Research
Diabetic nephropathy (DN) is one of the leading complications resulting from chronic diabetes. It manifests as progressive renal failure caused by mesangial cell hyperplasia and fibrosis, and is one of the leading causes of terminal kidney disease (1)...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NOX4

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-58849R below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Nox4 Peptide