NOD2 Antibody (2D9) - Azide and BSA Free

Western Blot: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-NOD2 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunohistochemistry: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review. Image from the standard format of this antibody.

Flow Cytometry: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were directly conjugated to phycoerythrin.

Western Blot: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunohistochemistry: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - Analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review. Image from the standard format of this antibody.

Flow Cytometry: NOD2 Antibody (2D9) - Azide and BSA Free [NBP2-80883] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Image from the standard format of this antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
• Clone: 2D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NOD2 Antibody (2D9) - Azide and BSA Free Summary

• Immunogen: Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
• Localization: Cytoplasmic
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence : 1:50-1:500
  • Immunohistochemistry : 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin : 1:10-1:500
  • Immunoprecipitation : 1:10-1:500
  • Western Blot : 1:100-1:2000
• Application Notes: This NOD2 (2D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot. In WB, this antibody has been tested with NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature (PMID: 28241127).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NOD2 Antibody (2D9) - Azide and BSA Free

• BLAU
• CARD15caspase recruitment domain protein 15
• caspase recruitment domain family, member 15
• Caspase recruitment domain-containing protein 15
• CDACUG
• CLR16.3
• IBD1NLR family, CARD domain containing 2
• Inflammatory bowel disease protein 1
• NLRC2
• NOD-like receptor C2
• nucleotide-binding oligomerization domain 2
• nucleotide-binding oligomerization domain containing 2
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
• nucleotide-binding oligomerization domain-containing protein 2
• PSORAS1NOD2B

Background

The NOD2 (nucleotide-binding oligomerization domain containing 2) protein is expressed in the antigen-presenting cells (monocytes and macrophages) and in intestinal Paneth cells, keratinocytes, T cells, NK cells, and CD34+ stem cells. Alongwith Apaf-1 and CIITA, NOD2 belongs to NLR/NOD leucine-rich repeat containing protein family. NOD2 generally localizes to the cytoplasm from where it translocates to cell membranes, a mechanism suggested as important for its functionality. After interacting with CARD-CARD, NOD2 may bind/activate RIP2/RICK and this active NOD2:RIP2 complex then stimulates MAPK (JNK, ERK, p38), NF-kB, autophagy and antigen presentation. NOD2 has an ability to senses the presence of MDP (muramyl dipeptide), a breakdown product of peptidoglycan found in bacteria, and after recognition of intracellular bacteria, NOD2 pathway execute directing the secretion of antimicrobial peptides and cytokines/chemokines to induce innate immune clearance of the pathogen as well as to tailor the adaptive immune system to help fight the bacterial infection. Defects in NOD2 are the cause of Blau syndrome (BS), susceptibility to inflammatory bowel disease type 1 (IBD1) and sarcoidosis early-onset (EOS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NOD2 Antibody (NBP2-80883). (Showing 1 - 1 of 1 FAQ).

1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
   • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Bioinformatics

• Gene Symbol: NOD2