NOD2 Antibody (2D9) [HRP]

• Reactivity: Hu, Mu, Pm
• Applications: WB, IHC, IHC-P
• Clone: 2D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

NOD2 Antibody (2D9) [HRP] Summary

• Immunogen: Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
• Localization: Cytoplasmic
• Predicted Species: Primate (92%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This NOD2 (2D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot. In WB, this antibody has been tested with NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates (endogenous detection has some background) where a band is seen at ~110 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NOD2 Antibody (2D9) [HRP]

• BLAU
• CARD15caspase recruitment domain protein 15
• caspase recruitment domain family, member 15
• Caspase recruitment domain-containing protein 15
• CDACUG
• CLR16.3
• IBD1NLR family, CARD domain containing 2
• Inflammatory bowel disease protein 1
• NLRC2
• NOD-like receptor C2
• nucleotide-binding oligomerization domain 2
• nucleotide-binding oligomerization domain containing 2
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
• nucleotide-binding oligomerization domain-containing protein 2
• PSORAS1NOD2B

Background

NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NOD2 Antibody (NB100-524H). (Showing 1 - 1 of 1 FAQ).

1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
   • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Bioinformatics

• Gene Symbol: NOD2