NOD1 Antibody (2A10)

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 2A10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

NOD1 Antibody (2A10) Summary

• Immunogen: GST-tagged CARD domain (aa1-126) of Nod1 expressed in bacteria
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NOD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for NOD1 Antibody (2A10)

• CARD4
• CARD4NLR family, CARD domain containing 1
• caspase recruitment domain family, member 4
• Caspase recruitment domain-containing protein 4
• CLR7.1
• NLRC1
• NOD1
• nucleotide-binding oligomerization domain containing 1
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1
• nucleotide-binding oligomerization domain-containing protein 1

Background

The monoclonal antibody 2A10 recognizes human Nod1. The innate immune system is the first line of defense against pathogens using various pattern recognition receptors (PRR). PRRs include toll-like receptors (TLRs), nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible (RIG)-I-like receptors (RLR). The Nod-like receptor (NLR) family of proteins consists of cytosolic pattern recognition molecules involved in sensing microbial and danger signals and triggering innate immune activation. The founding members of NLRs are Nod1 and Nod2. These intracellular receptors recognize peptidoglycans. The receptor consists of three main domains. The C-terminal site recognizes the ligand via the leucin-rich repeat domain (LRR). The NOD domain facilitates the selfoligomerization and contains the ATPase activity. The N-terminal site is the caspase-recruitement domain (CARD). Proteoglycans are polymers composed of glycan chains of alternating N-acylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) units cross-linked to each other by short peptides. The cross-linking of two parallel glycan chains is mediated by stem peptides that can be further linked by bridging amino acids. For activation of the immune system, Nod2 senses muramyl dipeptide (MDP), which is found in the PGN of nearly all Gram-positive and Gram-negative organisms, whereas Nod1 recognizes meso-diaminopimelic acid (meso-DAP)-containing PGN fragments. Stimulation of Nod1 or Nod2 results in the activation of NF-kappaB and MAPKs, through a homophilic interaction with the adaptor kinase, Rip2. Thereby driving the transcription of numerous genes involved in both innate and adaptive immune responses. Nod1 is widely expressed in many cell types and organs. Nod1 is associated with a susceptibility to inflammatory diseases, including asthma and atopic eczema and allergic diseases

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOD1