Recombinant Human NOD1 Protein

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Recombinant Human NOD1 Protein [H00010392-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA, AP

Order Details

Recombinant Human NOD1 Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-953 of Human NOD1 full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:MEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCYPERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHPLVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAYARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTHSPVETLHAGRDTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPAAALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIYETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTYLGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKNSKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSLARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF

Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
NOD1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Theoretical MW
134.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
H00010392-P01 in the following application:

Publications
Read Publications using H00010392-P01.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human NOD1 Protein

  • CARD4
  • CARD4NLR family, CARD domain containing 1
  • caspase recruitment domain family, member 4
  • Caspase recruitment domain-containing protein 4
  • CLR7.1
  • NLRC1
  • NOD1
  • nucleotide-binding oligomerization domain containing 1
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 1
  • nucleotide-binding oligomerization domain-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for NOD1 Recombinant Protein (H00010392-P01)(3)

Review for NOD1 Recombinant Protein (H00010392-P01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00010392-P01:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 06/21/2010
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Summary

ApplicationWestern Blot
Sample Testedpurified protein, Sample Amount: 1ug
SpeciesHuman
Lot0990427
CommentsWas detected fine with GST antibody, but had some trouble with my NOD1 antibody. Was active in binding assay. NOD1 specific antibody did not work on this protein - GST tag antibody worked fine. The protein worked fine in a subsequent binding assay.

Blocking

Blocking DetailsBlocking Buffer: BLOK-CH (Millipore), Blocking Time: 30 seconds (in SNAPid system), Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: wash (Tris/NaCl/Tween), Incubation Time: 10 min, Incubation Temp: 25 C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit HRP, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#Bio-Rad 170-6515

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 20 seconds, Specific Bands: 100kd kDa, Non-Specific Bands: 40kd kDa

Comments

CommentsWas detected fine with GST antibody, but had some trouble with my NOD1 antibody. Was active in binding assay. NOD1 specific antibody did not work on this protein - GST tag antibody worked fine. The protein worked fine in a subsequent binding assay.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NOD1 Recombinant Protein (H00010392-P01) (0)

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Bioinformatics Tool for NOD1 Recombinant Protein (H00010392-P01)

Discover related pathways, diseases and genes to NOD1 Recombinant Protein (H00010392-P01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on NOD1.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

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Recent Reviews

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Anonymous
06/21/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOD1