APAF-1 Antibody


Western Blot: APAF-1 Antibody [NBP1-77000] - Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody [NBP1-77000] - Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/mL.
Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-77000] - Staining of Heart tissue at 1 ug/ml.
Western Blot: APAF-1 Antibody [NBP1-77000] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-Apaf1 at 1:1000 dilution.
Western Blot: APAF-1 Antibody [NBP1-77000] - K562 cell lysate with Apaf1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1 mg/ml

Order Details

APAF-1 Antibody Summary

Antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 - 2 mg/ml. (Optimal dilution should be determined by user. ) Whole cell lysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should be detected.
Control Peptide
APAF-1 Peptide (NBP1-77000PEP)
Read Publications using NBP1-77000.

Reactivity Notes

Human, Mouse, Rat

Alternate Names for APAF-1 Antibody

  • APAF1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APAF-1 Antibody (NBP1-77000)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for APAF-1 Antibody (NBP1-77000) (0)

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Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for APAF-1 Antibody (NBP1-77000)

Discover related pathways, diseases and genes to APAF-1 Antibody (NBP1-77000). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for APAF-1 Antibody (NBP1-77000)

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Research Areas for APAF-1 Antibody (NBP1-77000)

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Blogs on APAF-1.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol APAF1

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