Pancreatic Polypeptide/PP Antibody

Images

 
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - (5 ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. Image courtesy of anonymous customer product review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. Image from a confirmed customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer Ph9, HRP-staining.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pancreatic Polypeptide/PP Antibody Summary

Immunogen
Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 3 ug/ml
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 35-38 kDa band in human pancreas lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa band according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human pancreas show strong cytoplasmic staining of rare cells located at the periphery of islets of langerhans. An anonymous customer has reported positive results in on mouse pancreas.
Control Peptide
Pancreatic Polypeptide/PP Peptide (NB100-1793PEP)
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-1793 in the following applications:

Publications
Read Publications using
NB100-1793 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pancreatic Polypeptide/PP Antibody

  • INN
  • Obinepitide
  • pancreatic polypeptide (ppy)
  • pancreatic polypeptide PNPPP
  • pancreatic polypeptide Y
  • Pancreatic Polypeptide
  • pancreatic prohormone
  • PNP
  • PP
  • PPY

Background

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pancreatic Polypeptide/PP Antibody (NB100-1793)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
IB
(1)
ICC/IF
(2)
IHC
(5)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-1793 Applications Species
Lu TT, Heyne S, Dror E et al. The Polycomb-Dependent Epigenome Controls b Cell Dysfunction, Dedifferentiation, and Diabetes Cell Metab. Jun 5 2018 [PMID: 29754954] (IHC, Mouse) IHC Mouse
Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB) IHC, IB
Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human) IHC-Fr Human
Lee J, Sugiyama T, Liu Y et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife. 2013 Jan 1 [PMID: 24252877] (IHC-Fr, Human) IHC-Fr Human
Sugiyama T, Benitez CM, Ghodasara A et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc Natl Acad Sci U S A 2013 Jul 30 [PMID: 23852729] (IHC, Mouse) IHC Mouse
Qian J, Block GD, Colwell CS, Matveyenko AV. Consequences of exposure to light at night on the pancreatic islet circadian clock and function in rats. Diabetes 2013 Jun 17 [PMID: 23775768]
Freeby M, Ichise M, Harris PE. Vesicular monoamine transporter, type 2 (vmat2) expression as it compares to insulin and pancreatic polypeptide in the head, body and tail of the human pancreas Islets 2012 Nov 1 [PMID: 23221614] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Ono H, Yanagihara K, Sakamoto H et al. Prostate stem cell antigen gene is expressed in islets of pancreas Anat Cell Biol 2012 Sep [PMID: 23094202] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Desgraz R, Herrera PL. Pancreatic neurogenin 3-expressing cells are unipotent islet precursors. Development;136(21):3567-74. 2009 Nov. [PMID: 19793886]
Saisho Y, Harris PE, Butler AE et al. Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas. J Mol Histol. 2008 Oct [PMID: 18791800] (IHC-P, Human) IHC-P Human
Show All 11 Publications.

Reviews for Pancreatic Polypeptide/PP Antibody (NB100-1793) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-1793:
Filter by Applications
IHC-P
(2)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Pancreatic Polypeptide/PP NB100-1793
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reviewed by:
Yves Heremans
IHC-P Mouse 04/28/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
Immunohistochemistry-Paraffin Pancreatic Polypeptide/PP NB100-1793
Enlarge
reviewed by:
Anony- mous
IHC-P 12/18/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
CommentsI have tested this pancreatic polypeptide antiserum successfully in adult macaque pancreas paraffin sections following standard protocols. Please see the attached image where PP is labelled in green, and insulin is labelled in red for reference.
Immunohistochemistry-Frozen Pancreatic Polypeptide/PP NB100-1793
Enlarge
reviewed by:
Jonghyeob Lee
IHC-Fr Mouse 08/21/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Adult Pancreas Tissue
SpeciesMouse
LotP1
CommentsUsed 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Blocking

Blocking DetailsBlocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: goat-specific-HRP, Secondary Ab Dilution: 1:1000

Details

Detection NotesDetection Method: TSA-Cy5, Negative Control: no primary Ab
Fixation DetailsSample Preparation: OCT embedded Cryosection

Comments

CommentsUsed 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pancreatic Polypeptide/PP Antibody (NB100-1793) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Yves Heremans
04/28/2016
Application: IHC-P
Species: Mouse

Anony- mous
12/18/2013
Application: IHC-P
Species:

Jonghyeob Lee
08/21/2010
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PPY
Entrez
Uniprot