Pancreatic Polypeptide/PP Antibody

Immunocytochemistry/Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology and cyto-architecture compared to littermates with endogenous mouse Ins 1 and Ins 2 (top); pancreatic polypeptide (green; B,E). Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-40768-3) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Sorted CD133+ cells originate from pancreatic ducts.(A) Schematic diagram of the experimental procedure. Dissociated pancreatic cells were embedded & cultured as previously described (Lawson et al., 2007). Scale bar, 200 µm. (B) Confocal images of CD133 (green) & CPA1 (red) co-staining in adult human pancreas tissue. Scale bar, 20 µm. (C) CEL expression profiles of FACS-sorted human adult pancreatic cells & isolated islets (islet values normalized to 1). Data are presented as mean ± SEM (n=3). (D) Representative immunostaining pictures of sorted cells. Scale bar, 50 µm.DOI://dx.doi.org/10.7554/eLife.00940.005 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252877), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type & Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology & cyto-architecture compared to littermates with endogenous mouse Ins 1 & Ins 2 (top); insulin (red A–F), glucagon (green; A,D), pancreatic polypeptide (green; B,E), & somatostatin (green; C,F). G, H: Beta cell ultra-structure of NOD & Seriola dumerili Ins2 transgenic. Both NOD (G) & Seriola dumerili Ins 2 transgenic (H) islets contain insulin granules (yellow arrow), though Seriola dumerili Ins2 transgenic insulin granules are lighter in staining intensity compared to the NOD (n = 4; zoom in; inset). (I,J) Insulitis scoring pancreata from 12–15 weeks old NOD & Seriola dumerili Ins2 transgenics (n = 4 per group). Scale bar: 100 um. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30899071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Ca
• Applications: ELISA, Flow, ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pancreatic Polypeptide/PP Antibody Summary

• Immunogen: Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).
• Control Peptide:

  Pancreatic Polypeptide/PP Peptide (NB100-1793PEP)

Reactivity Notes

Monkey and Mouse reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pancreatic Polypeptide/PP Antibody

• INN
• Obinepitide
• pancreatic polypeptide (ppy)
• pancreatic polypeptide PNPPP
• pancreatic polypeptide Y
• Pancreatic Polypeptide
• pancreatic prohormone
• PNP
• PP
• PPY

Background

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pancreatic Polypeptide/PP Antibody (NB100-1793)(20)

Publications using NB100-1793

• Wang S The cell autonomous and cell non-automous roles of YAP1 during pancreatogenesis Thesis 2023-01-01
• Tixi W, Maldonado M, Chang YT et al. Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation eLife 2023-08-23 [PMID: 37610090]
• van Gurp L, Fodoulian L, Oropeza D et al. Generation of human islet cell type-specific identity genesets Nature communications [PMID: 35440614] (IHC-P, Human)
• Rovira M, Maestro Ma, Grau V, Ferrer J Hnf1b-CreER causes efficient recombination of a Rosa26-RFP reporter in duct and islet delta cells Islets 2021-07-20 [PMID: 34282714]
• Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism 2021-05-01 [PMID: 34081913] (Human)
• Bastidas-Ponce A, Tritschler S, Dony L et Al. Comprehensive single cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis Development 2020-06-23 [PMID: 31160421]
• Foo KS, Skowronski AA, Baum D et al. Transgenic substitution with Greater Amberjack Seriola dumerili fish insulin 2 in NOD mice reduces beta cell immunogenicity Sci Rep 2019-03-21 [PMID: 30899071] (IHC-P, Mouse)
• Hara A, Nakagawa Y, Nakao K et al. Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide Endocr. J. 2019-03-06 [PMID: 30842364] (ICC/IF, Human)
• Lu TT, Heyne S, Dror E et al. The Polycomb-Dependent Epigenome Controls b Cell Dysfunction, Dedifferentiation, and Diabetes Cell Metab. 2018-06-05 [PMID: 29754954] (IF/IHC, Mouse)
• Hull RL, Gibson RL, McNamara S et al. Islet Interleukin-1beta Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to beta-Cell Failure. Diabetes Care 2018-04-01 [PMID: 29437698] (Human)
• Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun 2016-08-30 [PMID: 27572106] (IF/IHC, IB)
• Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015-12-29 [PMID: 26713822] (IHC-Fr, Human)
• Lee J, Sugiyama T, Liu Y et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife. 2013-01-01 [PMID: 24252877] (IHC-Fr, Human)
• Sugiyama T, Benitez CM, Ghodasara A et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc Natl Acad Sci U S A 2013-07-30 [PMID: 23852729] (IF/IHC, Mouse)
• Qian J, Block GD, Colwell CS, Matveyenko AV. Consequences of exposure to light at night on the pancreatic islet circadian clock and function in rats. Diabetes 2013-06-17 [PMID: 23775768]
• Freeby M, Ichise M, Harris PE. Vesicular monoamine transporter, type 2 (vmat2) expression as it compares to insulin and pancreatic polypeptide in the head, body and tail of the human pancreas Islets 2012-11-01 [PMID: 23221614] (IF/IHC, ICC/IF, Human)
• Ono H, Yanagihara K, Sakamoto H et al. Prostate stem cell antigen gene is expressed in islets of pancreas Anat Cell Biol 2012-09-01 [PMID: 23094202] (ICC/IF, IF/IHC, Human)
• Desgraz R, Herrera PL. Pancreatic neurogenin 3-expressing cells are unipotent islet precursors. Development;136(21):3567-74. 2009-11-01 [PMID: 19793886]
• Saisho Y, Harris PE, Butler AE et al. Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas. J Mol Histol. 2008-10-01 [PMID: 18791800] (IHC-P, Human)
• Badman MK, Flier JS. The gut and energy balance: visceral allies in the obesity wars. Science 2005-03-25 [PMID: 15790843]

Reviews for Pancreatic Polypeptide/PP Antibody (NB100-1793) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Yves Heremans IHC-P Mouse 04/28/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse

reviewed by: Bryan Tinsley IHC-P 12/18/2013

Summary

• Application: Immunohistochemistry-Paraffin

reviewed by: Jonghyeob Lee IHC-Fr Mouse 08/21/2010

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Adult Pancreas Tissue
• Species: Mouse
• Lot: P1

Bioinformatics

• Gene Symbol: PPY
• Entrez:
  • 5539(Human)
• Uniprot:
  • P01298()
  • NP_002713.1(Human)