Pancreatic Polypeptide/PP Antibody

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - (5 ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - IHC analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. Image courtesy of anonymous customer product review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. Image from a confirmed customer review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer Ph9, HRP-staining.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pancreatic Polypeptide/PP Antibody Summary

• Immunogen: Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 35-38 kDa band in human pancreas lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa band according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human pancreas show strong cytoplasmic staining of rare cells located at the periphery of islets of langerhans. An anonymous customer has reported positive results in on mouse pancreas.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pancreatic Polypeptide/PP Antibody

• INN
• Obinepitide
• pancreatic polypeptide (ppy)
• pancreatic polypeptide PNPPP
• pancreatic polypeptide Y
• Pancreatic Polypeptide
• pancreatic prohormone
• PNP
• PP
• PPY

Background

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pancreatic Polypeptide/PP Antibody (NB100-1793)(10)

Publications using NB100-1793

• Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB)
• Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human)
• Lee J, Sugiyama T, Liu Y et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife. 2013 Jan 1 [PMID: 24252877] (IHC-Fr, Human)
• Sugiyama T, Benitez CM, Ghodasara A et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc Natl Acad Sci U S A 2013 Jul 30 [PMID: 23852729] (IHC, Mouse)
• Qian J, Block GD, Colwell CS, Matveyenko AV. Consequences of exposure to light at night on the pancreatic islet circadian clock and function in rats. Diabetes 2013 Jun 17 [PMID: 23775768]
• Freeby M, Ichise M, Harris PE. Vesicular monoamine transporter, type 2 (vmat2) expression as it compares to insulin and pancreatic polypeptide in the head, body and tail of the human pancreas Islets 2012 Nov 1 [PMID: 23221614] (IHC, ICC/IF, Human)
• Ono H, Yanagihara K, Sakamoto H et al. Prostate stem cell antigen gene is expressed in islets of pancreas Anat Cell Biol 2012 Sep [PMID: 23094202] (IHC, ICC/IF, Human)
• Desgraz R, Herrera PL. Pancreatic neurogenin 3-expressing cells are unipotent islet precursors. Development;136(21):3567-74. 2009 Nov. [PMID: 19793886]
• Saisho Y, Harris PE, Butler AE et al. Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas. J Mol Histol. 2008 Oct [PMID: 18791800] (IHC-P, Human)
• Badman MK, Flier JS. The gut and energy balance: visceral allies in the obesity wars. Science 2005 Mar 25 [PMID: 15790843]

Reviews for Pancreatic Polypeptide/PP Antibody (NB100-1793) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Yves Heremans IHC-P Mouse 04/28/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse

reviewed by: Anony- mous IHC-P 12/18/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Comments: I have tested this pancreatic polypeptide antiserum successfully in adult macaque pancreas paraffin sections following standard protocols. Please see the attached image where PP is labelled in green, and insulin is labelled in red for reference.

reviewed by: Jonghyeob Lee IHC-Fr Mouse 08/21/2010

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Adult Pancreas Tissue
• Species: Mouse
• Lot: P1
• Comments: Used 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Blocking

• Blocking Details: Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: goat-specific-HRP, Secondary Ab Dilution: 1:1000

Details

• Detection Notes: Detection Method: TSA-Cy5, Negative Control: no primary Ab
• Fixation Details: Sample Preparation: OCT embedded Cryosection

Comments

• Comments: Used 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Bioinformatics

• Gene Symbol: PPY
• Entrez:
  • 5539(Human)
• Uniprot:
  • P01298()