WB: Preliminary experiments gave an approx. 35-38 kDa band in human pancreas lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa band according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human pancreas show strong cytoplasmic staining of rare cells located at the periphery of islets of langerhans. An anonymous customer has reported positive results in on mouse pancreas.
Predicted cross-reactivity based on sequence identity: Rat, Canine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Pancreatic Polypeptide/PP Antibody
pancreatic polypeptide (ppy)
pancreatic polypeptide PNPPP
pancreatic polypeptide Y
Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
I have tested this pancreatic polypeptide antiserum successfully in adult macaque pancreas paraffin sections following standard protocols. Please see the attached image where PP is labelled in green, and insulin is labelled in red for reference.
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