Pancreatic Polypeptide/PP Antibody

Immunocytochemistry/Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology and cyto-architecture compared to littermates with endogenous mouse Ins 1 and Ins 2 (top); pancreatic polypeptide (green; B,E). Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-40768-3) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Pm, Ca
• Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Pancreatic Polypeptide/PP Antibody Summary

• Immunogen: Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPY
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).
• Control Peptide:

  Pancreatic Polypeptide/PP Peptide (NB100-1793PEP)

Reactivity Notes

Monkey and Mouse reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pancreatic Polypeptide/PP Antibody

• INN
• Obinepitide
• pancreatic polypeptide (ppy)
• pancreatic polypeptide PNPPP
• pancreatic polypeptide Y
• Pancreatic Polypeptide
• pancreatic prohormone
• PNP
• PP
• PPY

Background

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pancreatic Polypeptide/PP Antibody (NB100-1793)(14)

Publications using NB100-1793

• Foo KS, Skowronski AA, Baum D et al. Transgenic substitution with Greater Amberjack Seriola dumerili fish insulin 2 in NOD mice reduces beta cell immunogenicity Sci Rep Mar 21 2019 [PMID: 30899071] (IHC-P, Mouse)
• Hara A, Nakagawa Y, Nakao K et al. Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide Endocr. J. Mar 6 2019 [PMID: 30842364] (ICC/IF, Human)
• Lu TT, Heyne S, Dror E et al. The Polycomb-Dependent Epigenome Controls b Cell Dysfunction, Dedifferentiation, and Diabetes Cell Metab. Jun 5 2018 [PMID: 29754954] (IHC, Mouse)
• Hull RL, Gibson RL, McNamara S et al. Islet Interleukin-1beta Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to beta-Cell Failure. Diabetes Care 2018 Apr [PMID: 29437698] (Human)
• Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB)
• Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human)
• Lee J, Sugiyama T, Liu Y et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife. 2013 Jan 1 [PMID: 24252877] (IHC-Fr, Human)
• Sugiyama T, Benitez CM, Ghodasara A et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc Natl Acad Sci U S A 2013 Jul 30 [PMID: 23852729] (IHC, Mouse)
• Qian J, Block GD, Colwell CS, Matveyenko AV. Consequences of exposure to light at night on the pancreatic islet circadian clock and function in rats. Diabetes 2013 Jun 17 [PMID: 23775768]
• Freeby M, Ichise M, Harris PE. Vesicular monoamine transporter, type 2 (vmat2) expression as it compares to insulin and pancreatic polypeptide in the head, body and tail of the human pancreas Islets 2012 Nov 1 [PMID: 23221614] (IHC, ICC/IF, Human)
• Ono H, Yanagihara K, Sakamoto H et al. Prostate stem cell antigen gene is expressed in islets of pancreas Anat Cell Biol 2012 Sep [PMID: 23094202] (IHC, ICC/IF, Human)
• Desgraz R, Herrera PL. Pancreatic neurogenin 3-expressing cells are unipotent islet precursors. Development;136(21):3567-74. 2009 Nov. [PMID: 19793886]
• Saisho Y, Harris PE, Butler AE et al. Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas. J Mol Histol. 2008 Oct [PMID: 18791800] (IHC-P, Human)
• Badman MK, Flier JS. The gut and energy balance: visceral allies in the obesity wars. Science 2005 Mar 25 [PMID: 15790843]

Reviews for Pancreatic Polypeptide/PP Antibody (NB100-1793) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Yves Heremans IHC-P Mouse 04/28/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse

reviewed by: Anony- mous IHC-P 12/18/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Comments: I have tested this pancreatic polypeptide antiserum successfully in adult macaque pancreas paraffin sections following standard protocols. Please see the attached image where PP is labelled in green, and insulin is labelled in red for reference.

reviewed by: Jonghyeob Lee IHC-Fr Mouse 08/21/2010

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Adult Pancreas Tissue
• Species: Mouse
• Lot: P1
• Comments: Used 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Blocking

• Blocking Details: Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:500, Primary Ab Incubation Time: 1 hour, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: goat-specific-HRP, Secondary Ab Dilution: 1:1000

Details

• Detection Notes: Detection Method: TSA-Cy5, Negative Control: no primary Ab
• Fixation Details: Sample Preparation: OCT embedded Cryosection

Comments

• Comments: Used 1:500 (even 1:2500 worked) on mouse adult pancreas. anti-goat-HRP 1:1000 followed by TSA-Cy5 amplification (Perkin Elmer). Worked quite well.

Bioinformatics

• Gene Symbol: PPY
• Entrez:
  • 5539(Human)
• Uniprot:
  • P01298()
  • NP_002713.1(Human)