Pancreatic Polypeptide/PP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet ...read more
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, ...read more
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Adult macaque pancrease paraffin sections following standard protocols. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, CaSpecies Glossary
Applications Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pancreatic Polypeptide/PP Antibody Summary

Immunogen
Peptide with sequence C-TRPRYGKRHKEDT corresponding to internal region according to NP_002713.1.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23221614). Use in immunoblotting reported in scientific literature (PMID: 27572106).
Control Peptide
Pancreatic Polypeptide/PP Peptide (NB100-1793PEP)
Reviewed Applications
Read 3 Reviews rated 5
using
NB100-1793 in the following applications:

Publications
Read Publications using
NB100-1793 in the following applications:

Reactivity Notes

Monkey and Mouse reactivity reported from verified customer reviews.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pancreatic Polypeptide/PP Antibody

  • INN
  • Obinepitide
  • pancreatic polypeptide (ppy)
  • pancreatic polypeptide PNPPP
  • pancreatic polypeptide Y
  • Pancreatic Polypeptide
  • pancreatic prohormone
  • PNP
  • PP
  • PPY

Background

Gene ID: 5539 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
NBP2-37447
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
NBP1-46535
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
NBP1-05167
Species: Hu
Applications: ELISA, IHC, IHC-P, ELISA(Cap), S-ELISA
NBP2-62664
Species: Hu
Applications: IHC, IHC-P
NBP3-12223
Species: Hu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP2-46349
Species: Hu
Applications: IHC, WB
MAB8864
Species: Hu
Applications: IHC
H00008767-M02
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP2-48988
Species: Hu
Applications: IHC, IHC-P
NBP2-33903
Species: Hu
Applications: IHC, IHC-P
NBP2-93868
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP3-03282
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87404
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
NB100-1533
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
MAB4077
Species: Hu
Applications: IHC, WB
MAB4375
Species: Hu, Mu, Rt
Applications: IHC

Publications for Pancreatic Polypeptide/PP Antibody (NB100-1793)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
IB
(1)
ICC/IF
(3)
IHC
(5)
IHC-Fr
(2)
IHC-P
(3)
All Applications
Filter By Species
Human
(9)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-1793 Applications Species
van Gurp L, Fodoulian L, Oropeza D et al. Generation of human islet cell type-specific identity genesets Nature communications [PMID: 35440614] (IHC-P, Human) IHC-P Human
Rovira M, Maestro Ma, Grau V, Ferrer J Hnf1b-CreER causes efficient recombination of a Rosa26-RFP reporter in duct and islet delta cells Islets Jul 20 2021 [PMID: 34282714]
Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism May 1 2021 [PMID: 34081913] (Human) Human
Foo KS, Skowronski AA, Baum D et al. Transgenic substitution with Greater Amberjack Seriola dumerili fish insulin 2 in NOD mice reduces beta cell immunogenicity Sci Rep Mar 21 2019 [PMID: 30899071] (IHC-P, Mouse) IHC-P Mouse
Hara A, Nakagawa Y, Nakao K et al. Development of monoclonal mouse antibodies that specifically recognize pancreatic polypeptide Endocr. J. Mar 6 2019 [PMID: 30842364] (ICC/IF, Human) ICC/IF Human
Lu TT, Heyne S, Dror E et al. The Polycomb-Dependent Epigenome Controls b Cell Dysfunction, Dedifferentiation, and Diabetes Cell Metab. Jun 5 2018 [PMID: 29754954] (IHC, Mouse) IHC Mouse
Hull RL, Gibson RL, McNamara S et al. Islet Interleukin-1beta Immunoreactivity Is an Early Feature of Cystic Fibrosis That May Contribute to beta-Cell Failure. Diabetes Care 2018 Apr [PMID: 29437698] (Human) Human
Kim-Muller Jy, Fan J, Kim Yj et al. Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic beta cells in diabetic mice. Nat Commun Aug 30 2016 [PMID: 27572106] (IHC, IB) IHC, IB
Cinti F, Bouchi R, Kim-Muller JY et al. Evidence of B-cell Dedifferentiation in Human Type 2 Diabetes. J. Clin. Endocrinol. Metab. 2015 Dec 29 [PMID: 26713822] (IHC-Fr, Human) IHC-Fr Human
Lee J, Sugiyama T, Liu Y et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. Elife. 2013 Jan 1 [PMID: 24252877] (IHC-Fr, Human) IHC-Fr Human
Show All 17 Publications.

Reviews for Pancreatic Polypeptide/PP Antibody (NB100-1793) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-1793:
Filter by Applications
IHC-P
(2)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Pancreatic Polypeptide/PP NB100-1793
Enlarge
5
reviewed by:
Verified Customer
IHC-P Mouse 04/28/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
Immunohistochemistry-Paraffin Pancreatic Polypeptide/PP NB100-1793
Enlarge
5
reviewed by:
Verified Customer
IHC-P 12/18/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Immunohistochemistry-Frozen Pancreatic Polypeptide/PP NB100-1793
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 08/21/2010
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Adult Pancreas Tissue
SpeciesMouse
LotP1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Pancreatic Polypeptide/PP Antibody (NB100-1793) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Pancreatic Polypeptide/PP Products

Bioinformatics Tool for Pancreatic Polypeptide/PP Antibody (NB100-1793)

Discover related pathways, diseases and genes to Pancreatic Polypeptide/PP Antibody (NB100-1793). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Pancreatic Polypeptide/PP Antibody (NB100-1793)

Discover more about diseases related to Pancreatic Polypeptide/PP Antibody (NB100-1793).
 

Pathways for Pancreatic Polypeptide/PP Antibody (NB100-1793)

View related products by pathway.

PTMs for Pancreatic Polypeptide/PP Antibody (NB100-1793)

Learn more about PTMs related to Pancreatic Polypeptide/PP Antibody (NB100-1793).
 

Research Areas for Pancreatic Polypeptide/PP Antibody (NB100-1793)

Find related products by research area.

Blogs on Pancreatic Polypeptide/PP

There are no specific blogs for Pancreatic Polypeptide/PP, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
04/28/2016
Application: IHC-P
Species: Mouse

Verified Customer
12/18/2013
Application: IHC-P
Species:

Verified Customer
08/21/2010
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol PPY
Entrez
Uniprot