NKX2.2 Antibody (CL11380) - BSA Free

Staining of human Liver shows no positivity in hepatocytes as expected.

Analysis in human cell line U-138MG.

Staining of human Stomach shows strong nuclear positivity in enteroendocrine cells.

Staining of human Pancreas shows strong nuclear positivity in islets of Langerhans.

Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Analysis in human cerebral cortex and liver tissues using NBP3-21191 antibody. Corresponding NKX2-2 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL11380
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NKX2.2 Antibody (CL11380) - BSA Free Summary

• Description: Novus Biologicals Mouse NKX2.2 Antibody (CL11380) - BSA Free (NBP3-21191) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O95096, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (96%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NKX2.2 Antibody (CL11380) - BSA Free

• Homeobox protein NK-2 homolog B
• NK-2 (Drosophila) homolog B
• NK2 homeobox 2
• NK2 transcription factor related, locus 2 (Drosophila)
• NK2 transcription factor related, locus 2
• NK2 transcription factor-like protein B
• NKX2.2
• NKX2.2homeobox protein Nkx-2.2
• NKX2B
• NKX2BNK-2 homolog B

Background

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-2