NG2/MCSP Antibody

Images

 
Western Blot: NG2/MCSP Antibody [NBP1-21361] - NG2 Antibody [NBP1-21361] - Whole cell lysate from HeLa cells. MCSP was also immunoprecipitated by rabbit anti-MCSP antibody NBP1-21362.
Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-21361] - NG2 Antibody [NBP1-21361] - Primary cultured trabecular meshwork cells from porcine eye. Left non stretched TM cells; Right 24 hours stretched TM ...read more

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

NG2/MCSP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human melanoma-associated chondroitin sulfate proteoglycan (chondroitin sulfate proteoglycan 4 ) using the numbering given in entry NP_001888.2 (GeneID 1464).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSPG4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 5-10 ug/mg lysate
Application Notes
Success in ICC stain of pig samples has also been reported by customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-21361 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

The porcine reactivity was reported by an anonymous customer.

Alternate Names for NG2/MCSP Antibody

  • AN2
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
  • chondroitin sulfate proteoglycan 4
  • CSPG4
  • EC 3.6.3
  • HMW-MAA
  • MCSP
  • MCSPChondroitin sulfate proteoglycan NG2
  • MCSPG
  • MCSPGMelanoma chondroitin sulfate proteoglycan
  • MEL-CSPG
  • MSK16Melanoma-associated chondroitin sulfate proteoglycan
  • NG2
  • NG2EC 2.7.8

Background

Melanoma chondroitin sulfate proteoglycan (MCSP) is a cell-surface antigen expressed early in melanoma progression. It has been found to be involved in the spreading, invasion, and migration of human melanoma. MCSP influences melanoma behavior via the activation of the Cdc42 GTPase and phosphorylation of p130Cas. MCSP also activates signaling cascades involving FAK and ERK1/ERK2. In addition to its expression in melanoma cells, MCSP is also found in breast carcinomas, gliomas, neuroblastomas, and acute leukemias. MCSP is being considered as a target antigen for cancer immunotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NG2/MCSP Antibody (NBP1-21361) (0)

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Review for NG2/MCSP Antibody (NBP1-21361) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-21361:
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Images Ratings Applications Species Date Details
Immunocytochemistry NG2/MCSP NBP1-21361
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reviewed by:
Corey.
ICC Other 10/19/2011
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Summary

ApplicationImmunocytochemistry
Sample TestedPrimary cultured trabecular meshwork cells from pig eye
SpeciesOther
LotA1

Blocking

Blocking DetailsBlocking Buffer: 5% NGS NDS

Primary Anitbody

Dilution RatioPrimary Ab Incubation Dilution Buffer: PBS, Primary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Donkey anti-rabbit Alexa 488, Secondary Ab Dilution: 1:1000

Details

Fixation DetailsSample Preparation: 2% PA 10 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NG2/MCSP Antibody (NBP1-21361) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Corey.
10/19/2011
Application: ICC
Species: Other

Bioinformatics

Gene Symbol CSPG4
Entrez
Uniprot