NG2/MCSP Antibody

Western Blot: NG2/MCSP Antibody [NBP1-21361] - NG2 Antibody [NBP1-21361] - Whole cell lysate from HeLa cells. MCSP was also immunoprecipitated by rabbit anti-MCSP antibody NBP1-21362.

Immunocytochemistry/Immunofluorescence: NG2/MCSP Antibody [NBP1-21361] - NG2 Antibody [NBP1-21361] - Primary cultured trabecular meshwork cells from porcine eye. Left non stretched TM cells; Right 24 hours stretched TM cells. Annonymous Customer Reported Findings.

• Reactivity: Hu, Po
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NG2/MCSP Antibody Summary

• Description: The porcine reactivity was reported by an anonymous customer.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human melanoma-associated chondroitin sulfate proteoglycan (chondroitin sulfate proteoglycan 4 ) using the numbering given in entry NP_001888.2 (GeneID 1464).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSPG4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Validated from a verified customer review
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

The porcine reactivity was reported by an anonymous customer.

Alternate Names for NG2/MCSP Antibody

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

Melanoma chondroitin sulfate proteoglycan (MCSP) is a cell-surface antigen expressed early in melanoma progression. It has been found to be involved in the spreading, invasion, and migration of human melanoma. MCSP influences melanoma behavior via the activation of the Cdc42 GTPase and phosphorylation of p130Cas. MCSP also activates signaling cascades involving FAK and ERK1/ERK2. In addition to its expression in melanoma cells, MCSP is also found in breast carcinomas, gliomas, neuroblastomas, and acute leukemias. MCSP is being considered as a target antigen for cancer immunotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for NG2/MCSP Antibody (NBP1-21361) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Corey . ICC Other 10/19/2011

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary cultured trabecular meshwork cells from pig eye
• Species: Other
• Lot: A1

Bioinformatics

• Gene Symbol: CSPG4
• Entrez:
  • 1464(Human)
• Uniprot:
  • Q6UVK1()