Reactivity | Hu, Mu, Rt, Po, Ch, Fe, Gp, Ma, Pm, RbSpecies Glossary |
Applications | Flow |
Clone | NE14 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Allophycocyanin |
Immunogen | Crude neurofilament preparation from porcine spinal cord |
Localization | Cytoplasmic |
Marker | Neuronal Marker |
Specificity | This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | NEFH |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NBP2-47834AF350 | |
Alexa Fluor 405 | NBP2-47834AF405 | |
Alexa Fluor 488 | NBP2-47834AF488 | |
Alexa Fluor 532 | NBP2-47834AF532 | |
Alexa Fluor 594 | NBP2-47834AF594 | |
Alexa Fluor 647 | NBP2-47834AF647 | |
Alexa Fluor 700 | NBP2-47834AF700 | |
Alexa Fluor 750 | NBP2-47834AF750 | |
Allophycocyanin | NBP2-47834APC | |
Biotin | NBP2-47834B | |
DyLight 350 | NBP2-47834UV | |
DyLight 405 | NBP2-47834V | |
DyLight 488 | NBP2-47834G | |
DyLight 550 | NBP2-47834R | |
DyLight 594 | NBP2-47834DL594 | |
DyLight 650 | NBP2-47834C | |
DyLight 680 | NBP2-47834FR | |
DyLight 755 | NBP2-47834IR | |
FITC | NBP2-47834F | |
HRP | NBP2-47834H | |
Janelia Fluor 549 | NBP2-47834JF549 | |
Janelia Fluor 646 | NBP2-47834JF646 | |
PE | NBP2-47834PE | |
PerCP | NBP2-47834PCP |
Diseases for NF-H Antibody (NBP2-47834APC)Discover more about diseases related to NF-H Antibody (NBP2-47834APC).
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PTMs for NF-H Antibody (NBP2-47834APC)Learn more about PTMs related to NF-H Antibody (NBP2-47834APC).
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Gene Symbol | NEFH |