Tau Antibody (2E9)


Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] ...read more
Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained ...read more
Immunocytochemistry/ Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - E18 primary rat hippocampal neurons (from Neuromics) were fixed and immunostained with NBP2-25162 (green), chicken antibody to alpha Internexin ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, Func, ICC/IF, IHC
1 mg/ml

Order Details

Tau Antibody (2E9) Summary

Recombinant full length 441 amino acid human tau. Epitope located in the peptide KDRVQSKIGSLDNITHVPGG, amino acids 347-366.
Cytoplasm > cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm > cytoskeleton. Cell projection > axon. Note: Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
pan-Neuronal Marker
This antibody is expected to bind to all Tau isoforms
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Proximity Ligation Assay
  • Western Blot 1:10000
Application Notes
Use in PLA reported in scientific literature (PMID:35264729) Optimal dilution of this antibody should be experimentally determined..
Theoretical MW
48-67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-25162 in the following applications:

  • IHC
    1 publication
  • PLA
    1 publication

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% PBS, 50% glycerol
5mM Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for Tau Antibody (2E9)

  • FLJ31424
  • FTDP-17
  • G protein beta1/gamma2 subunit-interacting factor 1
  • MAPT
  • MGC138549
  • microtubule-associated protein tau
  • MSTD
  • MTBT1
  • MTBT1Neurofibrillary tangle protein
  • MTBT2
  • Neurofibrillary tangle protein
  • PHF-tau
  • PPND
  • Tau
  • TAUPaired helical filament-tau


Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them. This early work showed that the Tau protein facilitated the polymerization of microtubules, and was therefore named after the Greek letter tau, since it promoted tubule formation. Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non-neuronal cells. Much interest has focused on Tau as it is a major component of the neurofibrillary tangles of Alzheimer's disease. Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the Tau gene are causative of Frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). There is one mammalian Tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range in size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called "big tau" predominates, another alternate transcript which includes a 254 amino acid insert. This form of Tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa. Each Tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of "intrinsically unstructured proteins". As with GAP43, MARCKS and several other similar proteins, Tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight. This antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various Tau proteins, so this antibody is expected to bind to all Tau isoforms.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, S-ELISA, WB
Species: Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Ca, Ch, ChHa, Fe, Hu, Mu, Ma-Op, Po, Pm, Rt
Applications: ICC/IF, Simple Western, WB
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Av, Bv, Sh
Applications: WB
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
Species: Ba, Pp, Hu, I, Pm, Mu, Rt
Applications: ELISA, Func, ICC/IF, IHC, IHC-P, IP, KD, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, Func, ICC/IF, IHC

Publications for Tau Antibody (NBP2-25162)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, PLA.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for Tau Antibody (NBP2-25162) (0)

There are no reviews for Tau Antibody (NBP2-25162). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tau Antibody (NBP2-25162) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Tau Products

Diseases for Tau Antibody (NBP2-25162)

Discover more about diseases related to Tau Antibody (NBP2-25162).

Pathways for Tau Antibody (NBP2-25162)

View related products by pathway.

PTMs for Tau Antibody (NBP2-25162)

Learn more about PTMs related to Tau Antibody (NBP2-25162).

Research Areas for Tau Antibody (NBP2-25162)

Find related products by research area.

Blogs on Tau.

HIV-associated neurocognitive disorders involve extracellular Nef-induced modification of lipid rafts and redistribution of Alzheimer’s disease-related proteins
Jamshed Arslan, Pharm D, PhD Cholesterol is an essential part of animal cell membranes. Cholesterol-rich lipid rafts maintain the fluidity and protein trafficking of plasma membranes. Cellular ABCA1 protein moves cho...  Read full blog post.

The C99 fragment of amyloid precursor protein (APP)
Alzheimer’s Disease (AD) is a neurodegenerative disorder that is characterized by an abundance of the beta-amyloid peptide in the brain.  When AD was first discovered, it was determined that beta-amyloid was produced as a result of the prote...  Read full blog post.

Tau - A microtubule associated protein as a biomarker for Alzheimer's disease
The tau protein is a microtubule associated protein found mostly in neuronal cells where it regulates the stability of axonal microtubules as well as kinesin-dependent transport. Tau is relevant in the study of various neurological disorders as ab...  Read full blog post.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

New Study Links Tau Mutations to Microglial Immune Response
Tau proteins are abundant in the axons of neurons in the central nervous system (CNS), and play a key role in microtubule formation and stabilization. Antibody studies have identified six tau isoforms, all produced by alternative mRNA splicing of the ...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Tau Antibody (2E9) and receive a gift card or discount.


Gene Symbol MAPT