NF-H Antibody (NE14) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Ge, GP, Pm, RbSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
NE14
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

NF-H Antibody (NE14) [Janelia Fluor® 646] Summary

Immunogen
Crude neurofilament preparation from porcine spinal cord
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
This MAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEFH
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Alternate Names for NF-H Antibody (NE14) [Janelia Fluor® 646]

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47834AF350
Alexa Fluor 405 NBP2-47834AF405
Alexa Fluor 488 NBP2-47834AF488
Alexa Fluor 532 NBP2-47834AF532
Alexa Fluor 594 NBP2-47834AF594
Alexa Fluor 647 NBP2-47834AF647
Alexa Fluor 700 NBP2-47834AF700
Alexa Fluor 750 NBP2-47834AF750
Allophycocyanin NBP2-47834APC
Allophycocyanin/Cy7 NBP2-47834APCCY7
Biotin NBP2-47834B
DyLight 350 NBP2-47834UV
DyLight 405 NBP2-47834V
DyLight 488 NBP2-47834G
DyLight 550 NBP2-47834R
DyLight 594 NBP2-47834DL594
DyLight 650 NBP2-47834C
DyLight 680 NBP2-47834FR
DyLight 755 NBP2-47834IR
FITC NBP2-47834F
HRP NBP2-47834H
Janelia Fluor 549 NBP2-47834JF549
Janelia Fluor 646 NBP2-47834JF646
PE NBP2-47834PE
PE/Atto594 NBP2-47834PEATT594
PE/Cy5.5 NBP2-47834PECY55
PE/Cy7 NBP2-47834PECY7
PerCP NBP2-47834PCP

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Bioinformatics Tool for NF-H Antibody (NBP2-47834JF646)

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Bioinformatics

Gene Symbol NEFH