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Cdk5 Antibody

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Western Blot: Cdk5 Antibody [NBP2-15843] - Wild-type (WT) and CDK5 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500. ...read more
Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP2-15843] - Analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-15843] - Rat hind brain. CDK5 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Cdk5 Antibody [NBP2-15843] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK5 antibody diluted at 1:500.
Western Blot: Cdk5 Antibody [NBP2-15843] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE diluted at 1:1000
Western Blot: Cdk5 Antibody [NBP2-15843] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Immunohistochemistry-Paraffin: Cdk5 Antibody [NBP2-15843] - ES2 xenograft, using CDK5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cdk5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK5. The exact sequence is proprietary.
Localization
Cytoplasm; Cell projection; lamellipodium; growth cone
Predicted Species
Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDK5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Cdk5 Antibody

  • CDK5
  • CDKN5
  • Cell division protein kinase 5
  • cyclin-dependent kinase 5
  • EC 2.7.11
  • EC 2.7.11.22
  • LIS7
  • protein kinase CDK5 splicing
  • PSSALRE
  • Serine/threonine-protein kinase PSSALRE
  • Tau protein kinase II catalytic subunit
  • TPKII catalytic subunit

Background

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDK5
Uniprot