Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: ELA10-101.5 (39A)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free Summary

• Description: Novus Biologicals Mouse Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free (NB100-64392) is a monoclonal antibody validated for use in ELISA and Flow. Anti-Neutrophil Elastase/ELA2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Elastase from human neutrophils
• Localization: Secreted
• Specificity: NB100-64392 is specific for human neutrophil elastase.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELANE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) - BSA Free

• Bone marrow serine protease
• EC 3.4.21
• EC 3.4.21.37
• ELA2
• ELA2granulocyte-derived elastase
• ELANE
• elastase 2, neutrophil
• elastase, neutrophil expressed
• Elastase-2
• GE
• HLEelastase-2
• HNE
• Human leukocyte elastase
• Leukocyte Elastase
• Medullasin
• NE
• Neutrophil Elastase
• PMN elastase
• PMN-E
• polymorphonuclear elastase
• SCN1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neutrophil Elastase/ELA2 Antibody (NB100-64392)(2)

Publications using NB100-64392

• Davies PL, Maxwell NC, Kotecha S, Spiller OB. Monoclonal anti-neutrophil elastase antibody characterisation: Ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules. J Immunol Methods. 2008-01-01 [PMID: 18504046]
• Durant, S et al. Apoptosis-induced proteinase 3 membrane expression is independent from degranulation. J Leukoc Biol 75: 87-98. 2004-01-01 [PMID: 14525959]

Bioinformatics

• Gene Symbol: ELANE
• Entrez:
  • 1991(Human)
• Uniprot:
  • P08246()
  • P08246()