Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) [Alexa Fluor® 488]

Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [Alexa Fluor® 488] [NB100-64392AF488] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review.

• Reactivity: Hu
• Applications: ELISA, Flow
• Clone: ELA10-101.5 (39A)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) [Alexa Fluor® 488] Summary

• Immunogen: Elastase from human neutrophils
• Localization: Secreted
• Specificity: NB100-64392 is specific for human neutrophil elastase.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ELANE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50 mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Neutrophil Elastase/ELA2 Antibody (ELA10-101.5 (39A)) [Alexa Fluor® 488]

• Bone marrow serine protease
• EC 3.4.21
• EC 3.4.21.37
• ELA2
• ELA2granulocyte-derived elastase
• ELANE
• elastase 2, neutrophil
• elastase, neutrophil expressed
• Elastase-2
• GE
• HLEelastase-2
• HNE
• Human leukocyte elastase
• Leukocyte Elastase
• Medullasin
• NE
• Neutrophil Elastase
• PMN elastase
• PMN-E
• polymorphonuclear elastase
• SCN1

Background

The ELANE gene (commonly known as neutrophil elastase) encodes a 267 amino acid long, 28 kDA neutrophil elastase protein that functions in regulation of natural killer cells, monocytes, and graulocytes. ELANE is involved in activation of proMMP8, cell adhesion cell-matrix glycoconjugates, selected targets of C/EBPbeta, degradation of the extracellular matrix and collagen, and amb2 integrin signaling. It interacts with various genes GZMB, LRP1, SERPINF2, SERPING1, and SERPINA1. Elane has been linked to cystic fibrosis, asthma, pneumonia, alzheimer's disease, neutropenia, cutis laxia, leg ulcers, wegener's granulomatosis, bronchitis, adult respiratory distress syndrome, and vascular diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Neutrophil Elastase/ELA2 Antibody (NB100-64392AF488) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Vincent Giacalone Flow Human 05/27/2020

Summary

• Application: Flow Cytometry
• Sample Tested: BAL fluid
• Species: Human
• Lot: 170810-080718

Comments

• Comments: I used this antibody to stain for surface-bound neutrophil elastase. This enzyme is released from neutrophils following exocytosis of the primary granules and can be captured on the surface of neutrophils and macrophages, but less so on lymphocytes. I purified cells from the BAL of cystic fibrosis patients and stained for analysis by flow cytometry. Macrophages were found to have large amounts of surface-bound elastase, while it is minimal on the T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations.

Bioinformatics

• Gene Symbol: ELANE