Nephrin Antibody

Images

 
Western Blot: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody [NBP1-77303] - Mouse Kidney cells.
Immunohistochemistry: Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue with Nephrin antibody at 1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Nephrin Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPHS1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
Application Notes
This antibody can be used for detection of Nephrin by Western blot at 0.5 - 1 mg/ml.
Control Peptide
Nephrin Peptide (NBP1-77303PEP)
Publications
Read Publications using
NBP1-77303 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nephrin Antibody

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (NBP1-77303)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPHS1
Entrez
Uniprot