Western Blot: Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated ...read more
Immunohistochemistry: Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.
Western Blot: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.
Western Blot: Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue with Nephrin antibody at 1 ug/ml.
Immunofluorescence: Nephrin Antibody [NBP1-77303] - Rat kidney tissue with Nephrin antibody at 20 ug/mL.
Immunofluorescence: Nephrin Antibody [NBP1-77303] - Mouse Kidney cells with Nephrin antibody at 10 ug/mL.
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. Amino Acid Sequence: EPDSLPFELRGHLV
Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). NBP1-77303 can detect isoforms of human, mouse and rat.
Peptide affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for Nephrin Antibody
nephrosis 1, congenital, Finnish type (nephrin)
Renal glomerulus-specific cell adhesion receptor
Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Nephrin Antibody (NBP1-77303). (Showing 1 - 1 of 1 FAQs).
I was reviewing the datasheet for product, NBP177303 (anti-Nephrin Ab) and I did not see any antigen retrieval information. Could you please let me know what is required for antigen retrieval?
There are no special instructions for this antibody and, therefore, none are recommended on the website. We recommend that you proceed with your usual protocol. Here is a link to our general protocols and they just need to be optimized. http://www.novusbio.com/support/support-by-application/immunohistochemistry-paraffin/protocol.html
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.