Nephrin Antibody


Western Blot: Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated more
Immunohistochemistry: Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.
Western Blot: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.
Western Blot: Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.
Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue with Nephrin antibody at 1 ug/ml.
Immunofluorescence: Nephrin Antibody [NBP1-77303] - Rat kidney tissue with Nephrin antibody at 20 ug/mL.
Immunofluorescence: Nephrin Antibody [NBP1-77303] - Mouse Kidney cells with Nephrin antibody at 10 ug/mL.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF
1 mg/ml

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Nephrin Antibody Summary

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. Amino Acid Sequence: EPDSLPFELRGHLV
Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). NBP1-77303 can detect isoforms of human, mouse and rat.
Peptide affinity purified
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  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Immunofluorescence 10 ug/ml
Application Notes
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Nephrin Knockout HeLa Cell Lysate
Control Peptide
Nephrin Peptide (NBP1-77303PEP)
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NBP1-77303 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Peptide affinity purified

Alternate Names for Nephrin Antibody

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor


Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (NBP1-77303)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nephrin Antibody (NBP1-77303). (Showing 1 - 1 of 1 FAQs).

  1. I was reviewing the datasheet for product, NBP177303 (anti-Nephrin Ab) and I did not see any antigen retrieval information. Could you please let me know what is required for antigen retrieval?
    • There are no special instructions for this antibody and, therefore, none are recommended on the website. We recommend that you proceed with your usual protocol. Here is a link to our general protocols and they just need to be optimized.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol NPHS1