Podocalyxin Like Antibody

Images

 
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated ...read more
Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Podocalyxin Like Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1556 in the following applications:

Publications
Read Publications using
AF1556 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podocalyxin Like Antibody (AF1556)(37)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(5)
IHC
(16)
IHC-Fr
(9)
IHC-P
(5)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(34)
Rat
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using AF1556 Applications Species
IS Zaitoun, YS Song, HB Zaitoun, CM Sorenson, N Sheibani Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice Cells, 2022-10-21;11(20):. 2022-10-21 [PMID: 36291198] (IHC, Mouse) IHC Mouse
H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain Science Advances, 2021-12-08;7(50):eabi5464. 2021-12-08 [PMID: 34878843] (IHC, Mouse) IHC Mouse
K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021-09-29;10(0):. 2021-09-29 [PMID: 34586063] (IHC, Mouse) IHC Mouse
T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease Scientific Reports, 2021-06-24;11(1):13260. 2021-06-24 [PMID: 34168254] (IHC, Mouse) IHC Mouse
C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy Sci Adv, 2020-05-27;6(22):eaba4542. 2020-05-27 [PMID: 32518827] (IHC, Mouse) IHC Mouse
M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome Nat Commun, 2020-03-12;11(1):1343. 2020-03-12 [PMID: 32165640] (ICC, Mouse) ICC Mouse
RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling Sci Rep, 2019-10-11;9(1):14677. 2019-10-11 [PMID: 31605007] (IHC-P, Mouse) IHC-P Mouse
M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco Acute and chronic hypoxia differentially predispose lungs for metastases Sci Rep, 2019-07-15;9(1):10246. 2019-07-15 [PMID: 31308473] (IHC-Fr, Mouse) IHC-Fr Mouse
I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander Evaporative cooling provides a major metabolic energy sink Mol Metab, 2019-07-01;0(0):. 2019-07-01 [PMID: 31302039] (IHC-P, Mouse) IHC-P Mouse
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019-06-27;10(1):2817. 2019-06-27 [PMID: 31249304] (IHC, Mouse) IHC Mouse
Show All 37 Publications.

Reviews for Podocalyxin Like Antibody (AF1556) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using AF1556:
Filter by Applications
IHC-P
(1)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
IHC-P Rat 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24100472
SpeciesRat
  5
reviewed by:
Anonymous -
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23338608
SpeciesMouse
  4
reviewed by:
Anonymous -
ICC Human 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24306541
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous -
01/05/2015
Application: IHC-P
Species: Rat

 
Anonymous -
01/05/2015
Application: IF
Species: Mouse

 
Anonymous -
01/05/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PODXL
Uniprot