Podocalyxin Like Antibody [Unconjugated]

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Defects in TRAF3IP1 mutants are mediated by MAP4.(a) Lateral views of WT zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in WT embryos injected with control or map4 morpholino. (b) Lateral views of elipsa zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in elipsa mutant embryos injected with control or map4 morpholino (data shown as combined result of n=3 independent experiments). Scale bars, 1 mm. (c) Relative expression of Map4 normalized to that of Hprt was analysed by qPCR in control and Traf3ip1-KD mIMCD3 cells stably expressing GFP or GFP-IFT54 mutants and Map4 shRNA. (d) Control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions were fixed in MeOH and stained for acetylated alpha -tubulin (red) and gamma -tubulin (light blue). Scale bar, 10 μm. (e) Six hours after Ca2+ switch, mIMCD3 cells grown until confluence on filters were fixed with 4% PFA and stained for the apical marker Gp135 (red). Scale bar, 10 μm. (f) Percentage of normal spheroids of control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions grown on Matrigel for 5 days (mean ± s.d., n≥100 spheroids from 3 independent experiments, ***P≤0,0001, *P4-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

TRAF3IP1 mutations lead to epithelialization and polarity defects.(a) mIMCD3 cells grown until confluence on filters were subjected to Ca2+-free medium to disrupt the tight junctions. Six hours after Ca2+ addition, cells were analysed by immunofluorescence using the apical marker Gp135 (red) and beta -catenin (light blue) to stain the cell junctions. Scale bar, 10 μm. (b) Following Ca2+ switch, tight junction re-formation was assessed by measurement of trans-epithelial resistance (TER) at different time points (mean ± s.e.m. of n=5 independent experiments, two-way ANOVA; NS: not-significant, ***P<0.001 at 6 h). (c) Height of mIMCD3 cells grown on filters measured as the distance from the base to the top of the cells (GFP staining, not shown; mean ± s.d. of n≥20, from 3 independent experiments, ***P<0.001, Bonferonni's multiple-comparison test). (d) Expression of the apical marker Gp135 was analysed by Western blot with alpha -tubulin as a loading control. (e) mIMCD3 cells grown in matrigel 3D matrix to form spheroids were stained for ZO1 (tight junctions, red) and analysed by confocal microscopy. Arrows indicate ZO-1 at the apical junctions, while arrow heads point to mislocalized ZO-1. Equatorial sections of representative spheres are shown for each cell line. Scale bars, 10 μm. (f) Percentage of abnormal spheroids (no/small lumen filled with cells) (mean ± s.d., n=80 spheroids from 2 independent experiments, ***P≤0,001, **P<0.002, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26487268), licensed under a CC-BY license. Not internally tested by R&D Systems.

Improved survival&blood–brain barrier function post pneumococcal infection in mice by anti-HIF-1 alpha treatment using echinomycin. e Immunofluorescence staining for HIF-1 alpha , BBB permeability&junctional markers in the echinomycin&vehicle groups at the survival end point. Left panel shows that echinomycin treatment leads to a reduction in HIF-1 alpha -positive nuclei including in ECs co-stained for podocalyxin, a vascular marker which was unchanged by the treatment. Middle panel displays reduced vascular permeability to fibrinogen in the echinomycin group as indicated by stronger intravascular signal compared to the vehicle-treated mice. Increased expression of tight junction proteins—occludin (middle panel)&claudin-5 (right top panel)—in echinomycin-treated mice indicate improved BBB function. Tight junction-associated ZO-1, adherens junction marker VE-Cadherin,&endothelial cell adhesion molecule CD31 unchanged (middle, bottom right panel). There was also no difference in S. pneumoniae (Spn) staining (right top panel) between the two groups. Scale bar 10 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32529267), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Podocalyxin Like Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
  Ser21-Arg402
  Accession # Q9R0M4
• Specificity: Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODXL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody [Unconjugated]

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

1. Li, J. et al. (2001) DNA Seq. 12(5):407.
2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (AF1556)(104)

Publications using AF1556

• Torrente, D;Su, E;Citalán?Madrid, A;Schielke, G;Magaoay, D;Warnock, M;Stevenson, T;Mann, K;Lesept, F;Delétage, N;Blanc, M;Norris, E;Vivien, D;Lawrence, D; The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in ?-synuclein-mediated neurotoxicity Journal of neuroinflammation 2025-01-14 [PMID: 39810216] (IHC-F, Mouse)
• Cybulsky, AV;Papillon, J;Guillemette, J;Navarro-Betancourt, JR;Elimam, H;Fantus, IG; Genetic deletion of calcium-independent phospholipase A2? protects mice from diabetic nephropathy PloS one 2024-10-31 [PMID: 39480824] (Western Blot, Mouse)
• Panzade, G;Srivastava, T;Heruth, DP;Rezaiekhaligh, MH;Zhou, J;Lyu, Z;Sharma, M;Joshi, T; Employing Multi-Omics Analyses to Understand Changes during Kidney Development in Perinatal Interleukin-6 Animal Model Cells 2024-10-09 [PMID: 39404429] (Immunohistochemistry, Mouse)
• Liu, J;Xiong, Y;Mo, H;Niu, H;Miao, J;Shen, W;Zhou, S;Wang, X;Li, X;Zhang, Y;Ma, K;Zhou, L; MicroRNA-29b Plays a Vital Role in Podocyte Injury and Glomerular Diseases through Inducing Mitochondrial Dysfunction International journal of biological sciences 2024-09-03 [PMID: 39309435] (Immunohistochemistry, Western Blot, Human)
• Janes, PW;Parslow, AC;Cao, D;Rigopoulos, A;Lee, FT;Gong, SJ;Cartwright, GA;Burvenich, IJG;Eriksson, U;Johns, TG;Scott, FE;Scott, AM; An Anti-VEGF-B Antibody Reduces Abnormal Tumor Vasculature and Enhances the Effects of Chemotherapy Cancers 2024-05-16 [PMID: 38791979] (Immunohistochemistry, Xenograft)
• Niasse, A;Louis, K;Lenoir, O;Schwarz, C;Xu, X;Couturier, A;Dobosziewicz, H;Corchia, A;Placier, S;Vandermeersch, S;Hennighausen, L;Frère, P;Galichon, P;Surin, B;Ouchelouche, S;Louedec, L;Migeon, T;Verpont, MC;Yousfi, N;Buob, D;Xu-Dubois, YC;François, H;Rondeau, E;Mesnard, L;Hadchouel, J;Luque, Y; Protective Role of the Podocyte IL-15 / STAT5 Pathway in Focal Segmental Glomerulosclerosis Kidney international reports 2024-04-01 [PMID: 38765560] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Moritz Schanz, Martin Kimmel, Mark Dominik Alscher, Kerstin Amann, Christoph Daniel TIMP-2 and IGFBP7 in human kidney biopsies in renal disease Clinical Kidney Journal 2023-08-31 [PMID: 37664566]
• Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA; Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction Journal of the American Heart Association 2023-06-22 [PMID: 37345826] (Immunohistochemistry, Transgenic Mouse)
• Chen, Y;Gong, C;Chauhan, S;De La Cruz, ED;Datta, MS;Tomer, R; Scalable projected Light Sheet Microscopy for high-resolution imaging of large samples bioRxiv : the preprint server for biology 2023-06-05 [PMID: 37333196] (Immunohistochemistry, Mouse)
• Chen, X;Wolfe, DA;Bindu, DS;Zhang, M;Taskin, N;Goertsen, D;Shay, TF;Sullivan, EE;Huang, SF;Ravindra Kumar, S;Arokiaraj, CM;Plattner, VM;Campos, LJ;Mich, JK;Monet, D;Ngo, V;Ding, X;Omstead, V;Weed, N;Bishaw, Y;Gore, BB;Lein, ES;Akrami, A;Miller, C;Levi, BP;Keller, A;Ting, JT;Fox, AS;Eroglu, C;Gradinaru, V; Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates Nature communications 2023-06-08 [PMID: 37291094] (IHC, Mouse)
• Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong et al. Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse Frontiers in Cell and Developmental Biology 2023-05-05 [PMID: 37215091]
• Satu Paavonsalo, Yelin Subashi, Madeleine H. Lackman, Sinem Karaman Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine STAR Protocols 2023-06-01 [PMID: 37182207]
• Xinhong Chen, Damien A. Wolfe, Dhanesh Sivadasan Sivadasan Bindu, Mengying Zhang, Naz Taskin, David Goertsen et al. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. bioRxiv 2023-01-18 [PMID: 36711773] (Immunohistochemistry, Mouse)
• Stobart JL, Erlebach E, Gl�ck C et al. Altered hemodynamics and vascular reactivity in a mouse model with severe pericyte deficiency Journal of Cerebral Blood Flow & Metabolism 2023-05-01 [PMID: 36545806]
• IS Zaitoun, YS Song, HB Zaitoun, CM Sorenson, N Sheibani Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice Cells, 2022-10-21;11(20):. 2022-10-21 [PMID: 36291198] (IHC, Mouse)
• Sara Gillnäs, Radiosa Gallini, Liqun He, Christer Betsholtz, Johanna Andrae Severe cerebellar malformations in mutant mice demonstrate a role for PDGF-C/PDGFR alpha signalling in cerebellar development Biology Open 2022-08-15 [PMID: 35876806]
• Laurent Jacob, Jose de Brito Neto, Stephanie Lenck, Celine Corcy, Farhat Benbelkacem, Luiz Henrique Geraldo et al. Conserved meningeal lymphatic drainage circuits in mice and humans Journal of Experimental Medicine 2022-08-01 [PMID: 35776089]
• Chunhong Wang, Jiafeng Liu, Xiaoyao Zhang, Qiyan Chen, Xiaoyan Bai, Xue Hong et al. Role of miRNA-671-5p in Mediating Wnt/ beta -Catenin-Triggered Podocyte Injury Frontiers in Pharmacology 2022-01-17 [PMID: 35111054]
• Peter Baluk, Donald M. McDonald Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts Methods in Molecular Biology 2022-09-06 [PMID: 35099733]
• Sinem Karaman, Satu Paavonsalo, Krista Heinolainen, Madeleine H. Lackman, Amanda Ranta, Karthik A. Hemanthakumar et al. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance Journal of Experimental Medicine 2022-03-07 [PMID: 35050301]
• H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain Science Advances, 2021-12-08;7(50):eabi5464. 2021-12-08 [PMID: 34878843] (IHC, Mouse)
• J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021-11-29;12(1):6963. 11/29/2021 [PMID: 34845225]
• Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al. Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation Frontiers in Physiology 2021-09-29 [PMID: 34658910]
• K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021-09-29;10(0):. 2021-09-29 [PMID: 34586063] (IHC, Mouse)
• Logan T, Simon MJ, Rana A Et al. Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologic Cell 8/25/2021 [PMID: 34450028]
• Belicova L, Repnik U, Delpierre J et al. Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads Journal of Cell Biology 2021-10-04 [PMID: 34328499]
• Belicova L, Repnik U, Delpierre J et al. Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads Journal of Cell Biology 10/4/2021 [PMID: 34328499]
• Johanna Schaffenrath, Sheng-Fu Huang, Tania Wyss, Mauro Delorenzi, Annika Keller Characterization of the blood–brain barrier in genetically diverse laboratory mouse strains Fluids and Barriers of the CNS 2021-07-28 [PMID: 34321020]
• T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease Scientific Reports, 2021-06-24;11(1):13260. 2021-06-24 [PMID: 34168254] (IHC, Mouse)
• Milich LM, Choi JS, Ryan CB et al. Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cord J Exp Med 6/16/2021 [PMID: 34132743]
• Sarah Guimbal, Lauriane Cornuault, Paul Rouault, Pierre-Louis Hollier, Candice Chapouly, Marie-Lise Bats et al. Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice Arteriosclerosis, Thrombosis, and Vascular Biology 2021-04-01 [PMID: 33567863]
• José R. Navarro-Betancourt, Joan Papillon, Julie Guillemette, Takao Iwawaki, Chen-Fang Chung, Andrey V. Cybulsky Role of IRE1 alpha in podocyte proteostasis and mitochondrial health Cell Death Discovery 2020-11-19 [PMID: 33298866] (Immunohistochemistry, Transgenic Mouse)
• Bryan Marsh, Robert Blelloch Single nuclei RNA-seq of mouse placental labyrinth development eLife 2020-11-03 [PMID: 33141023]
• Fabrizio Orsenigo, Lei Liu Conze, Suvi Jauhiainen, Monica Corada, Francesca Lazzaroni, Matteo Malinverno et al. Mapping endothelial-cell diversity in cerebral cavernous malformations at single-cell resolution eLife 2020-11-03 [PMID: 33138917]
• Fadi E. Pulous, Jamie C. Carnevale, Zaki Al-Yafeai, Brenna H. Pearson, Jamie A. G. Hamilton, Curtis J. Henry et al. Talin-dependent integrin activation is required for endothelial proliferation and postnatal angiogenesis Angiogenesis 2021-02-01 [PMID: 33113074] (Immunohistochemistry, Mouse)
• Helene Rundqvist, Pedro Veliça, Laura Barbieri, Paulo A Gameiro, David Bargiela, Milos Gojkovic et al. Cytotoxic T-cells mediate exercise-induced reductions in tumor growth eLife 2020-10-23 [PMID: 33095157]
• Amanda J. Eakin, Tamara Mc Erlain, Aileen Burke, Amy Eaton, Nuala Tipping, Gloria Allocca et al. Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function Frontiers in Cell and Developmental Biology 2020-08-19 [PMID: 32974345]
• Devraj, G, GuErit, S Et al. HIF-1 alpha is involved in blood-brain barrier dysfunction and paracellular migration of bacteria in pneumococcal meningitis. Acta Neuropathol 8/1/2020 [PMID: 32529267]
• C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy Sci Adv, 2020-05-27;6(22):eaba4542. 2020-05-27 [PMID: 32518827] (IHC, Mouse)
• Dan H, Ruan T, Sampogna RV Circadian Clock Regulation of Developmental Time in the Kidney Cell Rep 5/19/2020 [PMID: 32433970]
• M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome Nat Commun, 2020-03-12;11(1):1343. 2020-03-12 [PMID: 32165640] (ICC, Mouse)
• Zhenyang Yu, Jian Zeng, Jun Wang, Yaxiong Cui, Xiaopeng Song, Yizhe Zhang et al. Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability Cardiovascular Research 2021-01-21 [PMID: 32044971]
• Michaela Roth, Andreas Enström, Candice Aghabeick, Robert Carlsson, Guillem Genové, Gesine Paul Parenchymal pericytes are not the major contributor of extracellular matrix in the fibrotic scar after stroke in male mice Journal of Neuroscience Research 5/1/2020 [PMID: 31758600]
• Hanan Elimam, Joan Papillon, Julie Guillemette, José R. Navarro-Betancourt, Andrey V. Cybulsky Genetic Ablation of Calcium-independent Phospholipase A2 gamma Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice Scientific Reports 2019-11-07 [PMID: 31700134]
• RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling Sci Rep, 2019-10-11;9(1):14677. 2019-10-11 [PMID: 31605007] (IHC-P, Mouse)
• Paul A. Mueller, Liping Yang, Margo Ubele, Guogen Mao, Jason Brandon, Julia Vandra et al. The coronary artery disease risk-associated Plpp3 gene and its product lipid phosphate phosphatase 3 regulate experimental atherosclerosis Arteriosclerosis, Thrombosis, and Vascular Biology 2019-11-01 [PMID: 31533471]
• M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco Acute and chronic hypoxia differentially predispose lungs for metastases Sci Rep, 2019-07-15;9(1):10246. 2019-07-15 [PMID: 31308473] (IHC-Fr, Mouse)
• Martin Crivello, Marion C. Hogg, Elisabeth Jirström, Luise Halang, Ina Woods, Megan Rayner et al. Vascular regression precedes motor neuron loss in the FUS (1-359) ALS mouse model Disease Models & Mechanisms 2019-08-01 [PMID: 31383794]
• I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander Evaporative cooling provides a major metabolic energy sink Mol Metab, 2019-07-01;0(0):. 2019-07-01 [PMID: 31302039] (IHC-P, Mouse)
• R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019-06-27;10(1):2817. 2019-06-27 [PMID: 31249304] (IHC, Mouse)
• M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana Endothelial cell clonal expansion in the development of cerebral cavernous malformations Nat Commun, 2019-06-24;10(1):2761. 2019-06-24 [PMID: 31235698] (IHC, Transgenic Mouse)
• G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019-05-28;10(1):2350. 2019-05-28 [PMID: 31138815] (Western Blot, Human)
• D Nilsson, M Heglind, Z Arani, S Enerbäck Foxc2 is essential for podocyte function Physiol Rep, 2019-05-01;7(9):e14083. 2019-05-01 [PMID: 31062503] (IHC, Mouse)
• Benz, F;Wichitnaowarat, V;Lehmann, M;Germano, RF;Mihova, D;Macas, J;Adams, RH;Taketo, MM;Plate, KH;Guerit, S;Vanhollebeke, B;Liebner, S; Low wnt/beta-catenin signaling determines leaky vessels in the subfornical organ and affects water homeostasis in mice Elife 4/1/2019 [PMID: 30932814]
• Lili Zhou, Xiaowen Chen, Meizhi Lu, Qinyu Wu, Qian Yuan, Chengxiao Hu et al. Wnt/ beta -catenin links oxidative stress to podocyte injury and proteinuria Kidney International 2019-04-01 [PMID: 30770219]
• T. Yang, G. Xu, P.T. Newton, A.S. Chagin, S. Mkrtchian, M. Carlström et al. Maresin 1 attenuates neuroinflammation in a mouse model of perioperative neurocognitive disorders British Journal of Anaesthesia 2019-03-01 [PMID: 30770053]
• M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability Nat Commun, 2018-12-17;9(1):5357. 2018-12-17 [PMID: 30559384] (IHC, Mouse)
• Koji Okamoto, Jason W. Rausch, Hidefumi Wakashin, Yulong Fu, Joon-Yong Chung, Patrick D. Dummer et al. APOL1 risk allele RNA contributes to renal toxicity by activating protein kinase R Communications Biology 2018-11-07 [PMID: 30417125]
• M Takahashi, M Tamura, S Sato, K Kawakami Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele Dis Model Mech, 2018-10-25;0(0):. 2018-10-25 [PMID: 30237319] (IHC, Mouse)
• M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018-08-30;37(1):209. 2018-08-30 [PMID: 30165893] (IHC, Mouse)
• Mette C. Jørgensen, Kristian H. de Lichtenberg, Caitlin A. Collin, Rasmus Klinck, Jeppe H. Ekberg, Maja S. Engelstoft et al. Neurog3-dependent pancreas dysgenesis causes ectopic pancreas in Hes1 mutant mice Development 2018-01-18 [PMID: 30093553]
• Takao Hikita, Fatemeh Mirzapourshafiyi, Pedro Barbacena, Meghan Riddell, Ayesha Pasha, Mengnan Li et al. PAR ‐3 controls endothelial planar polarity and vascular inflammation under laminar flow EMBO reports 2018-09-01 [PMID: 30018153]
• TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy Sci Rep, 2018-07-10;8(1):10396. 2018-07-10 [PMID: 29991751] (IHC, Mouse)
• E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643120] (IHC-Fr, Mouse)
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Reviews for Podocalyxin Like Antibody (AF1556) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Verified Customer IHC-P Rat 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24100472
• Species: Rat

reviewed by: Verified Customer IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23338608
• Species: Mouse

reviewed by: Verified Customer ICC Human 01/05/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 24306541
• Species: Human

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • Q9R0M4()