Podocalyxin Like Antibody

Images

 
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated ...read more
Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Podocalyxin Like Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1556 in the following applications:

Publications
Read Publications using
AF1556 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Podocalyxin Like Antibody (AF1556)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(4)
IHC
(9)
IHC-Fr
(9)
IHC-P
(5)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(28)
Rat
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using AF1556 Applications Species
RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling Sci Rep, 2019;9(1):14677. 2019 [PMID: 31605007] (IHC-P, Mouse) IHC-P Mouse
M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco Acute and chronic hypoxia differentially predispose lungs for metastases Sci Rep, 2019;9(1):10246. 2019 [PMID: 31308473] (IHC-Fr, Mouse) IHC-Fr Mouse
I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander Evaporative cooling provides a major metabolic energy sink Mol Metab, 2019;0(0):. 2019 [PMID: 31302039] (IHC-P, Mouse) IHC-P Mouse
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019;10(1):2817. 2019 [PMID: 31249304] (IHC, Mouse) IHC Mouse
G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019;10(1):2350. 2019 [PMID: 31138815] (Western Blot, Human) Western Blot Human
D Nilsson, M Heglind, Z Arani, S Enerbäck Foxc2 is essential for podocyte function Physiol Rep, 2019;7(9):e14083. 2019 [PMID: 31062503] (IHC, Mouse) IHC Mouse
M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability Nat Commun, 2018;9(1):5357. 2018 [PMID: 30559384] (IHC, Mouse) IHC Mouse
M Takahashi, M Tamura, S Sato, K Kawakami Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele Dis Model Mech, 2018;0(0):. 2018 [PMID: 30237319] (IHC, Mouse) IHC Mouse
M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018;37(1):209. 2018 [PMID: 30165893] (IHC, Mouse) IHC Mouse
TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy Sci Rep, 2018;8(1):10396. 2018 [PMID: 29991751] (IHC, Mouse) IHC Mouse
Show All 30 Publications.

Reviews for Podocalyxin Like Antibody (AF1556) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using AF1556:
Filter by Applications
IHC-P
(1)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Rat 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24100472
SpeciesRat
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23338608
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23338608

Details

Detection NotesSee PMID 23338608

Comments

CommentsPublished. See PMID 23338608
 
reviewed by:
Anonymous
ICC Human 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24306541
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24306541

Details

Detection NotesSee PMID 24306541

Comments

Comments"Formulation: 0.2µm filtered solution in PBS.Reconstitution and storage: reconstitute with sterile PBS. Diluted 1:100 by using 0.5 ml PBS then divided in to 20 aliquot and stored frozen at -20ºc in manual defrost freezer for 12 months without loss of activity. Specificity: mouse podocalyxin extracellular domain,Tissue staining kit (R&D system catalog CTS008). Immunocytochemistry using enzymatic protocol (2) R&D system: "Published: PMID 24306541

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FAQs for Podocalyxin Like Antibody (AF1556) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1556

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: IHC-P
Species: Rat

 
Anonymous
01/05/2015
Application: IF
Species: Mouse

 
Anonymous
01/05/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol PODXL
Uniprot
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