Podocalyxin Like Antibody

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Podocalyxin Like Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
  Ser21-Arg402
  Accession # Q9R0M4
• Specificity: Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODXL
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody

• GCTM
• Gp200MGC138240
• PCLP
• PCLP1
• PCLP-1
• PCLPGCTM-2 antigen
• PCpodocalyxin
• POD XL
• Podocalyxin
• Podocalyxin-like protein 1
• podocalyxin-like
• PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

1. Li, J. et al. (2001) DNA Seq. 12(5):407.
2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Podocalyxin Like Antibody (AF1556)(37)

Publications using AF1556

• IS Zaitoun, YS Song, HB Zaitoun, CM Sorenson, N Sheibani Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice Cells, 2022-10-21;11(20):. 2022-10-21 [PMID: 36291198] (IHC, Mouse)
• H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain Science Advances, 2021-12-08;7(50):eabi5464. 2021-12-08 [PMID: 34878843] (IHC, Mouse)
• K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021-09-29;10(0):. 2021-09-29 [PMID: 34586063] (IHC, Mouse)
• T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease Scientific Reports, 2021-06-24;11(1):13260. 2021-06-24 [PMID: 34168254] (IHC, Mouse)
• C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy Sci Adv, 2020-05-27;6(22):eaba4542. 2020-05-27 [PMID: 32518827] (IHC, Mouse)
• M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome Nat Commun, 2020-03-12;11(1):1343. 2020-03-12 [PMID: 32165640] (ICC, Mouse)
• RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling Sci Rep, 2019-10-11;9(1):14677. 2019-10-11 [PMID: 31605007] (IHC-P, Mouse)
• M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco Acute and chronic hypoxia differentially predispose lungs for metastases Sci Rep, 2019-07-15;9(1):10246. 2019-07-15 [PMID: 31308473] (IHC-Fr, Mouse)
• I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander Evaporative cooling provides a major metabolic energy sink Mol Metab, 2019-07-01;0(0):. 2019-07-01 [PMID: 31302039] (IHC-P, Mouse)
• R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019-06-27;10(1):2817. 2019-06-27 [PMID: 31249304] (IHC, Mouse)
• M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana Endothelial cell clonal expansion in the development of cerebral cavernous malformations Nat Commun, 2019-06-24;10(1):2761. 2019-06-24 [PMID: 31235698] (IHC, Transgenic Mouse)
• G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019-05-28;10(1):2350. 2019-05-28 [PMID: 31138815] (Western Blot, Human)
• D Nilsson, M Heglind, Z Arani, S Enerbäck Foxc2 is essential for podocyte function Physiol Rep, 2019-05-01;7(9):e14083. 2019-05-01 [PMID: 31062503] (IHC, Mouse)
• M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability Nat Commun, 2018-12-17;9(1):5357. 2018-12-17 [PMID: 30559384] (IHC, Mouse)
• M Takahashi, M Tamura, S Sato, K Kawakami Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele Dis Model Mech, 2018-10-25;0(0):. 2018-10-25 [PMID: 30237319] (IHC, Mouse)
• M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer J. Exp. Clin. Cancer Res., 2018-08-30;37(1):209. 2018-08-30 [PMID: 30165893] (IHC, Mouse)
• TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy Sci Rep, 2018-07-10;8(1):10396. 2018-07-10 [PMID: 29991751] (IHC, Mouse)
• E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643120] (IHC-Fr, Mouse)
• R Hatano, A Takeda, Y Abe, K Kawaguchi, I Kazama, M Matsubara, S Asano Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice Sci Rep, 2018-03-14;8(1):4512. 2018-03-14 [PMID: 29540766] (Western Blot, Mouse)
• DO Dias, H Kim, D Holl, B Werne Soln, J Lundeberg, M Carlén, C Göritz, J Frisén Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury Cell, 2018-03-01;173(1):153-165.e22. 2018-03-01 [PMID: 29502968] (IHC-Fr, Mouse)
• M Crivello, SL O'Riordan, I Woods, S Cannon, L Halang, KS Coughlan, MC Hogg, SA Lewandowsk, JHM Prehn Pleiotropic activity of systemically delivered angiogenin in the SOD1G93Amouse model Neuropharmacology, 2018-02-25;133(0):503-511. 2018-02-25 [PMID: 29486168] (IHC-Fr, Mouse)
• K Song, J Fu, J Song, BH Herzog, K Bergstrom, Y Kondo, JM McDaniel, S McGee, R Silasi-Man, F Lupu, H Chen, H Bagavant, L Xia Loss of mucin-type O-glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney J. Biol. Chem., 2017-08-25;292(40):16491-16497. 2017-08-25 [PMID: 28842487] (IHC, Western Blot, Mouse)
• SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1 Sci Rep, 2017-07-07;7(1):4916. 2017-07-07 [PMID: 28687807] (IHC, Mouse)
• DR Kaufman, J Papillon, L Larose, T Iwawaki, AV Cybulsky Deletion of Inositol-Requiring Enzyme-1? in Podocytes Disrupts Glomerular Capillary Integrity and Autophagy Mol. Biol. Cell, 2017-04-20;0(0):. 2017-04-20 [PMID: 28428258] (IHC-P, Mouse)
• Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice Sci Rep, 2016-08-05;6(0):30739. 2016-08-05 [PMID: 27491826] (IHC-P, Mouse)
• EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016-07-29;7(0):12329. 2016-07-29 [PMID: 27470974] (IHC-Fr, Mouse)
• J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen The development and plasticity of alveolar type 1 cells Development, 2015-11-19;143(1):54-65. 2015-11-19 [PMID: 26586225] (ICC, Mouse)
• Cunha S, Bocci M, Lovrot J, Eleftheriou N, Roswall P, Cordero E, Lindstrom L, Bartoschek M, Haller B, Pearsall R, Mulivor A, Kumar R, Larsson C, Bergh J, Pietras K Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer. Cancer Res, 2015-06-15;75(12):2445-56. 2015-06-15 [PMID: 26077471] (IHC-Fr, Mouse)
• Al-Momany A, Li L, Alexander R, Ballermann B Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A. J Cell Sci, 2014-10-24;127(24):5164-78. 2014-10-24 [PMID: 25344252] (ICC, Mouse)
• Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria. Am J Pathol, 2014-06-18;184(8):2211-24. 2014-06-18 [PMID: 24951831] (ICC, Mouse, Rat)
• Lenoir O, Milon M, Virsolvy A, Henique C, Schmitt A, Masse J, Kotelevtsev Y, Yanagisawa M, Webb D, Richard S, Tharaux P Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis. J Am Soc Nephrol, 2014-04-10;25(5):1050-62. 2014-04-10 [PMID: 24722437] (IHC-P, Mouse)
• Ramasamy S, Kusumbe A, Wang L, Adams R Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature, 2014-03-12;507(7492):376-80. 2014-03-12 [PMID: 24647000] (IHC-Fr, Mouse)
• Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Development, 2013-12-18;141(2):448-59. 2013-12-18 [PMID: 24353059] (IHC, Mouse)
• Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota. Am J Pathol, 2013-10-03;183(6):1945-59. 2013-10-03 [PMID: 24096077] (IHC-Fr, Mouse)
• Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, Franco M, Paez-Ribes M, Cordiner R, Fuxe J, Johansson B, Goumans M, Casanovas O, ten Dijke P, Arthur H, Pietras K Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination. J Exp Med, 2013-02-11;210(3):563-79. 2013-02-11 [PMID: 23401487] (IHC-Fr, Mouse)
• Pierchala BA, Munoz MR, Tsui CC Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function. Kidney Int., 2010-07-21;78(9):868-82. 2010-07-21 [PMID: 20664558] (ICC, Mouse)
• Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002. 2005-05-25 [PMID: 15917337] (IHC, Rat)

Reviews for Podocalyxin Like Antibody (AF1556) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous - IHC-P Rat 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24100472
• Species: Rat

reviewed by: Anonymous - IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23338608
• Species: Mouse

reviewed by: Anonymous - ICC Human 01/05/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 24306541
• Species: Human

Bioinformatics

• Gene Symbol: PODXL
• Uniprot:
  • Q9R0M4()