Podocalyxin Like Antibody

Images

 
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated ...read more
Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Podocalyxin Like Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PODXL
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1556 in the following applications:

Publications
Read Publications using
AF1556 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocalyxin Like Antibody

  • GCTM
  • Gp200MGC138240
  • PCLP
  • PCLP1
  • PCLP-1
  • PCLPGCTM-2 antigen
  • PCpodocalyxin
  • POD XL
  • Podocalyxin
  • Podocalyxin-like protein 1
  • podocalyxin-like
  • PODXL

Background

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-45316
Species: Hu, Pm
Applications: Flow, ICC/IF, IF, IHC, IHC-P, WB
H00001719-M01
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-64775
Species: Pm, Bv, Hu, Mu, Pm
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P (-), IP
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
664-LI/CF
Species: Hu
Applications: BA
NBP3-07168
Species: Pm, Ca, Pm, Hu, Pm, Sq
Applications: Flow, ICC/IF
NBP1-89688
Species: Hu
Applications: IHC, IHC-P, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
AF5866
Species: Hu
Applications: IHC, WB
NBP2-15104
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14093
Species: Hu
Applications: IHC, IHC-P, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AF1556
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for Podocalyxin Like Antibody (AF1556)(36)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(5)
IHC
(14)
IHC-Fr
(9)
IHC-P
(5)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(33)
Rat
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF1556 Applications Species
H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain Science Advances, 2021;7(50):eabi5464. 2021 [PMID: 34878843] (IHC, Mouse) IHC Mouse
K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021;10(0):. 2021 [PMID: 34586063] (IHC, Mouse) IHC Mouse
T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease Scientific Reports, 2021;11(1):13260. 2021 [PMID: 34168254] (IHC, Mouse) IHC Mouse
C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy Sci Adv, 2020;6(22):eaba4542. 2020 [PMID: 32518827] (IHC, Mouse) IHC Mouse
M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome Nat Commun, 2020;11(1):1343. 2020 [PMID: 32165640] (ICC, Mouse) ICC Mouse
RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling Sci Rep, 2019;9(1):14677. 2019 [PMID: 31605007] (IHC-P, Mouse) IHC-P Mouse
M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco Acute and chronic hypoxia differentially predispose lungs for metastases Sci Rep, 2019;9(1):10246. 2019 [PMID: 31308473] (IHC-Fr, Mouse) IHC-Fr Mouse
I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander Evaporative cooling provides a major metabolic energy sink Mol Metab, 2019;0(0):. 2019 [PMID: 31302039] (IHC-P, Mouse) IHC-P Mouse
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019;10(1):2817. 2019 [PMID: 31249304] (IHC, Mouse) IHC Mouse
M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana Endothelial cell clonal expansion in the development of cerebral cavernous malformations Nat Commun, 2019;10(1):2761. 2019 [PMID: 31235698] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Show All 36 Publications.

Reviews for Podocalyxin Like Antibody (AF1556) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using AF1556:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
Immunofluorescence
(1)
Immunocytochemistry
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
Immunohistochemistry-Paraffin Rat 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24100472
SpeciesRat
  5
reviewed by:
Anonymous -
Immunofluorescence Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23338608
SpeciesMouse
  4
reviewed by:
Anonymous -
Immunocytochemistry Human 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24306541
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Podocalyxin Like Antibody (AF1556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Podocalyxin Like Products

Bioinformatics Tool for Podocalyxin Like Antibody (AF1556)

Discover related pathways, diseases and genes to Podocalyxin Like Antibody (AF1556). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Podocalyxin Like Antibody (AF1556)

Discover more about diseases related to Podocalyxin Like Antibody (AF1556).
 

Pathways for Podocalyxin Like Antibody (AF1556)

View related products by pathway.

PTMs for Podocalyxin Like Antibody (AF1556)

Learn more about PTMs related to Podocalyxin Like Antibody (AF1556).

Blogs on Podocalyxin Like

There are no specific blogs for Podocalyxin Like, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous -
01/05/2015
Application: Immunohistochemistry-Paraffin
Species: Rat

 
Anonymous -
01/05/2015
Application: Immunofluorescence
Species: Mouse

 
Anonymous -
01/05/2015
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol PODXL
Uniprot