Nephrin: Novus Biologicals

AF3159

Nephrin Antibody [Unconjugated]

100 μg

AF3159

NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Immunofluorescence analysis of PRCP and nephrin at 60x magnification eleven weeks after 2K1C.White arrows indicate PRCP staining. A) Localization of PRCP and nephrin with overlay in the control kidney. B) Localization of PRCP and nephrin with overlay in the unclipped kidney. C) Localization of PRCP and nephrin with overlay in the clipped kidney. D) Quantitative analysis of cortical, glomerular and tubular PRCP expression in control, unclipped and clipped kidneys. *p < 0.05 vs. Control. **p < 0.01 vs. Unclipped. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25706121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder.(a) Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localised to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 μm. (b) Confocal images of Strawberry expression in the liver, spleen, lung, urinary bladder and left and right kidney of ELS infected animals 60 days post left intrarenal injection (top panels) and uninjected control animals (bottom panels). Sections were stained with an antibody against Strawberry (green) and merged images with DAPI (blue) are presented. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26046460), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Podocyte‐specific knockout of KDM6A in mice protects against diabetes‐induced kidney injuryAQuantitative RT–PCR evaluation of KDM6A mRNA expression in kidney glomeruli isolated from wild‐type and KDM6A‐knockout (KO) mice. *P < 0.05, significant difference versus wild‐type controls (Wilcoxon two‐sample test; n = 8).BWestern blot analysis of KDM6A expression in glomeruli and podocytes isolated from wild‐type and KDM6A‐KO mice. Presented experiments were performed at least three times independently.CChanges in body weights and levels of blood glucose in wild‐type or KDM6A‐KO mice with or without STZ treatment. No significant differences in body weights or blood glucose levels between wild‐type and KDM6A‐KO mice with diabetes were observed during the 8‐week experimental period (Wilcoxon two‐sample test; n = 8).DLevels of urinary protein excretion, weights of kidney, and levels of HbA1c in wild‐type and KDM6A‐KO mice with or without STZ treatment (at 8 weeks after the onset of diabetes). The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P < 0.05 versus untreated wild‐type controls, #P < 0.05 versus STZ‐treated wild‐type mice (parametric ANOVA and a Bonferroni post hoc test; n = 8).EImmunofluorescence images of kidney sections stained with KDM6A and nephrin in wild‐type (WT‐NC), KDM6A‐KO (KO‐NC), STZ‐treated wild‐type (WT‐DM), or STZ‐treated KDM6A‐KO (KO‐DM) mice. Scale bars, 20 μm. *P < 0.05 versus untreated wild‐type controls, #P < 0.05 versus STZ‐treated wild‐type mice (parametric ANOVA and a Bonferroni post hoc test; n = 3).FImmunofluorescence images of kidney sections stained with KDM6A and H3K27me3 in wild‐type (WT‐NC), KDM6A‐KO (KO‐NC), STZ‐treated wild‐type (WT‐DM), or STZ‐treated KDM6A‐KO (KO‐DM) mice. Scale bars, 20 μm. *P < 0.05 versus untreated wild‐type controls, #P < 0.05 versus STZ‐treated

<b>Biological Strategies Validation. </b>KLF10 is a downstream effector of KDM6A and is capable of directly binding to nephrin gene promoterAScreening of the potential KDM6A‐regulated transcriptional factors involved in repression of nephrin expression. After transduction with a control lentiviral vector or a lentiviral vector expressing KDM6A into primary podocytes for 48 h, intracellular RNAs were isolated and used for RNA sequencing (RNA‐Seq) analysis. The transcript expression patterns of 86 selected transcriptional factors from three independent RNA‐Seq experiments are presented in a heat map. Notably, among these 86 selected genes, KLF10 is the most up‐regulated gene.BValidation of increased KDM6A and KLF10 expression in primary podocytes that were infected with an empty lentiviral vector or KDM6A‐expressing lentiviral vector for 48 h. *P < 0.05 versus the empty vector control (Wilcoxon two‐sample test; n = 3).CIncreased expression of KDM6A and KLF10 in primary podocytes cultured in high glucose for 48 h. *P < 0.05 versus normal controls (Wilcoxon two‐sample test; n = 3).DEffect of KLF10 knockdown on high glucose‐mediated reduction of nephrin in primary podocytes. As noted, knockdown of KLF10 prevented down‐regulation of nephrin and up‐regulation of KDM6A in high glucose‐treated podocytes. *P < 0.05 versus normal controls, #P < 0.05 versus control siRNA with HG incubation (parametric ANOVA and a Bonferroni post hoc test; n = 3).EImmunofluorescence analysis of KLF10 and nephrin in renal sections of normal, diabetic, and GSK‐J4‐treated diabetic mice. Scale bars, 20 μm. *P < 0.05 versus the normal control group, #P < 0.05 versus the untreated diabetic group (parametric ANOVA and a Bonferroni post hoc test; n = 3).FImmunofluorescence images of KLF10 and nephrin in renal sections of wild‐type or KDM6A‐KO mice with or without STZ treatment. Scale bars, 20 μm. *P < 0.05 versus the untreated wild‐type group, #P < 0.05 versus the STZ‐treated wild‐type group (parametric ANOVA and a Bonferroni post hoc test; n

Elevated levels of KDM6A and KLF10 expression, along with decreased levels of nephrin (or WT‐1) expression, are detected in kidney tissues and urinary exosomes of patients with diabetic nephropathyAImmunofluorescence analysis of kidney sections from diabetic nephropathy subjects and non‐diabetic controls stained with KDM6A, KLF10, nephrin, and WT‐1. Scale bars, 50 μm. *P < 0.05 by Wilcoxon two‐sample test (n = 6 for each group).BWestern blot analysis of kidney tissues from non‐diabetic controls and diabetic nephropathy subjects. Expression levels of KDM6A, KLF10, nephrin, and WT‐1 in kidney tissues were determined by immunoblotting using the indicated antibodies. Relative protein levels in kidney tissues were normalized to actin. *P < 0.05 by Wilcoxon two‐sample test (n = 6 for each group).CLevels of KDM6A and KLF10, nephrin mRNAs in urine exosomes of diabetic nephropathy patients and non‐diabetic controls. Relative mRNA levels in human urinary exosomes were normalized to 18S rRNA. Horizontal lines are medians. *P < 0.05, **P < 0.01, and ***P < 0.001 by Wilcoxon two‐sample test (n = 12 for each group).Data information: Data are expressed as mean ± SEM (A and B). See the exact P‐values for comparison tests in Appendix Table S7. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30948420), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>KDM6A promotes podocyte and kidney dysfunction in diabetic miceARelative mRNA levels of KDM6A, nephrin and WT‐1, normalized to beta ‐actin, expressed in kidney glomeruli of normal and diabetic mice at 4, 8, and 12 weeks after diabetic induction. *Significant differences (P < 0.05) compared with normal controls (Wilcoxon two‐sample test; n = 8 each).BWestern blot analysis of nephrin, KDM6A, and H3K27me3 in kidney glomeruli of normal and diabetic mice at different time points. *P < 0.05 versus normal controls for the indicated time points (Wilcoxon two‐sample test; n = 3).CChanges in body weights and levels of blood glucose in normal, diabetic, and GSK‐J4‐treated diabetic mice. As noted, GSK‐J4 treatment did not significantly affect body weights or blood glucose levels in diabetic mice during the experimental period of 12 weeks (Wilcoxon two‐sample test; n = 8).DLevels of urinary protein excretion, weights of kidney, and levels of HbA1c in normal, diabetic, and GSK‐J4‐treated diabetic mice. Urinary total protein excretion, kidney weight, and levels of HbA1c were measured at 12 weeks after diabetic induction. The mean relative kidney weight (%) shown in the study is determined as the percent of kidneys out of total body weight, and the HbA1c level is defined as the ratio of HbA1c to the total hemoglobin (% HbA1c; DCCT unit). *P < 0.05 versus normal controls, #P < 0.05 versus untreated diabetic mice (parametric ANOVA and a Bonferroni post hoc test; n = 8).EWestern blot analysis of KDM6A, nephrin, WT‐1, and H3K27me3 expressed in kidney glomeruli of normal, diabetic, and GSK‐J4‐treated diabetic mice at 12 weeks after diabetic induction. *P < 0.05 versus normal controls, #P < 0.05 versus untreated diabetic mice (parametric ANOVA and a Bonferroni post hoc test; n = 3).FImmunofluorescence analysis of KDM6A, nephrin, and WT‐1 in kidney sections from normal, diabetic, and GSK‐J4‐treated diabetic mice. Green: nephrin or WT‐1; red: KDM6A; blue: DAPI. Scale bars, 20 μm. *P < 0.05 ver

Comparison of nephrin imaging with different conventional and super-resolution modalities. a Widefield vs dSTORM using Atto647N. b Confocal vs STED using Atto647N. c Widefield vs SIM using Alexa Fluor 488. d ExM prepared sample imaged with widefield vs e confocal using Alexa Fluor 488. The confocal ExM image furthermore shows the nucleus stained with Hoechst. Highlighted panels show × 3 zoomed areas. Scale bars in a–c are 1 μm, in d and e 10 μm and in the zoomed in areas 5 μm (after expansion) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33277998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Kidney organoids derived from the isolated Sca1+ Oct4+ cells develop kidney structures and glomerular lineage. A) The time‐course of the development of Sca1+ Oct4+ cell‐derived kidney organoids in culture. Scale bar, 100 µm. B) Bright field of kidney‐shaped KSCs‐derived organoid. Scale bar, 200 µm. C) Cryosection of kidney organoids (H&E staining) revealing kidney structures including cortex, medulla, column, and capsule. Scale bar, 20 µm. D) Cryosections of kidney organoids were further processed with immunofluorescent staining. The photo shows glomerulus‐like structure with positive staining for nephrin (red) and distal tubule‐positive staining for NCC (green, arrow). Scale bar, 10 µm. Data is representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35315252), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation of a self‐organizing de novo nephron from the differentiated Sca1+ Oct4+ monolayer cultures. A) Bright field image of self‐organizing organoid‐like structure from monolayer culture of kidney stem cells. The photo shows glomerulus‐like structure (black arrow), tubule‐like structure (red arrows), and collecting duct‐like structure (green arrow). Scale bar, 100 µm. A1) Glomerulus‐like structure stained with nephrin (white arrow). Scale bar, 50 µm. A2) Glomerulus‐like structure stained with nephrin (white arrow) and proximal tubule‐like structure stained with LTL (red arrows). Scale bar, 50 µm. A3) Glomerulus‐like structure stained with podocin (white arrow). Scale bar, 50 µm. A4) Collecting duct‐like structure stained with GATA3 (white arrow). Scale bar, 50 µm. ,C) Western blot analysis of CD31, nephrin, and podocin expression in undifferentiated and differentiated Sca1+ Oct4+ cells. GAPDH was used as a loading control. Data is representative of three independent experiments. Values represent the mean ± SD (n = 3). p < 0.05 versus control by 2‐tailed Student's t test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35315252), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation of self‐organizing kidney‐shaped structures from the differentiated Sca1+ Oct4+ monolayer cultures. A) Comma‐shaped body with positive staining of cleaved‐Notch1 and CD31. Scale bar, 20 µm. B) Bright field (BF) of differentiation of the isolated Sca1+ Oct4+ cells and subsequent generation of self‐organized kidney‐shaped structures. Scale bar, 100, 20, and 20 µm. C) Expression of nephrin and podocin in self‐organizing kidney‐shaped structures assembled by differentiated Sca1+ Oct4+ cells. Scale bar, 25 µm. D) Uptake of 70 kDa FITC‐dextran by kidney‐shaped structures after 30 min and 6 h. Scale bar, 25 µm. Data is representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35315252), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3159U-100UG
Alexa Fluor 405	AF3159V-100UG
Alexa Fluor 488	AF3159G-100UG
Alexa Fluor 532	AF3159X-100UG
Alexa Fluor 594	AF3159T-100UG
Alexa Fluor 647	AF3159R-100UG
Alexa Fluor 700	AF3159N-100UG
Alexa Fluor 750	AF3159S-100UG
Alexa Fluor Plus 405	AF3159AFP405-100UG
Alexa Fluor Plus 488	AF3159AFP488-100UG
Alexa Fluor Plus 555	AF3159AFP555-100UG
Alexa Fluor Plus 594	AF3159AFP594-100UG
Alexa Fluor Plus 647	AF3159AFP647-100UG
Alexa Fluor Plus 680	AF3159AFP680-100UG
Allophycocyanin	AF3159A
Biotin	BAF3159
CoraFluor 1	AF3159CL1
DyLight 350	AF3159D
DyLight 405	AF3159E
DyLight 488	AF3159K
DyLight 550	AF3159L
DyLight 594	AF3159M
DyLight 650	AF3159W
DyLight 680	AF3159Y
DyLight 755	AF3159Z
FITC	AF3159F
HRP	AF3159H
Janelia Fluor 525	AF3159JF525
Janelia Fluor 549	AF3159I
Janelia Fluor 646	AF3159J
Janelia Fluor 669	AF3159JF669
PE	AF3159P
PerCP	AF3159C
mFluor Violet 450 SE	AF3159MFV450
mFluor Violet 500 SE	AF3159MFV500
mFluor Violet 610 SE	AF3159MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3159-SP

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AF4269

Nephrin Antibody [Unconjugated]

(56 Publications)

100 μg

AF4269

HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Nephrin was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Nephrin at approximately 185 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Concordant expression of developmental programs across organoids from four human iPSC lines. a Heatmap of expression patterns for major nephrogenesis markers across organoid differentiation time points (iPSC D0, D7, D15, and D29, averaged across four cell lines, ML protocol) and human adult kidney. Expression values were row-normalized to obtain z-scores; red color indicates positive z-scores. b Canonical (NPHS2) and data-derived (CLDN5) podocyte marker genes superimposed in tSNE plots from D15 organoids (N2 line, ML protocol). c IF staining of D15 kidney organoid (N2 line, ML protocol) for CLDN5 as a marker of early podocyte differentiation derived from the single-cell data. Additional canonical podocyte markers (NPHS1, WT1) and DAPI staining as shown. d IF staining of D29 kidney organoid (AS line, ML protocol) for SOX17 and CD31, markers of endothelial cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31784515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Organoid Glomeruli model of congenital nephrotic syndrome in vitro. a Description of the NPHS1 variants identified in the patient modelled, diagnosed with congenital nephrotic syndrome (CNS). b–d Immunostaining of OrgGloms isolated from control organoids and CNS patient organoids show reduced NEPHRIN and PODOCIN protein levels in the organoids derived from patient-iPSC, representative images shown of >3 biological replicates. Scale bars 10 µm. e Higher power immunofluorescent images show the polarised co-localisation of NEPHRIN with NEPH1 (solid white arrowheads) and PODOCIN in control OrgGloms. This polarisation is lost in CNS OrgGloms due to the absence of NEPHRIN (white arrows). Scale bars 10 µm. f Quantitative analysis of fluorescence intensities from independent OrgGlom biological replicates performed using one control and two distinct patient-derived CNS iPSC clones. Organoid glomeruli generated from both patient-derived iPSC clones show significant reduction in NEPHRIN and PODOCIN protein levels. Two-way ANOVA p < 0.0001; error bars = SEM. Biological replicates. NEPHRIN (controls, n = 20; CNS, n = 56); PODOCIN (controls, n = 14; CNS, n = 22); CD2AP (controls, n = 8; CNS, n = 15); NEPH1 (controls, n = 10; CNS, n = 17). Significant difference assessed by Sidak’s multiple comparisons test between cell lines; F-value = 112; DF = 1. NEPHRIN: control vs CNS#1, p < 0.0001; control vs CNS#2, p < 0.0001; CNS#1 vs CNS#2, p > 0.9999. PODOCIN: control vs CNS#1, p < 0.0001; control vs CNS#2, p < 0.0001; CNS#1 vs CNS#2, p = 0.9995. CD2AP: control vs CNS#1, p = 0.0007; control vs CNS#2, p = 0.0016; CNS#1 vs CNS#2, p = 0.9980. NEPH1: control vs CNS#1, p = 0.5320; control vs CNS#2, p = 0.9994; CNS#1 vs CNS#2, p = 0.9992. g Quantitative western blot analysis of NEPHRIN and PODOCIN protein levels within independent biological replicates confirms the significant depletion of these proteins in OrgGloms derived from CNS iPSCs Image collected and cropped by CiteAb from the followin

IF validation of markers derived from the single-cell data in mature kidney organoids. a IF staining of an entire kidney organoid with segment specific markers as shown. b Schematic of kidney nephron with major cell types and canonical markers annotated. c Immunofluorescence staining of D29 kidney organoids for podocyte (WT1), proximal tubule (LTL), and distal tubule (CDH1 and GATA3) across two protocols (JB, ML) and four cell lines (AS, N1, N2, ThF). IF staining for validation of markers identified in the single-cell data: d NPHS1 colocalized with the podocyte-specific marker SYNPO and e LRP2 colocalized with the proximal tubular marker LTL (bottom). f IF staining validation for MEIS1-positive mesenchymal cells in D29 organoids. LAMA1 indicates basement membranes. MEIS1 staining of mesenchymal cells appropriately surrounds LAMA1-defined tubular nephron structures. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31784515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4269U-100UG
Alexa Fluor 405	AF4269V-100UG
Alexa Fluor 488	AF4269G-100UG
Alexa Fluor 532	AF4269X-100UG
Alexa Fluor 594	AF4269T-100UG
Alexa Fluor 647	AF4269R-100UG
Alexa Fluor 700	AF4269N-100UG
Alexa Fluor 750	AF4269S-100UG
Alexa Fluor Plus 405	AF4269AFP405-100UG
Alexa Fluor Plus 488	AF4269AFP488-100UG
Alexa Fluor Plus 555	AF4269AFP555-100UG
Alexa Fluor Plus 594	AF4269AFP594-100UG
Alexa Fluor Plus 647	AF4269AFP647-100UG
Alexa Fluor Plus 680	AF4269AFP680-100UG
Allophycocyanin	AF4269A
Biotin	BAF4269
CoraFluor 1	AF4269CL1
DyLight 350	AF4269D
DyLight 405	AF4269E
DyLight 488	AF4269K
DyLight 550	AF4269L
DyLight 594	AF4269M
DyLight 650	AF4269W
DyLight 680	AF4269Y
DyLight 755	AF4269Z
FITC	AF4269F
HRP	AF4269H
Janelia Fluor 525	AF4269JF525
Janelia Fluor 549	AF4269I
Janelia Fluor 646	AF4269J
Janelia Fluor 669	AF4269JF669
PE	AF4269P
PerCP	AF4269C
mFluor Violet 450 SE	AF4269MFV450
mFluor Violet 500 SE	AF4269MFV500
mFluor Violet 610 SE	AF4269MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4269-SP

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NBP1-77303

Nephrin Antibody - BSA Free

(8 Publications)

0.1 mg

NBP1-77303

<b>Biological Strategies Validation. </b>Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - In vitro AS-IV effects on SIRT1 and NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT.Podocytes were pretreated with high glucose for 1hour, and then incubated with PDTC or AS-IV for 48hours. The levels of TGF-, nephrin, E-cadherin, N-cadherin and -SMA were quantified with Western blotting. Data is presented as meanSD. n=3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P <0.05. Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT-NF-kB p65 axis. Sci Rep (2019)

Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.

Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - In vivo AS-IV effects on podocyte EMT. alpha -SMA & nephrin levels in vivo were analyzed by immunofluorescence assay & the levels of TGF-beta , N-cadherin & E-cadherin were quantified using Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal control cohort, or AS-IV treated cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - AS-IV effects on hyperglycaemia-triggered podocyte EMT. (A) AS-IV chemical structure. (B–E) Podocytes were pretreated with high/normal glucose for 1 hour, & then incubated with or without AS-IV (25, 50 & 100 μM) for 48 hours. (B) mRNA expression levels of TGF-beta were detected using real-time PCR. (C) TGF-beta protein levels were quantified using Western blotting. (D) mRNA expression levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using real-time PCR. (E) Protein levels of alpha -SMA, N-cadherin, E-cadherin & nephrin were detected using Western blotting. Note: E-Ca, E-cadherin; N-Ca, N-cadherin. The molecular weight of the proteins: TGF-beta , 44 kDa; nephrin, 100 kDa; E-cadherin, 110 kDa; N-cadherin, 100 kDa; alpha -SMA, 42 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort or AS-IV cohort, P < 0.05; #compared with high glucose cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Nephrin Antibody - BSA Free [NBP1-77303] - In vitro AS-IV effects on SIRT1 & NF-kB p65 expression in hyperglycaemia-triggered podocyte EMT. (A–F) Podocytes were exposed to either a SIRT1 inhibitor EX527 or SIRT1 activator SRT1720 after a 1 hour exposure to hyperglycaemic conditions, & were subsequently incubated with or without AS-IV (100 μM) for 48 hours. (A,E) mRNA expression levels of SIRT1and p65 were quantified using real-time PCR. (B) The deacetylase activity of SIRT1 was detected with a SIRT1 activity assay. (C,D,F) The protein levels of SIRT1 & AC-p65 were quantified using Western blotting. The molecular weight of the proteins: SIRT1, 110 kDa; AC-p65, 65 kDa; p65, 65 kDa. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or AS-IV cohort, or high glucose plus AS-IV cohort, or high glucose plus SRT1720 cohort, P < 0.05; #compared with high glucose plus AS-IV cohort, P < 0.05. (G) Podocytes were pretreated with high glucose for 1 hour, & then incubated with PDTC or AS-IV for 48 hours. The levels of TGF-beta , nephrin, E-cadherin, N-cadherin & alpha -SMA were quantified with Western blotting. Data is presented as mean ± SD. n = 3. *Compared with normal glucose cohort, or high glucose plus AS-IV cohort, or high glucose plus PDTC cohort, P < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30674969), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- Nephrin antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Mouse Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney cells labeling Nephrin with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry/ Immunofluorescence: Nephrin Antibody - BSA Free [NBP1-77303] - Validation of Nephrin in Rat Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Nephrin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77303AF350
Alexa Fluor 405	NBP1-77303AF405
Alexa Fluor 488	NBP1-77303AF488
Alexa Fluor 532	NBP1-77303AF532
Alexa Fluor 594	NBP1-77303AF594
Alexa Fluor 647	NBP1-77303AF647
Alexa Fluor 700	NBP1-77303AF700
Alexa Fluor 750	NBP1-77303AF750
Alexa Fluor Plus 488	NBP1-77303AFP488
Alexa Fluor Plus 555	NBP1-77303AFP555
Alexa Fluor Plus 594	NBP1-77303AFP594
Alexa Fluor Plus 647	NBP1-77303AFP647
Alexa Fluor Plus 680	NBP1-77303AFP680
Allophycocyanin	NBP1-77303APC
Biotin	NBP1-77303B
CoraFluor 1	NBP1-77303CL1
DyLight 350	NBP1-77303UV
DyLight 405	NBP1-77303V
DyLight 488	NBP1-77303G
DyLight 550	NBP1-77303R
DyLight 594	NBP1-77303DL594
DyLight 650	NBP1-77303C
DyLight 680	NBP1-77303FR
DyLight 755	NBP1-77303IR
FITC	NBP1-77303F
HRP	NBP1-77303H
Janelia Fluor 525	NBP1-77303JF525
Janelia Fluor 549	NBP1-77303JF549
Janelia Fluor 646	NBP1-77303JF646
Janelia Fluor 669	NBP1-77303JF669
PE	NBP1-77303PE
PerCP	NBP1-77303PCP
mFluor Violet 450 SE	NBP1-77303MFV450
mFluor Violet 500 SE	NBP1-77303MFV500
mFluor Violet 610 SE	NBP1-77303MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77303-0.025mg

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NBP1-30130

Nephrin Antibody (Y17-R)

(1 Review)

(10 Publications)

0.05 ml

NBP1-30130

Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins.(A) Representative images of Western blots for five proteins critical for physiological function of podocytes. Images of the complete blots are shown in Figure S3. (B) Quantification of kidney podocyte protein markers. Highest upregulation of WT-1 was found on the 2 kPa gel (2.2 ± 0.3). Nephrin, podocin, & CD2AP showed similar trends. Synaptopodin did not show this trend & there was no statistical difference between softer gels & control. T-statistics showed statistically significant changes of the ratios of five protein markers after normalization by control (two-tails, alpha = 0.05). The average t-statistics were 4.5 for the 2 kPa gel & 4.0 for the 5 kPa gel, which were significantly different relative to the control. Those for the 0.6 kPa and 13 kPa gels were 0.8 & 1.5, & the differences were not significant. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep43934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-18058

Nephrin Antibody - BSA Free

0.1 mg

NBP3-18058

Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-18058AF350
Alexa Fluor 405	NBP3-18058AF405
Alexa Fluor 488	NBP3-18058AF488
Alexa Fluor 532	NBP3-18058AF532
Alexa Fluor 594	NBP3-18058AF594
Alexa Fluor 647	NBP3-18058AF647
Alexa Fluor 700	NBP3-18058AF700
Alexa Fluor 750	NBP3-18058AF750
Alexa Fluor Plus 405	NBP3-18058AFP405
Alexa Fluor Plus 488	NBP3-18058AFP488
Alexa Fluor Plus 555	NBP3-18058AFP555
Alexa Fluor Plus 594	NBP3-18058AFP594
Alexa Fluor Plus 647	NBP3-18058AFP647
Alexa Fluor Plus 680	NBP3-18058AFP680
Allophycocyanin	NBP3-18058APC
Biotin	NBP3-18058B
CoraFluor 1	NBP3-18058CL1
DyLight 350	NBP3-18058UV
DyLight 405	NBP3-18058V
DyLight 488	NBP3-18058G
DyLight 550	NBP3-18058R
DyLight 594	NBP3-18058DL594
DyLight 650	NBP3-18058C
DyLight 680	NBP3-18058FR
DyLight 755	NBP3-18058IR
FITC	NBP3-18058F
HRP	NBP3-18058H
Janelia Fluor 525	NBP3-18058JF525
Janelia Fluor 549	NBP3-18058JF549
Janelia Fluor 646	NBP3-18058JF646
Janelia Fluor 669	NBP3-18058JF669
PE	NBP3-18058PE
PerCP	NBP3-18058PCP
mFluor Violet 450 SE	NBP3-18058MFV450
mFluor Violet 500 SE	NBP3-18058MFV500
mFluor Violet 610 SE	NBP3-18058MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-18058SS

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NBP1-30137

Nephrin Antibody (G17-H)

0.05 ml

NBP1-30137

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-76750

Mouse Nephrin ELISA Kit (Colorimetric)

1 Kit

NBP2-76750

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.156 - 10ng/mL

Sensitivity:

0.094ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-76751

Rat Nephrin ELISA Kit (Colorimetric)

(3 Publications)

1 Kit

NBP2-76751

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Standard Curve:

0.156 - 10ng/mL

Sensitivity:

0.094ng/mL

Sample Type:

Serum, plasma and other biological fluids

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3159-NN

Recombinant Mouse Nephrin Protein, CF

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP2-37834

Nephrin Antibody - BSA Free

0.1 mg

NBP2-37834

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-37834AF350
Alexa Fluor 405	NBP2-37834AF405
Alexa Fluor 488	NBP2-37834AF488
Alexa Fluor 532	NBP2-37834AF532
Alexa Fluor 594	NBP2-37834AF594
Alexa Fluor 647	NBP2-37834AF647
Alexa Fluor 700	NBP2-37834AF700
Alexa Fluor 750	NBP2-37834AF750
Alexa Fluor Plus 405	NBP2-37834AFP405
Alexa Fluor Plus 488	NBP2-37834AFP488
Alexa Fluor Plus 555	NBP2-37834AFP555
Alexa Fluor Plus 594	NBP2-37834AFP594
Alexa Fluor Plus 647	NBP2-37834AFP647
Alexa Fluor Plus 680	NBP2-37834AFP680
Allophycocyanin	NBP2-37834APC
Biotin	NBP2-37834B
CoraFluor 1	NBP2-37834CL1
DyLight 350	NBP2-37834UV
DyLight 405	NBP2-37834V
DyLight 488	NBP2-37834G
DyLight 550	NBP2-37834R
DyLight 594	NBP2-37834DL594
DyLight 650	NBP2-37834C
DyLight 680	NBP2-37834FR
DyLight 755	NBP2-37834IR
FITC	NBP2-37834F
HRP	NBP2-37834H
Janelia Fluor 525	NBP2-37834JF525
Janelia Fluor 549	NBP2-37834JF549
Janelia Fluor 646	NBP2-37834JF646
Janelia Fluor 669	NBP2-37834JF669
PE	NBP2-37834PE
PerCP	NBP2-37834PCP
mFluor Violet 450 SE	NBP2-37834MFV450
mFluor Violet 500 SE	NBP2-37834MFV500
mFluor Violet 610 SE	NBP2-37834MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37834-0.025mg

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NBP1-77303PEP

Nephrin Antibody Blocking Peptide

0.05 mg

NBP1-77303PEP

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-10533

Nephrin Overexpression Lysate

0.1 mg

NBP2-10533

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

NPHS1 Products By Gene Name

Nephrin Antibody [Unconjugated]

Nephrin Antibody [Unconjugated]

Nephrin Antibody - BSA Free

Nephrin Antibody (Y17-R)

Nephrin Antibody - BSA Free

Nephrin Antibody (G17-H)

Mouse Nephrin ELISA Kit (Colorimetric)

Rat Nephrin ELISA Kit (Colorimetric)

Recombinant Mouse Nephrin Protein, CF

Nephrin Antibody - BSA Free

Nephrin Antibody Blocking Peptide

Nephrin Overexpression Lysate