Podocin/NPHS2 Antibody (JB51-33)

Images

 
Western Blot: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen ...read more
Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded human kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JB51-33
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Podocin/NPHS2 Antibody (JB51-33) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human Podocin/NPHS2 aa 334-383 / 383. (SwissProt: Q9NP85 Human; SwissProt: Q91X05 Mouse; SwissProt: Q8K4G9 Rat)
Localization
Cell membrane. Endoplasmic reticulum.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NPHS2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-75624 in the following applications:

  • 1 publication
  • 3 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Podocin/NPHS2 Antibody (JB51-33)

  • Nephrosis 2
  • nephrosis 2, idiopathic, steroid-resistant (podocin)
  • NPHS2
  • PDCN
  • PDCNSRN1podocin
  • Podocin
  • SRN1

Background

NPHS2 encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3159
Species: Mu
Applications: IHC
H00000081-M01
Species: Hu, Pm, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-90625
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-39100
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB110-60011
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP1-85047
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
AF1638
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
H00003845-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IP, WB
NBP2-20383
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-02623
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-77260
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF1556
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
NBP2-41194
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
DVE00
Species: Hu
Applications: ELISA
AF3844
Species: Hu, Mu
Applications: IHC
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

Publications for Podocin/NPHS2 Antibody (NBP2-75624)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IBEX, IHC-P, WB.


Filter By Application
IBEX
(1)
IHC-P
(3)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(1)
All Species

Reviews for Podocin/NPHS2 Antibody (NBP2-75624) (0)

There are no reviews for Podocin/NPHS2 Antibody (NBP2-75624). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Podocin/NPHS2 Antibody (NBP2-75624) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Podocin/NPHS2 Products

Bioinformatics Tool for Podocin/NPHS2 Antibody (NBP2-75624)

Discover related pathways, diseases and genes to Podocin/NPHS2 Antibody (NBP2-75624). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Podocin/NPHS2 Antibody (NBP2-75624)

Discover more about diseases related to Podocin/NPHS2 Antibody (NBP2-75624).
 

Pathways for Podocin/NPHS2 Antibody (NBP2-75624)

View related products by pathway.

PTMs for Podocin/NPHS2 Antibody (NBP2-75624)

Learn more about PTMs related to Podocin/NPHS2 Antibody (NBP2-75624).
 

Research Areas for Podocin/NPHS2 Antibody (NBP2-75624)

Find related products by research area.

Blogs on Podocin/NPHS2

There are no specific blogs for Podocin/NPHS2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Podocin/NPHS2 Antibody (JB51-33) and receive a gift card or discount.

Bioinformatics

Gene Symbol NPHS2