CD2AP Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Analysis in human small intestine and skeletal muscle tissues. Corresponding CD2AP RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney, placenta, skeletal muscle and small intestine using Anti-CD2AP antibody NBP1-90625 (A) shows similar ...read more
Orthogonal Strategies: Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in human cell lines A-431 and PC-3 using anti-CD2AP antibody. Corresponding CD2AP RNA-seq data are presented for the same cell lines. ...read more
Western Blot: CD2AP Antibody [NBP1-90625] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: CD2AP Antibody [NBP1-90625] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human small intestine shows strong granular positivity in cytoplasm in glandular cells.
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: CD2AP Antibody [NBP1-90625] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD2AP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VDFDSLTVRTSKETDVVNFDDIASSENLLHLTANRPKMPGRRLPGRFNGGHSPTHSPEKILKLPKEEDSANLKPSELKKDTCYSPKPSVYLSTPSSASKANTTAFLTPLEIKAKVETDDVKKNSLDELRAQIIELLCIVEALKKDHGKEL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD2AP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CD2AP Protein (NBP1-90625PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CD2AP Antibody

  • adapter protein CMS
  • Cas ligand with multiple Src homology 3 (SH3) domains
  • CD2AP
  • CD2-associated protein
  • CMS
  • DKFZp586H0519

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD2AP Antibody (NBP1-90625) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CD2AP Products

Array NBP1-90625

Research Areas for CD2AP Antibody (NBP1-90625)

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Blogs on CD2AP

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol CD2AP