Nephrin Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Nephrin Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Gln37-Thr1049
Accession # Q9QZS7
Specificity
Detects mouse Nephrin in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Nephrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPHS1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Biotin]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

  1. Kawachi, H. et al. (2006) Nephrology 11:274.
  2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
  3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
  4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
  5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
  6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
  7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
  8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
  9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
  10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
  11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
  12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
  13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
  14. Coward, R.J. et al. (2007) Diabetes 56:1127.
  15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Biotin BAF3159

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Bioinformatics

Gene Symbol NPHS1
Uniprot
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