Nephrin Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Nephrin Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
  Gln37-Thr1049
  Accession # Q9QZS7
• Specificity: Detects mouse Nephrin in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Nephrin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NPHS1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Biotin]

• CNF
• Nephrin
• nephrosis 1, congenital, Finnish type (nephrin)
• NPHNCNF
• NPHS1
• Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

1. Kawachi, H. et al. (2006) Nephrology 11:274.
2. Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
3. Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
4. Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
5. Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
6. Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
7. Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
8. Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
9. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
10. Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
11. Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
12. Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
13. Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
14. Coward, R.J. et al. (2007) Diabetes 56:1127.
15. Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nephrin Antibody (BAF3159)(3)

Publications using BAF3159

• Kim, HS;Lee, HK;Kim, K;Ahn, GB;Kim, MS;Lee, TY;Son, DJ;Kim, Y;Hong, JT;Han, SB; Mesenchymal stem cells enhance CCL8 expression by podocytes in lupus-prone MRL.Faslpr mice Scientific reports 2023-08-11 [PMID: 37567910] (MACS, Transgenic Mouse)
• Wan Q, Zhou J, Wu Y et al. TNF-?-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy Renal Failure 12/31/2022 [PMID: 35837694]
• Wan Q, Zhou J, Wu Y et al. TNF-?-mediated podocyte injury via the apoptotic death receptor pathway in a mouse model of IgA nephropathy Renal Failure 2022-12-31 [PMID: 35837694]

Bioinformatics

• Gene Symbol: NPHS1
• Uniprot:
  • Q9QZS7()