Nephrin Antibody [Unconjugated]

Images

 
Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Nephrin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nephrin
Gln23-Thr1029
Accession # O60500
Specificity
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NPHS1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
Control
Nephrin Knockout HeLa Cell Lysate
Publications
Read Publications using
AF4269 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Unconjugated]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (AF4269)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, IHC-P.


Filter By Application
IHC
(2)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF4269 Applications Species
JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler,, Organoid single cell profiling identifies a transcriptional signature of glomerular disease JCI Insight, 2019;4(1):. 2019 [PMID: 30626756] (IHC-P, Human) IHC-P Human
B Phipson, PX Er, AN Combes, TA Forbes, SE Howden, L Zappia, HJ Yen, KT Lawlor, LJ Hale, J Sun, E Wolvetang, M Takasato, A Oshlack, MH Little Evaluation of variability in human kidney organoids Nat. Methods, 2019;16(1):79-87. 2019 [PMID: 30573816] (IHC, Mouse) IHC Mouse
LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening Nat Commun, 2018;9(1):5167. 2018 [PMID: 30514835] (Human) Human
H Wu, K Uchimura, EL Donnelly, Y Kirita, SA Morris, BD Humphreys Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30449713] (IHC-Fr, Human) IHC-Fr Human
ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions Sci Rep, 2016;6(0):37172. 2016 [PMID: 27849017] (IHC-P, Human) IHC-P Human
Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature, 2015;526(7574):564-8. 2015 [PMID: 26444236] (IHC, Human) IHC Human

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF4269

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Bioinformatics

Gene Symbol NPHS1
Uniprot