Nephrin Antibody [Unconjugated]


Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at more
Simple Western lane view shows lysates of human kidney, loaded at 0.2 mg/mL. A specific band was detected for Nephrin at approximately 185 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Nephrin Antigen more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC

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Nephrin Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Nephrin
Accession # O60500
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF4269 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Unconjugated]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor


Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (AF4269)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.

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Flow Cytometry
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF4269 Applications Species
G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation Cell Stem Cell, 2022;29(11):1580-1593.e7. 2022 [PMID: 36332571] (IHC, Human) IHC Human
AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel Transplanted organoids empower human preclinical assessment of drug candidate for the clinic Science Advances, 2022;8(27):eabj5633. 2022 [PMID: 35857479] (Flow Cytometry, Human) Flow Cytometry Human
N Won, J Castillo-P, X Tan, J Ford, D Heath, LI Mazilescu, M Selzner, IM Rogers Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days Cells, 2022;11(11):. 2022 [PMID: 35681517] (IHC, Mouse) IHC Mouse
J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids Cell Stem Cell, 2021;29(2):217-231.e8. 2021 [PMID: 35032430] (IHC, Human) IHC Human
L Miesen, P Bándi, B Willemsen, F Mooren, T Strieder, E Boldrini, V Drenic, J Eymael, R Wetzels, J Lotz, N Weiss, E Steenberge, TH van Kuppev, M van Erp, J van der La, N Endlich, MJ Moeller, JFM Wetzels, J Jansen, B Smeets Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis Disease Models & Mechanisms, 2021;0(0):. 2021 [PMID: 34927672] (IHC, Rat) IHC Rat
R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34847364] (IHC, Human) IHC Human
KS Lee, J Lee, P Lee, BC Jeon, MY Song, S Kwak, J Lee, JS Kim, DJ Kim, JH Kim, VL Tesh, MS Lee, SK Park Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host Embo Molecular Medicine, 2021;0(0):e14678. 2021 [PMID: 34842355] (IHC, Human) IHC Human
S Ueda, T Tominaga, A Ochi, A Sakurai, K Nishimura, E Shibata, S Wakino, M Tamaki, K Nagai TGF-beta1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction Scientific Reports, 2021;11(1):21643. 2021 [PMID: 34737348] (IHC, Human) IHC Human
JW Kim, HW Kim, SA Nam, JY Lee, HJ Cho, TM Kim, YK Kim Human kidney organoids reveal the role of glutathione in Fabry disease Experimental & Molecular Medicine, 2021;0(0):. 2021 [PMID: 34654880] (IHC, Human) IHC Human
N Mangold, J Pippin, D Unnersjoe-, S Koehler, S Shankland, S Brähler, B Schermer, T Benzing, PT Brinkkoett, H Hagmann The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development Cells, 2021;10(9):. 2021 [PMID: 34572114] (IHC, Mouse) IHC Mouse
Show All 21 Publications.

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Gene Symbol NPHS1