Nephrin Antibody [Unconjugated]


Western blot shows lysates of human kidney tissue under reducing and non-reducing conditions. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Nephrin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4269) overnight at more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC

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Nephrin Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Nephrin
Accession # O60500
Detects human Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Nephrin is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF4269 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephrin Antibody [Unconjugated]

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor


Nephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. It is expressed on podocytes and is an essential component of the interpodocyte-spanning slit diaphragm complex. Nephrin forms cis-hetero-oligomeric complexes with Neph1, followed by trans-homophilic interaction with Nephrin on opposing cells. Mutations in the Nephrin gene is the pathogenic cause of congenital nephrotic syndrome. Mature human Nephrin contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential soluble splice form is known where aa’s 1056-1095 are deleted, eliminating the transmembrane region. Over aa 23-1029, human Nephrin shares 84% and 89% aa sequence identity with mouse and canine Nephrin, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (AF4269)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF4269 Applications Species
L Miesen, P Bándi, B Willemsen, F Mooren, T Strieder, E Boldrini, V Drenic, J Eymael, R Wetzels, J Lotz, N Weiss, E Steenberge, TH van Kuppev, M van Erp, J van der La, N Endlich, MJ Moeller, JFM Wetzels, J Jansen, B Smeets Parietal epithelial cells maintain the epithelial cell continuum forming Bowman's space in focal segmental glomerulosclerosis Disease Models & Mechanisms, 2021;0(0):. 2021 [PMID: 34927672] (IHC, Rat) IHC Rat
R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34847364] (IHC, Human) IHC Human
KS Lee, J Lee, P Lee, BC Jeon, MY Song, S Kwak, J Lee, JS Kim, DJ Kim, JH Kim, VL Tesh, MS Lee, SK Park Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host Embo Molecular Medicine, 2021;0(0):e14678. 2021 [PMID: 34842355] (IHC, Human) IHC Human
S Ueda, T Tominaga, A Ochi, A Sakurai, K Nishimura, E Shibata, S Wakino, M Tamaki, K Nagai TGF-beta1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction Scientific Reports, 2021;11(1):21643. 2021 [PMID: 34737348] (IHC, Human) IHC Human
N Mangold, J Pippin, D Unnersjoe-, S Koehler, S Shankland, S Brähler, B Schermer, T Benzing, PT Brinkkoett, H Hagmann The Atypical Cyclin-Dependent Kinase 5 (Cdk5) Guards Podocytes from Apoptosis in Glomerular Disease While Being Dispensable for Podocyte Development Cells, 2021;10(9):. 2021 [PMID: 34572114] (IHC, Mouse) IHC Mouse
K Uchimura, H Wu, Y Yoshimura, BD Humphreys Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell Reports, 2020;33(11):108514. 2020 [PMID: 33326782] (IHC, Human) IHC Human
J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata Transcription factor 21 expression in injured podocytes of glomerular diseases Sci Rep, 2020;10(1):11516. 2020 [PMID: 32661376] (IHC, Human) IHC Human
Monteil V, Kwon H, Prado P, Hagelkruys A, Wimmer R, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero J, Wirnsberger G, Zhang H, Slutsky A, Conder R, Montserrat N, Mirazimi A, Penninger J Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2. Cell, 0;181(4):905-913.e7. 0 [PMID: 32333836] (IHC, Human) IHC Human
A Subramania, EH Sidhom, M Emani, K Vernon, N Sahakian, Y Zhou, M Kost-Alimo, M Slyper, J Waldman, D Dionne, LT Nguyen, A Weins, JL Marshall, O Rosenblatt, A Regev, A Greka Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation Nat Commun, 2019;10(1):5462. 2019 [PMID: 31784515] (ICC, Mouse) ICC Mouse
JL Harder, R Menon, EA Otto, J Zhou, S Eddy, NL Wys, C O'Connor, J Luo, V Nair, C Cebrian, JR Spence, M Bitzer, OG Troyanskay, JB Hodgin, RC Wiggins, BS Freedman, M Kretzler, , Organoid single cell profiling identifies a transcriptional signature of glomerular disease JCI Insight, 2019;4(1):. 2019 [PMID: 30626756] (IHC-P, Human) IHC-P Human
Show All 16 Publications.

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Gene Symbol NPHS1