Nephrin Antibody (Y17-R)

Images

 
Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130] - Nephrin expression in HEK293 cells, visualized with clonal rabbit antiNephrin monospecific antibody. Primary antibody dilution - 1:70

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
Y17-R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nephrin Antibody (Y17-R) Summary

Immunogen
Peptide derived from the centre of the fifth Ig-like domain of human nephrin. Antibody recognizes the epitope located between Cys465 - Cys528.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NPHS1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • ELISA 1:50000-1:100000
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation 1:10-1:500
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100. Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk.
Control
Nephrin Lysate (NBP2-65035)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-30130 in the following applications:

Publications
Read Publications using
NBP1-30130 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Nephrin Antibody (Y17-R)

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-75624
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
H00000081-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-39100
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-90625
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-60011
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85047
Species: Hu, Po
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KO
AF1556
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-20383
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02623
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-77260
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-30130
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IP

Publications for Nephrin Antibody (NBP1-30130)(3)

Review for Nephrin Antibody (NBP1-30130) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-30130:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Nephrin NBP1-30130
Enlarge
reviewed by:
Evren Azeloglu
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedImmortalized human podocytes
SpeciesHuman

Blocking

Blocking DetailsRockland casein blocking buffer, 2hrs, RT

Primary Anitbody

Dilution Ratio1:1000 in 5% milk in TBS-T, overnight, 4ºC

Secondary Antibody

Secondary DescriptionThermo goat anti-rabbit HRP
Secondary Concentration1:2000 in 5% milk in TBS-T, 30min, RT

Details

Detection NotesThermo ECL, film, 1min, RT

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nephrin Antibody (NBP1-30130) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Nephrin Antibody (NBP1-30130)

Discover related pathways, diseases and genes to Nephrin Antibody (NBP1-30130). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nephrin Antibody (NBP1-30130)

Discover more about diseases related to Nephrin Antibody (NBP1-30130).
 

Pathways for Nephrin Antibody (NBP1-30130)

View related products by pathway.

PTMs for Nephrin Antibody (NBP1-30130)

Learn more about PTMs related to Nephrin Antibody (NBP1-30130).

Blogs on Nephrin

There are no specific blogs for Nephrin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Evren Azeloglu
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NPHS1
Entrez
Uniprot