Nephrin Antibody (Y17-R)

Images

 
Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins. Representative image of Western blot for Nephrin antibody ...read more
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130] - Nephrin expression in HEK293 cells, visualized with clonal rabbit antiNephrin monospecific antibody. Primary antibody dilution - 1:70
Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, IP
Clone
Y17-R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nephrin Antibody (Y17-R) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from the center of the fifth Ig-like domain of human Nephrin. Antibody recognizes the epitope located between Cys465 - Cys528.
Epitope
Cys465 - Cys528
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NPHS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:50000-1:100000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID:34516901). For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS). ; 0.1% Triton X-100. Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk. Use in Immunohistochemistry reported in scientific literature (PMID: 25129444). .
Publications
Read Publications using
NBP1-30130 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Nephrin Antibody (Y17-R)

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nephrin Antibody (NBP1-30130)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-30130 Applications Species
Pace JA, Bronstein R, Guo Y et al. Podocyte-specific KLF4 is required to maintain parietal epithelial cell quiescence in the kidney Science advances Sep 3 2021 [PMID: 34516901] (IHC-P, Mouse) IHC-P Mouse
Melica ME, La Regina G, Parri M, et al. Substrate Stiffness Modulates Renal Progenitor Cell Properties via a ROCK-Mediated Mechanotransduction Mechanism Cells Dec 3 2019 [PMID: 31816967] (ICC/IF, Human) ICC/IF Human
Wang X, Gao Y, Tian N et al. Astragaloside IV improves renal function and fibrosis via inhibition of miR-21-induced podocyte dedifferentiation and mesangial cell activation in diabetic mice Drug Des Devel Ther Aug 6 2018 [PMID: 30122901] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Estrada CC, Paladugu P, Guo Y et al. Kruppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation JCI Insight Jun 21 2018 [PMID: 29925693] (ICC/IF, Mouse) ICC/IF Mouse
Hu M, Azeloglu EU, Ron A et al. A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep. Mar 6 2017 [PMID: 28262745] (WB, Human) WB Human
Mallipattu S K, Guo Y et al. Kruppel-Like Factor 15 Mediates Glucocorticoid-Induced Restoration of Podocyte Differentiation Markers. J Am Soc Nephrol Jan 1 2017 [PMID: 27288011] (IHC, Human, Mouse) IHC Human, Mouse
Finne K, Vethe H, Skogstrand T et al. Proteomic analysis of formalin-fixed paraffin-embedded glomeruli suggests depletion of glomerular filtration barrier proteins in two-kidney one-clip hypertensive rats. Nephrol. Dial. Transplant. 2014 Aug 16 [PMID: 25129444]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NPHS1
Entrez
Uniprot