Nephrin Antibody (G17-H)

Images

 
Western Blot: Nephrin Antibody (G17-H) [NBP1-30137] - Western Blot on recombinant protein. Lane 1 - 50ng; lane 2 - 100ng; Lane 3 - 200ng of recombinant mouse nephrin (extracellular domain, Gln 37 - Thr 1049).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IP
Clone
G17-H
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nephrin Antibody (G17-H) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from C-terminal sequence of first Ig-like domain of human nephrin. Antibody recognizes the epitope located between Cys53 - Cys101.
Specificity
This antibody is specific for the C terminus of first Ig-like domain of human nephrin (between residues Cys53-Cys101).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NPHS1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:20000-1:50000
  • Immunoprecipitation 1:10-1:500
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Tween-20. Blocking buffer: 1xTBS; 0.1% Tween-20; 8% nonfat dry milk.
Control
Nephrin Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Nephrin Antibody (G17-H)

  • CNF
  • Nephrin
  • nephrosis 1, congenital, Finnish type (nephrin)
  • NPHNCNF
  • NPHS1
  • Renal glomerulus-specific cell adhesion receptor

Background

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol NPHS1
Entrez
Uniprot
COVID-19 update