NDC80 Antibody (JE31-10)

Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: JE31-10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NDC80 Antibody (JE31-10) Summary

• Description: Novus Biologicals Rabbit NDC80 Antibody (JE31-10) (NBP3-32419) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human NDC80 aa 593-642 / 642. (Uniprot: O14777)
• Localization: Nucleus, Chromosome, centromere, kinetochore.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDC80
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NDC80 Antibody (JE31-10)

• HEC1KNTC2
• HECRetinoblastoma-associated protein HEC
• Highly expressed in cancer protein
• highly expressed in cancer, rich in leucine heptad repeats (yeast)
• highly expressed in cancer, rich in leucine heptad repeats
• hsHec1
• hsNDC80
• kinetochore associated 2
• Kinetochore protein Hec1
• kinetochore protein NDC80 homolog
• Kinetochore-associated protein 2
• NDC80 homolog, kinetochore complex component (S. cerevisiae)
• TID3

Background

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDC80