BCAS2 Antibody

Images

 
Biological Strategies: Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 ...read more
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Independent Antibodies: Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm-Or, Pm, ZeSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BCAS2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and the C-terminus (residue 225) of human Breast Cancer Amplified Sequence 2 using the numbering given in entry NP_005863.1 (GeneID 10286).
Predicted Species
Primate (100%), Zebrafish (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
BCAS2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-40681 in the following applications:

Publications
Read Publications using
NB110-40681 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAS2 Antibody

  • breast carcinoma amplified sequence 2
  • Breast carcinoma-amplified sequence 2
  • DAM1DNA amplified in mammary carcinoma 1 protein
  • pre-mRNA-splicing factor SPF27
  • Snt309
  • SPF27
  • spliceosome associated protein, amplified in breast cancer
  • Spliceosome-associated protein SPF 27

Background

BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-338
Species: Ha, Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, PLA, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, MiAr, WB
NBP2-67597
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
H00027349-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-67481
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-77197
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
NB100-294
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, MiAr, WB
NB100-558
Species: Hu
Applications: CHIP-SEQ, ChIP, IHC, IHC-P, IP, WB
NBP2-92632
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP1-06556
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
MAB6028
Species: Hu
Applications: WB
NBP2-67901
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31348
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00000699-D01P
Species: Hu, Mu
Applications: ICC/IF, PLA, WB
NBP1-32775
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
NBP3-17480
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-85249
Species: Hu, Mu
Applications: WB
NBP2-67753
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB

Publications for BCAS2 Antibody (NB110-40681)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB110-40681 Applications Species
Chang SW, Su CH, Chen HH et al. DDB2 is a novel AR interacting protein and mediates AR ubiquitination/degradation. Int J Biochem Cell Biol 2012 Nov [PMID: 22846800]
David CJ, Boyne AR, Millhouse SR, Manley JL. The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex. Genes Dev 25(9):972-983. 2011 May 1. [PMID: 21536736]
Goulielmaki E, Tsekrekou M, Batsiotos N et al. The Splicing Factor XAB2 interacts with ERCC1-XPF and XPG for RNA-loop processing during mammalian development bioRxiv Jul 21 2020 (WB, ICC/IF, Mouse) WB, ICC/IF Mouse

Review for BCAS2 Antibody (NB110-40681) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-40681:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IP Human 10/18/2010
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa NE
SpeciesHuman
LotA1
CommentsThis antibody is terrific for IP and western applications. 10 ug of this Spf27 antibody was able to deplete 60% of the Prp19C from 1 mg of extract.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 5 minutes, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: TBST 5% milk, Dilution Ratio: 1:2000, Incubation Time: 1 hour, Incubation Temp: 23 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Li-cor anti-rabbit

Details

Detection NotesSpecific Bands: 27kDa, Non-Specific Bands: none

Comments

CommentsThis antibody is terrific for IP and western applications. 10 ug of this Spf27 antibody was able to deplete 60% of the Prp19C from 1 mg of extract.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BCAS2 Antibody (NB110-40681) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BCAS2 Products

Bioinformatics Tool for BCAS2 Antibody (NB110-40681)

Discover related pathways, diseases and genes to BCAS2 Antibody (NB110-40681). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BCAS2 Antibody (NB110-40681)

Discover more about diseases related to BCAS2 Antibody (NB110-40681).
 

Pathways for BCAS2 Antibody (NB110-40681)

View related products by pathway.

PTMs for BCAS2 Antibody (NB110-40681)

Learn more about PTMs related to BCAS2 Antibody (NB110-40681).

Blogs on BCAS2

There are no specific blogs for BCAS2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Verified Customer
10/18/2010
Application: IP
Species: Human

Bioinformatics

Gene Symbol BCAS2
Entrez
Uniprot