BCAS2 Antibody

Images

 
Biological Strategies: Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 ...read more
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of Human BCAS2 on HeLa whole cell lysates using NB110-40681. BCAS2 was also immunoprecipitated by other rabbit anti-BCAS2 antibodies.
Independent Antibodies: Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, ZeSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

BCAS2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 175 and the C-terminus (residue 225) of human Breast Cancer Amplified Sequence 2 using the numbering given in entry NP_005863.1 (GeneID 10286).
Predicted Species
Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
BCAS2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-40681 in the following applications:

Publications
Read Publications using NB110-40681.

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAS2 Antibody

  • breast carcinoma amplified sequence 2
  • Breast carcinoma-amplified sequence 2
  • DAM1DNA amplified in mammary carcinoma 1 protein
  • pre-mRNA-splicing factor SPF27
  • Snt309
  • SPF27
  • spliceosome associated protein, amplified in breast cancer
  • Spliceosome-associated protein SPF 27

Background

BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCAS2 Antibody (NB110-40681)(2)

Review for BCAS2 Antibody (NB110-40681) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-40681:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IP Human 10/18/2010
View

Summary

ApplicationImmunoprecipitation
Sample TestedHeLa NE
SpeciesHuman
LotA1
CommentsThis antibody is terrific for IP and western applications. 10 ug of this Spf27 antibody was able to deplete 60% of the Prp19C from 1 mg of extract.

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 5 minutes, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: TBST 5% milk, Dilution Ratio: 1:2000, Incubation Time: 1 hour, Incubation Temp: 23 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Li-cor anti-rabbit

Details

Detection NotesSpecific Bands: 27kDa, Non-Specific Bands: none

Comments

CommentsThis antibody is terrific for IP and western applications. 10 ug of this Spf27 antibody was able to deplete 60% of the Prp19C from 1 mg of extract.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BCAS2 Antibody (NB110-40681) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Discover related pathways, diseases and genes to BCAS2 Antibody (NB110-40681). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on BCAS2

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Recent Reviews

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Anonymous
10/18/2010
Application: IP
Species: Human

Bioinformatics

Gene Symbol BCAS2
Entrez
Uniprot