Biological Strategies: Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 ...read more
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of Human BCAS2 on HeLa whole cell lysates using NB110-40681. BCAS2 was also immunoprecipitated by other rabbit anti-BCAS2 antibodies.
Independent Antibodies: Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more
The immunogen recognized by this antibody maps to a region between residue 175 and the C-terminus (residue 225) of human Breast Cancer Amplified Sequence 2 using the numbering given in entry NP_005863.1 (GeneID 10286).
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Immunogen affinity purified
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spliceosome associated protein, amplified in breast cancer
Spliceosome-associated protein SPF 27
BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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