NDC80 Antibodies
We offer NDC80 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each NDC80 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NDC80 Antibodies can be used in a variety of model species: C. elegans, Hamster, Human, Monkey, Mouse, Porcine, Rat. Use the list below to choose the NDC80 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NDC80 polyclonal antibodies and browse our NDC80 monoclonal antibody catalog.
Alternate Names for NDC80 Antibodies
anti-NDC80 antibody, anti-HEC1KNTC2 antibody, anti-HECRetinoblastoma-associated protein HEC antibody, anti-Highly expressed in cancer protein antibody, anti-highly expressed in cancer, rich in leucine heptad repeats (yeast) antibody, anti-highly expressed in cancer, rich in leucine heptad repeats antibody, anti-hsHec1 antibody, anti-hsNDC80 antibody, anti-kinetochore associated 2 antibody, anti-Kinetochore protein Hec1 antibody, anti-kinetochore protein NDC80 homolog antibody, anti-Kinetochore-associated protein 2 antibody, anti-NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, anti-TID3 antibody
10 Results for "ndc80" in Primary Antibodies
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0016.jpg "Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024205614.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-91020242024619.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024200109.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-910202420134060.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-western-blot-1411202518334521.jpg)
![Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NB100-1100-img0003.jpg "Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0002.jpg)
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0001.jpg)
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0003.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody")
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0002.jpg)
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13726_rabbit-ndc80-p-ser-55-pab-9102024205664.jpg)
![Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Western-Blot-H00010403-M01-img0004.jpg "Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.")
![Sandwich ELISA: NDC80 Antibody (1A10) [H00010403-M01] - Detection limit for recombinant GST tagged KNTC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Sandwich-ELISA-H00010403-M01-img0003.jpg)
![Western Blot: NDC80 Antibody [NBP2-56920] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NBP2-56920-img0002.jpg "Western Blot: NDC80 Antibody [NBP2-56920] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody [NBP2-56920] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56920-img0001.jpg)
![Western Blot: NDC80 Antibody (SR2187) [NBP3-21773] - Western blot analysis using NBP3-21773 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21773_rabbit-ndc80-mab-sr2187-57202315521811.jpg "Western Blot: NDC80 Antibody (SR2187) [NBP3-21773] - Western blot analysis using NBP3-21773 in Jurkat cell lysate.")
![Western Blot: NDC80 Antibody [NBP3-38149] - Western blot analysis of various lysates using NDC80 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38149_rabbit-ndc80-pab-1012202411364625.jpg "Western Blot: NDC80 Antibody [NBP3-38149] - Western blot analysis of various lysates using NDC80 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [HL3190] (NBP3-48778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48778_rabbit-ndc80-p-ser55-mab-hl3190-western-blot-1411202518252018.jpg "<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [HL3190] (NBP3-48778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121] - Western blot analysis of extracts of various cell lines, using NDC80 Rabbit mAb (NBP3-16121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/NDC80-Antibody-4W2Y2-Western-Blot-NBP3-16121-img0001.jpg "Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121] - Western blot analysis of extracts of various cell lines, using NDC80 Rabbit mAb (NBP3-16121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32419_rabbit-ndc80-mab-je31-10-278202415295591.jpg "Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")