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MIS12 Antibody

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Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody NB100-2359 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunohistochemistry: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (NB100-2359). ...read more
Independent Antibodies: Immunoprecipitation: MIS12 Antibody [NB100-2359] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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MIS12 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 205) of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MIS12
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:2500
Control
MIS12 Overexpression Lysate
Publications
Read Publications using NB100-2359.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MIS12 Antibody

  • 2510025F08Rik
  • hMis12
  • homolog of yeast Mis12
  • KNTC2AP
  • MGC2488
  • MIS12 homolog
  • MIS12, MIND kinetochore complex component, homolog (S. pombe)
  • MIS12, MIND kinetochore complex component, homolog (yeast)
  • MTW1
  • protein MIS12 homolog

Background

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and forkinetochore formation during mitosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CENPA Antibody [NBP2-92632] - Analysis of extracts of various cell lines, using CENPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.
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Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.
Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using HEC1(NB100-338) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
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Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASC5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: CASC5 Antibody [NB100-2586] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbitanti-CASC5 antibody used for IP at 6 ug per reaction. CASC5 was alsoimmunoprecipitated by a previous lot of this antibodyl. For blotting immunoprecipitated CASC5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: CASC5 Antibody [NB100-2586] - ASPP1/2 interact with multiple kinetochore components. Endogenous ASPP1/2 interact with multiple kinetochore components. Immunoprecipitation with anti-ASPP1 or ASPP2 antibodies were performed using cell lysates prepared from HeLa cells. The presence of kinetochore components in the immunoprecipitates was detected by WB analyses with their indicated antibodies. Image collected and cropped by CiteAb from the following publication (http://www.oncotarget.com/fulltext/6355), licensed under a CC-BY licence.
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Western Blot: CENPI Antibody [H00002491-B01P] - Analysis of CENPI expression in transfected 293T cell line by CENPI polyclonal antibody. Lane 1: FSHPRH1 transfected lysate(57.42 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CENPI Antibody [H00002491-B01P] - Analysis of purified antibody to FSHPRH1 on HeLa cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: ICC/IF, WB
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Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human skin shows weak to moderate nuclear positivity in a subset of squamous epithelial cells.
Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human small intestine shows weak to moderate nuclear positivity in a subset of glandular cells.
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Immunohistochemistry-Paraffin: DSN1 Antibody [NBP1-88302] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: DSN1 Antibody [NBP1-88302] - Staining in human testis and heart muscle tissues using anti-DSN1 antibody. Corresponding DSN1 RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: DC8 Antibody [NBP2-99483] - Immunofluorescence staining of DC8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DC8 polyclonal antibody (dilution ratio 1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .
Western Blot: DC8 Antibody [NBP2-99483] - Anti-DC8 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 34 kDa
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Immunohistochemistry-Paraffin: ZWINT Antibody [NBP1-82868] - Staining of human small intestine shows distinct cytoplasmic and membranous positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
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(8G1) - BSA Free
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Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.
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Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with rIgG (AF488) isotype control (blue).
Immunocytochemistry/Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.
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alpha-Defensin 1
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Immunocytochemistry/Immunofluorescence: SPC24 Antibody [NBP2-47264] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
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Western Blot: SPC24 Antibody [NBP2-47264] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
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Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.
Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control
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Bub1 Antibody
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Proximity Ligation Assay: Bub1 Antibody [H00000699-D01P] - Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in Jurkat.
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SPC25 Antibody
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Immunocytochemistry/Immunofluorescence: SPC25 Antibody [NBP2-47263] - Staining of human cell line U-251 MG shows localization to cytosol.
Western Blot: SPC25 Antibody [NBP2-47263] - Analysis in human cell line U-2 OS.
Immunohistochemistry-Paraffin: SPC25 Antibody [NBP2-47263] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
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Applications: ICC/IF, IHC, IHC-P, WB
CENPB Antibody
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Immunocytochemistry/Immunofluorescence: CENPB Antibody [H00001059-B01P] - Analysis of purified antibody to CENPB on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: CENPB Antibody [H00001059-B01P] - Analysis of CENPB expression in transfected 293T cell line by CENPB polyclonal antibody. Lane1:CENPB transfected lysate(65.89 KDa). Lane2:Non-transfected lysate.
Western Blot: CENPB Antibody [H00001059-B01P] - Analysis of CENPB expression in A-431.
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INCENP Antibody
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ELISA: INCENP Antibody (3D2) [NBP2-37471] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB

Publications for MIS12 Antibody (NB100-2359)(2)


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Control Lysate(s)

MIS12 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MIS12
Entrez
Uniprot