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MIS12 Antibody

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Western Blot: MIS12 Antibody [NB100-2359] - Detection of Human MIS12 on HeLa whole cell lysate using NB100-235. MIS12 was also immunoprecipitated by rabbit another anti-MIS12 antibody.
Independent Antibodies: Western Blot: MIS12 Antibody [NB100-2359] - Detection of Human MIS12 on HeLa whole cell lysate using NB100-235. MIS12 was also immunoprecipitated by rabbit another anti-MIS12 antibody.
Immunohistochemistry: MIS12 Antibody [NB100-2359] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-MIS12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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MIS12 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 205) of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MIS12
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2500
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
Positive Control
MIS12 Lysate (NBL1-13115)
Publications
Read Publications using NB100-2359.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MIS12 Antibody

  • 2510025F08Rik
  • hMis12
  • homolog of yeast Mis12
  • KNTC2AP
  • MGC2488
  • MIS12 homolog
  • MIS12, MIND kinetochore complex component, homolog (S. pombe)
  • MIS12, MIND kinetochore complex component, homolog (yeast)
  • MTW1
  • protein MIS12 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CENPA Antibody (5A7-2E11) [NBP2-12892] - analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.
Immunocytochemistry/Immunofluorescence: CENPA Antibody (5A7-2E11) [NBP2-12892] - Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
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NDC80 Antibody
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Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 protein stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (NBP2-21604) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
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Species: Hu, Mu, Rt, Po, Ha, Mk
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Western Blot: CASC5 Antibody [NB100-2586] - Detection of Human CASC5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASC5 antibody NB100-2586 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: CASC5 Antibody [NB100-2586] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CASC5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-CASC5 antibody NB100-2586 lot 2 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CASC5 was also immunoprecipitated by a previous lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
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NBP2-55474
Immunocytochemistry/Immunofluorescence: CENPC Antibody [NBP2-55474] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies, cytokinetic bridge, midbody, midbody ring & cleavage furrow.
Immunocytochemistry/Immunofluorescence: CENPC Antibody [NBP2-55474] - Staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.
Species: Hu
Applications: ICC/IF
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H00002491-B01P
Western Blot: CENPI Antibody [H00002491-B01P] - Analysis of CENPI expression in transfected 293T cell line by CENPI polyclonal antibody. Lane 1: FSHPRH1 transfected lysate(57.42 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CENPI Antibody [H00002491-B01P] - Analysis of purified antibody to FSHPRH1 on HeLa cell. (antibody concentration 10 ug/ml)
Species: Hu
Applications: WB, ICC/IF
CENPH Antibody
NBP1-82546
Western Blot: CENPH Antibody [NBP1-82546] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-119
Immunocytochemistry/Immunofluorescence: CENPH Antibody [NBP1-82546] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human small intestine shows distinct nuclear positivity in a subset of cells.
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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NBP1-88302
Western Blot: DSN1 Antibody [NBP1-88302] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: DSN1 Antibody [NBP1-88302] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
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Immunocytochemistry/Immunofluorescence: DC8 Antibody [NBP2-13678] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.
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Immunohistochemistry-Paraffin: DC8 Antibody [NBP2-13678] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells and strong nuclear staining in non-germinal center cells.
Species: Hu
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NB100-2364
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP, ICC, IF
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Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDCA1 protein stained by CDCA1 antibody [312] diluted at 1:200. Blue: Hoechst 33342 staining.
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Species: Hu
Applications: WB, ICC/IF
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H00151648-M01
Western Blot: Shugoshin Antibody (3C11) [H00151648-M01] - Analysis of SGOL1 expression in transfected 293T cell line by SGOL1 monoclonal antibody (M01), clone 3C11.Lane 1: SGOL1 transfected lysate(33.5 KDa).Lane 2: Non-transfected lysate.
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Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.
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Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry: HP1 alpha Antibody [NB110-40623] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - FFPE section of human ovarian tumor. Affinity purified rabbit anti-BIRC6/Apollon dilution of 1:250.
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alpha-Defensin 1
Antibody [Unconjugate ...
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Species: Hu
Applications: ELISA, IHC
SPC24 Antibody
NBP2-47264
Western Blot: SPC24 Antibody [NBP2-47264] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/Immunofluorescence: SPC24 Antibody [NBP2-47264] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunohistochemistry: SPC24 Antibody [NBP2-47264] - Staining of human testis shows moderate nuclear positivity in cells in subset of cells in seminiferus ducts.
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Aurora B Antibody
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Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB100-294] - RPE1 cells stained with Aurora B antibody (green). Image from verified customer review.
Immunohistochemistry: Aurora B Antibody [NB100-294] - Rabbit polyclonal to Aurora B (NB100-294) used to stain SW620 human tumour xenografts (in mouse). The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
Chromatin Immunoprecipitation: Aurora B Antibody [NB100-294] - 293T cells stained with Aurora B antibody. Image from verified customer review.
(5 Reviews)
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Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.
Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in Jurkat.
Species: Hu, Mu
Applications: WB, ICC/IF, PLA
SPC25 Antibody
NBP2-47263
Western Blot: SPC25 Antibody [NBP2-47263] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/Immunofluorescence: SPC25 Antibody [NBP2-47263] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: SPC25 Antibody [NBP2-47263] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
CENPB Antibody
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Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Detection of Human CENP-B by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B (NB100-2369) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.
Immunohistochemistry: CENPB Antibody [NB100-2369] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.
Immunocytochemistry/Immunofluorescence: CENPB Antibody [NB100-2369] - Detection of Human CENP-B by Immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-B (NB100-2369) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (NB7159) used at a dilution of 1:100.
Species: Hu
Applications: ICC/IF, IHC
INCENP Antibody
(3D2)
NBP2-37471
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: INCENP Antibody (3D2) [NBP2-37471] - Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: INCENP Antibody (3D2) [NBP2-37471] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

Publications for MIS12 Antibody (NB100-2359)(2)


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Positive Control Lysate(s)

MIS12 Overexpression Lysate (Native)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MIS12
Entrez
Uniprot