CENPH Antibody

Western Blot: CENPH Antibody [NBP1-82546] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-119

Immunocytochemistry/Immunofluorescence: CENPH Antibody [NBP1-82546] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human small intestine shows weak to moderate nuclear positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human tonsil shows weak to moderate nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: CENPH Antibody [NBP1-82546] - Staining of human skin shows weak to moderate nuclear positivity in a subset of squamous epithelial cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CENPH Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CENPH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CENPH Protein (NBP1-82546PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CENPH Antibody

• CENP-H
• centromere protein H
• ICEN35
• Interphase centromere complex protein 35
• kinetochore protein CENP-H
• NNF1
• PMF1

Background

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CENPH Antibody (NBP1-82546)(1)

Publication using NBP1-82546

• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: CENPH