MAD2L1 Antibody

Images

 
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

MAD2L1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAD2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-4 ug/mg lysate
  • Western Blot 1:100-1:2000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. Use in ICC/IF was reported in scientific literature (PMID: 29497093).
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
MAD2L1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-563 in the following applications:

Publications
Read Publications using
NB100-563 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAD2L1 Antibody

  • HSMAD2
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2L1
  • MAD2-like protein 1
  • MAD2mitotic arrest deficient, yeast, homolog-like 1
  • Mitotic arrest deficient 2-like protein 1
  • mitotic spindle assembly checkpoint protein MAD2A

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAD2L1 Antibody (NB100-563)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-563 Applications Species
Itoh Go, Ikeda Masanori, Iemura Kenji et al. Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment. Medicine 2018-03-01 [PMID: 29497093] (ICC/IF, Human) ICC/IF Human
Ikeda M, Tanaka K. Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis Sci Rep 2017-08-18 [PMID: 28821799] (WB, Human) WB Human
Guccini S, Lombardi S, Pisani A et al. Effects of spindle poisons in peripheral human lymphocytes by the in vitro cytokinesis-block micronucleus assay. Mutagenesis 2012-11-01 [PMID: 22952150]
Matson DR, Demirel PB, Stukenberg PT, Burke DJ. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. FEBS J. 2012-03-16 [PMID: 22426531]
McHedlishvili N, Wieser S, Holtackers R et al. Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. J Cell Sci 2012-02-01 [PMID: 22399803]
Klebig C, Korinth D, Meraldi P et al. Bub1 regulates chromosome segregation in a kinetochore-independent manner. J Cell Biol 2009-06-01 [PMID: 19487456]
Chan YW, Fava LL, Uldschmid A et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009-06-01 [PMID: 19468067]
Yun M, Han YH, Yoon SH et al. p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption. Mol Cancer Res 2009-03-01 [PMID: 19276188]
Lince-Faria M, Maffini S, Orr B et al. Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol 2009-03-01 [PMID: 19273613]
Vader G, Cruijsen CW, van Harn T et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Mol Biol Cell 2007-11-01 [PMID: 17699588]
Show All 14 Publications.

Review for MAD2L1 Antibody (NB100-563) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-563:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MAD2L1 NB100-563
Enlarge
4
reviewed by:
Verified Customer
WB Human 08/27/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAD2L1 Antibody (NB100-563) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
08/27/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAD2L1
Entrez
Uniprot