MAD2L1 Antibody

Images

 
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MAD2L1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAD2L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-4 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
MAD2L1 Lysate (NBL1-12786)
Reviewed Applications
Read 1 Review rated 4
using
NB100-563 in the following applications:

Publications
Read Publications using
NB100-563 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAD2L1 Antibody

  • HSMAD2
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2L1
  • MAD2-like protein 1
  • MAD2mitotic arrest deficient, yeast, homolog-like 1
  • Mitotic arrest deficient 2-like protein 1
  • mitotic spindle assembly checkpoint protein MAD2A

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAD2L1 Antibody (NB100-563)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-563 Applications Species
Ikeda M, Tanaka K. Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis Sci Rep 2017 Aug 18 [PMID: 28821799] (WB, Human) WB Human
Guccini S, Lombardi S, Pisani A et al. Effects of spindle poisons in peripheral human lymphocytes by the in vitro cytokinesis-block micronucleus assay. Mutagenesis 2012 Nov [PMID: 22952150]
Matson DR, Demirel PB, Stukenberg PT et al. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev 2012 Mar [PMID: 22426531]
McHedlishvili N, Wieser S, Holtackers R et al. Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. J Cell Sci 2012 Feb [PMID: 22399803]
Klebig C, Korinth D, Meraldi P et al. Bub1 regulates chromosome segregation in a kinetochore-independent manner. J Cell Biol 2009 Jun [PMID: 19487456]
Chan YW, Fava LL, Uldschmid A et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol 2009 Jun [PMID: 19468067]
Yun M, Han YH, Yoon SH et al. p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption. Mol Cancer Res 2009 Mar [PMID: 19276188]
Lince-Faria M, Maffini S, Orr B et al. Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol 2009 Mar [PMID: 19273613]
Vader G, Cruijsen CW, van Harn T et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Mol Biol Cell 2007 Nov [PMID: 17699588]
Santamaria A, Neef R, Eberspacher U et al. Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis. Mol Biol Cell 2007 Oct [PMID: 17671160]
Show All 13 Publications.

Review for MAD2L1 Antibody (NB100-563) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-563:
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WB
(1)
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Human
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Images Ratings Applications Species Date Details
Western Blot MAD2L1 NB100-563
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reviewed by:
Anonymous
WB Human 08/27/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from HeLa cells
SpeciesHuman
CommentsThe blot also shows a band of higher molecular weight, consistent with the blots shown on the website.

Blocking

Blocking Details5% NFDM in PBS+0.1% Triton

Primary Anitbody

Dilution Ratio2000 times diluted in PBS, incubated for 2 hours at room temperature

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP-linked Antibody (Cell Signaling)
Secondary Manufacturer Cat##7074
Secondary Concentration20,000

Details

Detection NotesCaptured using X-rays (5 sec exposure) with Phototope-HRP Western Blot Detection System

Comments

CommentsThe blot also shows a band of higher molecular weight, consistent with the blots shown on the website.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Anonymous
08/27/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAD2L1
Entrez
Uniprot