Aurora B Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB100-294] - RPE1 cells stained with Aurora B antibody (green). Image from verified customer review.
Immunohistochemistry: Aurora B Antibody [NB100-294] - Rabbit polyclonal to Aurora B (NB100-294) used to stain SW620 human tumour xenografts (in mouse). The sections were microwave pretreated in citrate buffer (pH ...read more
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB100-294] - IF analysis fo Aurora B in RPE-1 cells. Verified customer review from 1DegreeBio.
Immunofluorescence: Aurora B Antibody [NB100-294] - 293T cells stained with Aurora B antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, MiAr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
12.5 mg/ml

Order Details

Aurora B Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.
Marker
Mitosis Marker
Specificity
Aurora B
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 60 ug / 300 ug lysate
  • Microarray
Application Notes
By Western blot, it detects a band of approximately 39 kDa.
Positive Control
Aurora B Lysate (NBL1-07860)
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB100-294 in the following applications:

Publications
Read Publications using
NB100-294 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
12.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aurora B Antibody

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora B Antibody (NB100-294)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


Filter By Application
ICC/IF
(4)
IHC
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-294 Applications Species
Sijare F, Geibler AL, Fichter CD et al. Aurora B expression and histone variant H1.4S27 phosphorylation are no longer coordinated during metaphase in aneuploid colorectal carcinomas Virchows Arch. 2015 Feb 14 [PMID: 25680570] (ICC/IF) ICC/IF
Kleyman M, Kabeche L, Compton DA. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J. Cell. Sci. 2014 Jul 29 [PMID: 25074805] (ICC/IF, Human)

Details:
PFA fixed cells permeabilized with -20C methanol for 5 min, incubated wit primary for 1 hour RT in TBS-BSA plus 0.1% Triton X-100. Figure 3A - Aurora B immunostaining in HCT116 cells Control or transfected with STAG2 siRNA.
ICC/IF Human
Kabeche L, Compton DA. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013 Oct 3 [PMID: 24013174] (ICC/IF, Human) ICC/IF Human
Kabeche L, Compton DA. Checkpoint-Independent Stabilization of Kinetochore-Microtubule Attachments by Mad2 in Human Cells Current Biology 2012 Apr 10 [PMID: 22405866] (ICC/IF, Human) ICC/IF Human
Beussel S, Hasenburg A, Bogatyreva L, Hauschke D, Werner M, Lassmann S. Aurora-B protein expression is linked to initial response to taxane-based first-line chemotherapy in stage III ovarian carcinoma. J Clin Pathol;65(1):29-35. 2012 Jan. [PMID: 22011448] (IHC, Human) IHC Human
Bufo P, Sanguedolce F, Tortorella S et al. Expression of mitotic kinases phospho-aurora A and aurora B correlates with clinical and pathological parameters in bladder neoplasms. Histol Histopathol;25(11):1371-7. 2010 Nov. [PMID: 20865660]
Lee, E C Y, Frolov, A, Li, R, Ayala, G, Greenberg, N M. Targeting Aurora Kinases for the Treatment of Prostate Cancer. Can Res 66: 4996-5002. 2006 [PMID: 16707419]

Reviews for Aurora B Antibody (NB100-294) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.
We have 5 reviews tested in 3 application: IF, IHC-P, ICC.

Reviews using NB100-294:
Filter by Applications
IF
(3)
IHC-P
(1)
ICC
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Aurora B NB100-294
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reviewed by:
Anonymous
IF Human 04/21/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE1 cells
SpeciesHuman

Blocking

Blocking DetailsBlocked in PBS-BSA (1% BSA) for 10 minutes

Primary Anitbody

Dilution RatioAntibody was diluted in PBS-BSA (1%BSA) at 1:1000 and incubated for 1hr at room temperature

Secondary Antibody

Secondary DescriptionDonkey anti-rabit Alexafluor 488
Secondary Manufacturer Cat#Jackson Immuno Laboratory
Secondary ConcentrationAntibody was diulted at 1:1000 in PBS-BSA (1%BSA) for 1hr at room temperature

Details

Detection NotesNikon TiE was used for imaging. Single focal plane image at 60X
Fixation DetailsFixed with 3.5% paraformaldehyde for 15 minuted. Permeabilized with PBS+0.5% TritonX100 for 5 minutes. Washed in PBS for 5mins
Wash Description1 Wash in PBS for 5 minutes

Comments

CommentsDNA was stained using DAPI in the secondary antibody incubation
Immunohistochemistry-Paraffin Aurora B NB100-294
Enlarge
reviewed by:
Anonymous
IHC-P Human 10/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested293T cell line
SpeciesHuman
Immunocytochemistry Aurora B NB100-294
Enlarge
reviewed by:
Andrew Cosgrove
ICC Human 11/06/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedRPE-1 cells
SpeciesHuman

Primary Anitbody

Dilution Ratio1:1000 in TBS-BSA + 0.1% TritonX for 1 hr

Secondary Antibody

Secondary ConcentrationIn TBS-BSA + 0.1% TritonX for 1hr

Details

Fixation DetailsFixed in 3.5% Paraformaldehyde
Wash DescriptionWash with TBS-BSA + 0.5% TritonX for 5 mins
 
reviewed by:
Anonymous
IF Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
FileView PDF
 
reviewed by:
Duane Compton
IF Human 05/02/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE-1
SpeciesHuman
Lot043K4827

Primary Anitbody

Dilution Ratio1:1,000 Dilution of Ab in TBS-BSA + 0.1% Triton X for 1hr

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG
Secondary Manufacturer Cat#F1-1000
Secondary Concentration1:2,000

Details

Fixation DetailsFixed with 3.5% Paraformaldehyde for 15 minutes. Washed with TBS-BSA + 0.5% TritonX for 5 minutes. Washed with TBS-BSA for 5 min
Wash DescriptionWashed with TBS-BSA for 5 minutes with gentle shaking (1 time)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aurora B Antibody (NB100-294) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Aurora B Antibody (NB100-294)

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Anonymous
04/21/2016
Application: IF
Species: Human

Anonymous
10/02/2015
Application: IHC-P
Species: Human

Andrew Cosgrove
11/06/2013
Application: ICC
Species: Human

Bioinformatics

Gene Symbol AURKB
Entrez