Aurora B Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB100-294] - RPE1 cells stained with Aurora B antibody (green). Image from verified customer review.
Immunohistochemistry: Aurora B Antibody [NB100-294] - Rabbit polyclonal to Aurora B (NB100-294) used to stain SW620 human tumour xenografts (in mouse). The sections were microwave pretreated in citrate buffer (pH ...read more
Chromatin Immunoprecipitation: Aurora B Antibody [NB100-294] - 293T cells stained with Aurora B antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody [NB100-294] - IF analysis fo Aurora B in RPE-1 cells. Verified customer review from 1DegreeBio.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, MiAr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
12.5 mg/ml

Aurora B Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.
Marker
Mitosis Marker
Specificity
Aurora B
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AURKB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 60 ug / 300 ug lysate
  • Microarray
  • Western Blot 1:1000-1:2000
Application Notes
By Western blot, it detects a band of approximately 39 kDa.
Control
Aurora B Overexpression Lysate
Reviewed Applications
Read 5 Reviews rated 4.8
using
NB100-294 in the following applications:

Publications
Read Publications using
NB100-294 in the following applications:

  • 6 publications
  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
12.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aurora B Antibody - BSA Free

  • Aik2
  • AIK2EC 2.7.11.1
  • AIM1
  • AIM-1
  • AIM1Aik2
  • AIM-1STK-1
  • ARK2
  • ARK2ARK-2
  • AurB
  • AURKB
  • aurkb-sv2
  • Aurora- and IPL1-like midbody-associated protein 1
  • Aurora B
  • aurora kinase BSerine/threonine-protein kinase aurora-B
  • aurora kinase B-Sv1
  • aurora kinase B-Sv2
  • Aurora/IPL1-related kinase 2
  • aurora-1
  • aurora-B
  • Aurora-related kinase 2
  • EC 2.7.11
  • IPL1
  • serine/threonine kinase 12
  • serine/threonine-protein kinase 12
  • STK12
  • STK12aurkb-sv1
  • STK5

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora B Antibody (NB100-294)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(6)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-294 Applications Species
Chang X, Zhang T, Wang Q et al. HI-511 overcomes melanoma drug resistance via targeting AURKB and BRAF V600E Theranostics Aug 1 2020 [PMID: 32863956] (Human) Human
Fang H, Niu K, Mo D et al. RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity. Oncogenesis Sep 12 2018 [PMID: 30206236] (ICC/IF, Human) ICC/IF Human
Dumitru AMG, Rusin SF, Clark AEM et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability Elife. 2017 Nov 20 [PMID: 29154753] (ICC/IF, WB, Human) ICC/IF, WB Human
Sijare F, Geibler AL, Fichter CD et al. Aurora B expression and histone variant H1.4S27 phosphorylation are no longer coordinated during metaphase in aneuploid colorectal carcinomas Virchows Arch. 2015 Feb 14 [PMID: 25680570] (ICC/IF) ICC/IF
Kleyman M, Kabeche L, Compton DA. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J. Cell. Sci. 2014 Jul 29 [PMID: 25074805] (ICC/IF, Human)

Details:
PFA fixed cells permeabilized with -20C methanol for 5 min, incubated wit primary for 1 hour RT in TBS-BSA plus 0.1% Triton X-100. Figure 3A - Aurora B immunostaining in HCT116 cells Control or transfected with STAG2 siRNA.
ICC/IF Human
Kabeche L, Compton DA. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013 Oct 3 [PMID: 24013174] (ICC/IF, Human) ICC/IF Human
Kabeche L, Compton DA. Checkpoint-Independent Stabilization of Kinetochore-Microtubule Attachments by Mad2 in Human Cells Current Biology 2012 Apr 10 [PMID: 22405866] (ICC/IF, Human) ICC/IF Human
Beussel S, Hasenburg A, Bogatyreva L, Hauschke D, Werner M, Lassmann S. Aurora-B protein expression is linked to initial response to taxane-based first-line chemotherapy in stage III ovarian carcinoma. J Clin Pathol;65(1):29-35. 2012 Jan. [PMID: 22011448] (IHC, Human) IHC Human
Bufo P, Sanguedolce F, Tortorella S et al. Expression of mitotic kinases phospho-aurora A and aurora B correlates with clinical and pathological parameters in bladder neoplasms. Histol Histopathol;25(11):1371-7. 2010 Nov. [PMID: 20865660]
Chang X Hi-511 Inhibits Both BRAF and AURKB to Overcome Malignant Melanoma Drug Resistance and Metastasis Thesis Jan 1 2020
Show All 11 Publications.

Reviews for Aurora B Antibody (NB100-294) (5) 4.85

Average Rating: 4.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using NB100-294:
Filter by Applications
Immunofluorescence
(3)
Immunohistochemistry-Paraffin
(1)
Immunocytochemistry
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Aurora B NB100-294
Enlarge
5
reviewed by:
Lilian Kabeche
Immunofluorescence Human 04/21/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE1 cells
SpeciesHuman
Immunohistochemistry-Paraffin Aurora B NB100-294
Enlarge
5
reviewed by:
Yves Heremans
Immunohistochemistry-Paraffin Human 10/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested293T cell line
SpeciesHuman
Immunocytochemistry Aurora B NB100-294
Enlarge
4
reviewed by:
Verified Customer
Immunocytochemistry Human 11/06/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedRPE-1 cells
SpeciesHuman
  5
reviewed by:
Duane Compton
Immunofluorescence Human 10/04/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
  5
reviewed by:
Verified Customer
Immunofluorescence Human 05/02/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedRPE-1
SpeciesHuman
Lot043K4827

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Aurora B Antibody (NB100-294) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Aurora B Antibody (NB100-294)

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Contact Information

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Calculators

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Recent Reviews

4.8
5
4
4
1
3
0
2
0
1
0

Lilian Kabeche
04/21/2016
Application: Immunofluorescence
Species: Human

Yves Heremans
10/02/2015
Application: Immunohistochemistry-Paraffin
Species: Human

Verified Customer
11/06/2013
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol AURKB
Entrez