Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control

Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 12.5 mg/ml

Aurora B Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4
• Marker: Mitosis Marker
• Specificity: Aurora B
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AURKB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 50-60 ug
  • Microarray
  • Western Blot 1:1000-1:2000
• Application Notes: By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Aurora B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 12.5 mg/ml
• Purity: IgG purified

Alternate Names for Aurora B Antibody - BSA Free

• Aik2
• AIK2EC 2.7.11.1
• AIM1
• AIM-1
• AIM1Aik2
• AIM-1STK-1
• ARK2
• ARK2ARK-2
• AurB
• AURKB
• aurkb-sv2
• Aurora- and IPL1-like midbody-associated protein 1
• Aurora B
• aurora kinase BSerine/threonine-protein kinase aurora-B
• aurora kinase B-Sv1
• aurora kinase B-Sv2
• Aurora/IPL1-related kinase 2
• aurora-1
• aurora-B
• Aurora-related kinase 2
• EC 2.7.11
• IPL1
• serine/threonine kinase 12
• serine/threonine-protein kinase 12
• STK12
• STK12aurkb-sv1
• STK5

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aurora B Antibody (NB100-294)(11)

Publications using NB100-294

• Chang X, Zhang T, Wang Q et al. HI-511 overcomes melanoma drug resistance via targeting AURKB and BRAF V600E Theranostics 2020-08-01 [PMID: 32863956] (Human)
• Fang H, Niu K, Mo D et al. RecQL4-Aurora B kinase axis is essential for cellular proliferation, cell cycle progression, and mitotic integrity. Oncogenesis 2018-09-12 [PMID: 30206236] (ICC/IF, Human)
• Dumitru AMG, Rusin SF, Clark AEM et al. Cyclin A/Cdk1 modulates Plk1 activity in prometaphase to regulate kinetochore-microtubule attachment stability Elife. 2017-11-20 [PMID: 29154753] (ICC/IF, WB, Human)
  
  Details:
  HEC1 IF antibody used at a dilution of 1:500
• Sijare F, Geibler AL, Fichter CD et al. Aurora B expression and histone variant H1.4S27 phosphorylation are no longer coordinated during metaphase in aneuploid colorectal carcinomas Virchows Arch. 2015-02-14 [PMID: 25680570] (ICC/IF)
• Kleyman M, Kabeche L, Compton DA. STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments. J. Cell. Sci. 2014-07-29 [PMID: 25074805] (ICC/IF, Human)
  
  Details:
  Outer kinetochore component Hec1/NDC80 antibody used in ICC-IF on HCT116 and RPE1 cells for measuring centromere stretch (Fig. 1D, supplementary material Fig. S2A)
• Kabeche L, Compton DA. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013-10-03 [PMID: 24013174] (ICC/IF, Human)
• Kabeche L, Compton DA. Checkpoint-independent stabilization of kinetochore-microtubule attachments by Mad2 in human cells. Curr Biol 2012-04-01 [PMID: 22405866] (ICC/IF, Human)
• Beussel S, Hasenburg A, Bogatyreva L, Hauschke D, Werner M, Lassmann S. Aurora-B protein expression is linked to initial response to taxane-based first-line chemotherapy in stage III ovarian carcinoma. J Clin Pathol;65(1):29-35. 2012-01-01 [PMID: 22011448] (IF/IHC, Human)
• Bufo P, Sanguedolce F, Tortorella S et al. Expression of mitotic kinases phospho-aurora A and aurora B correlates with clinical and pathological parameters in bladder neoplasms. Histol Histopathol;25(11):1371-7. 2010-11-01 [PMID: 20865660]
• Chang X Hi-511 Inhibits Both BRAF and AURKB to Overcome Malignant Melanoma Drug Resistance and Metastasis Thesis 2020-01-01
• Lee EC, Frolov A, Li R et al. Targeting Aurora kinases for the treatment of prostate cancer. Cancer Res 2006-05-01 [PMID: 16707419]

Reviews for Aurora B Antibody (NB100-294) (5)

Average Rating: 4.8

(Based on 5 reviews)

We have 5 reviews tested in 1 species: Human.

reviewed by: Lilian Kabeche IF Human 04/21/2016

Summary

• Application: Immunofluorescence
• Sample Tested: RPE1 cells
• Species: Human

reviewed by: Yves Heremans IHC-P Human 10/02/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: 293T cell line
• Species: Human

reviewed by: Verified Customer ICC Human 11/06/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: RPE-1 cells
• Species: Human

reviewed by: Duane Compton IF Human 10/04/2013

Summary

• Application: Immunofluorescence
• Species: Human

reviewed by: Verified Customer IF Human 05/02/2012

Summary

• Application: Immunofluorescence
• Sample Tested: RPE-1
• Species: Human
• Lot: 043K4827

Bioinformatics

• Gene Symbol: AURKB
• Entrez:
  • 9212(Human)
  • 20877(Mouse)
  • 114592(Rat)