Nuf2 Antibody

Images

 
Western Blot: Nuf2 Antibody [NBP1-31632] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 10% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632] - Paraformaldehyde-fixed U2OS, using CDCA1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) ...read more
Western Blot: Nuf2 Antibody [NBP1-31632] - A431 diluted at 1:3000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Nuf2 Antibody Summary

Immunogen
The exact sequence is proprietary.
Localization
Nucleus, Kinetochore
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Nuf2 Antibody

  • cancer/testis antigen 106
  • CDCA1hsNuf2
  • cell division cycle associated 1
  • Cell division cycle-associated protein 1
  • CT106
  • hNuf2
  • kinetochore protein Nuf2
  • NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
  • NUF2RhNuf2R

Background

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NUF2