TTK Antibody (689913)

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Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TTK Monoclonal Antibody (Catalog # MAB6028) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
689913
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TTK Antibody (689913) Summary

Immunogen
E. coli-derived recombinant human TTK
Ser281-Leu481
Accession # P33981
Specificity
Detects human TTK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CaM Kinase II, rhCAM Kinase II alpha , rhCaM Kinase II beta , rhCaM Kinase IIδ, rhCaM Kinase II gamma , rhCaM Kinase III, rhCaM Kinase IV, rhCaM Kinase K alpha , or rhCaM Kinase K beta is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TTK
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB6028 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TTK Antibody (689913)

  • CT96
  • dual specificity protein kinase TTK
  • EC 2.7.12.1
  • ESK
  • FLJ38280
  • monopolar spindle 1-like 1
  • Mps1
  • MPS1L1
  • MPS1L1PYTcancer/testis antigen 96
  • phosphotyrosine picked threonine kinase
  • Phosphotyrosine picked threonine-protein kinase
  • PYT
  • TTK protein kinase
  • TTK

Background

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in testis and thymus, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length. It contains one protein kinase domain (aa 525‑791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. There are two potential splice variants. One shows an alternative start site at Met17, while another shows a deletion of Gln420. Over aa 281-481, human TTK shares 53% aa identity with mouse TTK.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB6028
Species: Hu
Applications: WB

Publications for TTK Antibody (MAB6028) (0)

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Review for TTK Antibody (MAB6028) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6028:
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Western Blot
(1)
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Images Ratings Applications Species Date Details
Western Blot TTK MAB6028
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4
reviewed by:
Lei Nie
Western Blot Human 04/04/2020
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Summary

ApplicationWestern Blot
Sample TestedHepG2
SpeciesHuman
LotCEUI011807A

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Recent Reviews

4
5
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4
1
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0
2
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1
0

Lei Nie
04/04/2020
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol TTK
Uniprot