MAD1L1/MAD1 Antibody

Images

 
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.
Immunocytochemistry/ Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a ...read more
Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract ...read more
Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MAD1L1/MAD1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.
Localization
Nucleus, Kinetochore
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAD1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32775 in the following applications:

  • 2 publications
  • 2 publications
  • WB
    2 publications

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1XPBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAD1L1/MAD1 Antibody

  • hMAD1
  • HsMAD1
  • MAD1 mitotic arrest deficient-like 1 (yeast)
  • MAD1
  • MAD1L1
  • MAD1-like protein 1
  • MAD1yeast, homolog-like 1
  • Mitotic checkpoint MAD1 protein homolog
  • PIG9
  • Tax-binding protein 181
  • TP53I9
  • tumor protein p53 inducible protein 9
  • TXBP181
  • TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MAD1L1/MAD1 Antibody (NBP1-32775)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, ICC/IF, WB.


Filter By Application
ChIP
(2)
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAD1L1
Entrez