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CASC5 Antibody

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Biological Strategies: Western Blot: CASC5 Antibody [NB100-2586] - ASPP1/2 interact with multiple kinetochore components. Endogenous ASPP1/2 interact with multiple kinetochore components. Immunoprecipitation with ...read more
Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CASC5 antibody used for WB at 0.1 ...read more
Immunoprecipitation: CASC5 Antibody [NB100-2586] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbitanti-CASC5 ...read more
ASPP1/2 interact with multiple kinetochore components c). Endogenous ASPP1/2 interact with multiple kinetochore components. Immunoprecipitation with anti-ASPP1 or ASPP2 antibodies were performed using cell lysates ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

CASC5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KNL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 29497093).
Publications
Read Publications using
NB100-2586 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CASC5 Antibody

  • AF15Q14
  • Blinkin
  • blinkin, bub-linking kinetochore protein
  • Bub-linking kinetochore protein
  • cancer susceptibility candidate 5
  • Cancer susceptibility candidate gene 5 protein
  • Cancer/testis antigen 29
  • CT29KIAA1570
  • D40
  • hKNL-1
  • hSpc105
  • kinetochore null 1 homolog
  • Kinetochore-null protein 1
  • KNL1
  • KNL1ALL1-fused gene from chromosome 15q14 protein
  • protein CASC5
  • Protein D40/AF15q14

Background

Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CASC5 Antibody (NB100-2586)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CASC5 Antibody (NB100-2586) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CASC5 Products

Array NB100-2586

Research Areas for CASC5 Antibody (NB100-2586)

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Blogs on CASC5

There are no specific blogs for CASC5, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol KNL1
Entrez
Uniprot