CASC5 Antibody

Images

 
Biological Strategies: Western Blot: CASC5 Antibody [NB100-2586] - Detection of Human CASC5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry: CASC5 Antibody [NB100-2586] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CASC5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Biological Strategies: Western Blot: CASC5 Antibody [NB100-2586] - Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) ...read more
Immunohistochemistry-Paraffin: CASC5 Antibody [NB100-2586] - FFPE section of human lung carcinoma. Affinity purified rabbit anti-CASC5 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: CASC5 Antibody [NB100-2586] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

CASC5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human Cancer Susceptibility Candidate 5 using the numbering given in entry NP_733468.2
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Publications
Read Publication using NB100-2586.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CASC5 Antibody

  • AF15Q14
  • Blinkin
  • blinkin, bub-linking kinetochore protein
  • Bub-linking kinetochore protein
  • cancer susceptibility candidate 5
  • Cancer susceptibility candidate gene 5 protein
  • Cancer/testis antigen 29
  • CT29KIAA1570
  • D40
  • hKNL-1
  • hSpc105
  • kinetochore null 1 homolog
  • Kinetochore-null protein 1
  • KNL1ALL1-fused gene from chromosome 15q14 protein
  • protein CASC5
  • Protein D40/AF15q14

Background

Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CASC5 Antibody (NB100-2586)(1)

We have publications tested in 1 application: IP.


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(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Additional Array Products

Array NB100-2586

Bioinformatics Tool for CASC5 Antibody (NB100-2586)

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Diseases for CASC5 Antibody (NB100-2586)

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PTMs for CASC5 Antibody (NB100-2586)

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Bioinformatics

Gene Symbol CASC5
Entrez
Uniprot