N-Cadherin Antibody (GC-4)


There are currently no images for N-Cadherin Antibody (NBP2-21805).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr

Order Details

View Available Formulations
Catalog# & Formulation Size Price

N-Cadherin Antibody (GC-4) Summary

Affinity purified chicken heart A-CAM.
Mesenchymal Cells Marker
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 10-20ug/ml
  • Electron Microscopy
  • Flow Cytometry 1:50-1:250
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 10-20ug/ml
Application Notes
Immunohistochemistry: 10-20 ug/mL using rat cardiac muscle frozen sections, Western blot: 10-20 ug/mL using chicken cardiac muscle or COS-7 cell extracts
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
N-Cadherin Lysate (NBP2-66344)

Reactivity Notes

chicken, monkey, rabbit, human, mouse, rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
10mM PBS (pH 7.4)
No Preservative
Protein A or G purified

Alternate Names for N-Cadherin Antibody (GC-4)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin


Clone GC-4 is also known as GB-9. Monoclonal Anti-N-Cadherin (mouse IgG1 isotype) is derived from the GC-4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with affinity purified chicken heart A-CAM. 1 The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-N-Cadherin/A-CAM is specific for the N-Cadherin. The antibody reacts with the N-terminal half of the extracellular domain of N-Cadherin. 1, 2 It recognizes a polypeptide of 135 kDa isolated from a freshly prepared extract chicken cardiac muscle by immunoblotting. This antibody can work as a neutralizing antibody that can inhibit adherens junction formation, 3 colony formation, cell adhesion, interaction between bone marrow hematopoietic cells, 4 and cytoskeleton formation. 5 Monoclonal Anti-N-Cadherin reacts with the N-Cadherin molecule from human, 4, 5 monkey, 6 rabbit, rat, 3 mouse, 7 and chicken. 1-3 The antibody may be used in various immunochemical techniques including immunohistochemistry, immunocytochemistry, 3, 6 electron microscopy, immunoblotting, 8 and FACS analysis. 4, 9 N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin was cloned from neural tissues but later on found to be expressed mainly in endothelial and muscle tissues. Like the other classical cadherin proteins (E- and P-cadherin), N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. 10-12 This protein mediates the intercellular adhesion by homophilic interaction between the extracellular domains of the cadherin proteins in neighboring cells. The cytoplasmic tail of the protein interacts with signaling molecules (such as catenin, plakoglobin) and with cytoskeleton elements. N-cadherin, like other member of the cadherin family, is found to be involved in embryonic development, tissue formation and adhesion. In cancer, disruption in the proper regulation of the cadherin proteins can lead to invasion and metastasis. 10-12


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, ICC/IF, ICC
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC, ICC
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Species: Hu, Mu, Ma
Applications: WB
Species: Hu, Po
Applications: Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, EM, Flow, ICC/IF, IHC, IHC-Fr

Publications for N-Cadherin Antibody (NBP2-21805) (0)

There are no publications for N-Cadherin Antibody (NBP2-21805).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for N-Cadherin Antibody (NBP2-21805) (0)

There are no reviews for N-Cadherin Antibody (NBP2-21805). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP2-21805) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional N-Cadherin Products

Bioinformatics Tool for N-Cadherin Antibody (NBP2-21805)

Discover related pathways, diseases and genes to N-Cadherin Antibody (NBP2-21805). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for N-Cadherin Antibody (NBP2-21805)

Discover more about diseases related to N-Cadherin Antibody (NBP2-21805).

Pathways for N-Cadherin Antibody (NBP2-21805)

View related products by pathway.

PTMs for N-Cadherin Antibody (NBP2-21805)

Learn more about PTMs related to N-Cadherin Antibody (NBP2-21805).

Research Areas for N-Cadherin Antibody (NBP2-21805)

Find related products by research area.

Blogs on N-Cadherin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

MMP24 (Matrix metalloproteinase-24, matrix metalloproteinase-25, MT5-MMP)
MMP24 is an extracellular matrix (ECM) degradative peptidase enzyme that is a member of the large family of matrix metalloproteinases (MMP). Each MMP has a different substrate specificity, and the aberrant or derailed expression of these is strongly c...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our N-Cadherin Antibody (GC-4) and receive a gift card or discount.


Gene Symbol CDH2