N-Cadherin Antibody (GC-4)

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, Flow, ICC/IF, IHC, IHC-Fr
Clone
GC-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

N-Cadherin Antibody (GC-4) Summary

Immunogen
Affinity purified chicken heart A-CAM.
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 10-20ug/ml
  • Electron Microscopy
  • Flow Cytometry 1:50-1:250
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 10-20ug/ml
Application Notes
Immunohistochemistry: 10-20 ug/mL using rat cardiac muscle frozen sections, Western blot: 10-20 ug/mL using chicken cardiac muscle or COS-7 cell extracts
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

chicken, monkey, rabbit, human, mouse, rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A or G purified

Alternate Names for N-Cadherin Antibody (GC-4)

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin

Background

Clone GC-4 is also known as GB-9. Monoclonal Anti-N-Cadherin (mouse IgG1 isotype) is derived from the GC-4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with affinity purified chicken heart A-CAM. 1 The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-N-Cadherin/A-CAM is specific for the N-Cadherin. The antibody reacts with the N-terminal half of the extracellular domain of N-Cadherin. 1, 2 It recognizes a polypeptide of 135 kDa isolated from a freshly prepared extract chicken cardiac muscle by immunoblotting. This antibody can work as a neutralizing antibody that can inhibit adherens junction formation, 3 colony formation, cell adhesion, interaction between bone marrow hematopoietic cells, 4 and cytoskeleton formation. 5 Monoclonal Anti-N-Cadherin reacts with the N-Cadherin molecule from human, 4, 5 monkey, 6 rabbit, rat, 3 mouse, 7 and chicken. 1-3 The antibody may be used in various immunochemical techniques including immunohistochemistry, immunocytochemistry, 3, 6 electron microscopy, immunoblotting, 8 and FACS analysis. 4, 9 N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin was cloned from neural tissues but later on found to be expressed mainly in endothelial and muscle tissues. Like the other classical cadherin proteins (E- and P-cadherin), N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. 10-12 This protein mediates the intercellular adhesion by homophilic interaction between the extracellular domains of the cadherin proteins in neighboring cells. The cytoplasmic tail of the protein interacts with signaling molecules (such as catenin, plakoglobin) and with cytoskeleton elements. N-cadherin, like other member of the cadherin family, is found to be involved in embryonic development, tissue formation and adhesion. In cancer, disruption in the proper regulation of the cadherin proteins can lead to invasion and metastasis. 10-12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDH2
Entrez