MyD88 Inhibitor Peptide Set

Product Discontinued

MyD88 Inhibitor Peptide Set Summary

• Immunogen: DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT).
• Preparation Method: Preparation of 5 mM Stock Solutions
  PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
  MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT
  Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
  
  Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
  Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing
  *Recipe for 1X PBS:
  1. Dissolve the following in 800ml distilled H2O.
  - 8g of NaCl
  - 0.2g of KCl
  - 1.44g of Na2HPO4
  - 0.24g of KH2PO4
  2. Adjust pH to 7.5 with HCl.
  3. Adjust volume to 1L with additional distilled H2O.
  4. Sterilize by autoclaving
• Content: The MyD88 Homodimerization Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100
• Gene: MYD88

Applications/Dilutions

• Dilutions:
  • Functional (Inhibition)
• Application Notes: Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.
  
  Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands. Use in Functional inhibition reported in multiple pieces of scientific literature

Reactivity Notes

Human, Mouse, Rat, Zebrafish and Xenopus.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized white powder
• Concentration: LYOPH
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Notes

Functions as a decoy by binding to the MyD88 TIR domain.

Alternate Names for MyD88 Inhibitor Peptide Set

• MyD88
• MYD88D
• myeloid differentiation primary response gene (88)
• myeloid differentiation primary response protein MyD88

Background

MyD88 is an adapter protein that links Toll-like receptors (TLRs) and Interleukin-1 receptors (IL-1rs) with downstream signaling molecules. Homodimerization of MyD88 is a critical step in the signaling process, and allows the recruitment and activation of the kinase IRAK. MyD88 exists as a homodimer when recruited to activated TLR/IL-1Rs. The inhibitor peptide contains a sequence from the MyD88 TIR homodimerization domain (Loiarro, et al.). 1 MyD88 monomer binds to this inhibitor peptide, there by blocking MyD88 homodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYD88