MYCBP2 Antibody

Western Blot: MYCBP2 Antibody [NB100-97841] - Whole cell lysate (5, 15, 50 ug loaded) from HeLa cells probed with anti-MYCBP2 antibody at 0.04 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MYCBP2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2425 and 2475 of human protein associated with Myc using the numbering given in entry NP_055872.3 (GeneID 23077).
• Localization: Nucleus
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYCBP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
• Application Notes: IP Not recommended, use rabbit anti-PAM antibody NB100-97840.
• Theoretical MW: 510 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MYCBP2 Antibody

• DKFZp686M08244
• EC 6.3.2.-
• FLJ10106
• FLJ21646
• KIAA0916FLJ13826
• MYC binding protein 2
• Myc-binding protein 2
• Pam/highwire/rpm-1 protein
• PAMFLJ21597
• probable E3 ubiquitin-protein ligase MYCBP2
• Protein associated with Myc

Background

In addition to its possible functions as a facilitator of Myc activated transcription, PAM (protein associated with Myc) has also been shown to play a role in mTOR signaling. PAM functions as an E3 ubiquitin ligase for tuberin (TSC2), a negative regulator of the mTOR pathway. Additionally PAM has been shown to function as a guanosine exchange factor (GEF) for Rheb and to facilitate sphingosine-1-phosphate signaling in the mTOR pathway. Alternative names for PAM include Myc-binding protein 2, MYCBP2, Pam/highwire/rpm-1 protein, and KIAA0916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYCBP2
• Entrez:
  • 23077(Human)
• Uniprot:
  • O75592(Human)