Butyrylcholinesterase/BCHE Antibody [Unconjugated]

Western blot shows lysates of mouse heart tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Polyclonal Antibody (Catalog # AF9024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Butyrylcholinesterase/BCHE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Butyrylcholinesterase/BCHE Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Butyrylcholinesterase/BCHE
  His28-Leu603
  Accession # AAH99977
• Specificity: Detects mouse and rat Butyrylcholinesterase/BCHE in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Bche
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Butyrylcholinesterase/BCHE Antibody [Unconjugated]

• Acylcholine acylhydrolase
• BCHE
• Butyrylcholine esterase
• Butyrylcholinesterase
• CHE1
• CHE1cholinesterase
• Choline esterase II
• cholinesterase 1
• E1
• EC 3.1.1.8
• Pseudocholinesterase

Background

Butyrylcholinesterase (BCHE) is a major acetylcholine hydrolyzing enzyme in the circulation (1). Although it is present in significant amounts (~3 mg/L) in human plasma, no endogenous physiological substrate has been described for this enzyme. It can degrade a large number of ester-containing compounds in addition to acylcholines. Thus, it is likely to play significant pharmacological and toxicological roles. It is thought to be involved in the pathological process of Alzheimer's disease (AD) by depleting acetylcholine. In contrast to ACHE, it attenuates amyloid fibril formation in vitro (2). BCHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance acetylcholine availability (3). Its involvement in a cholinergic anti-inflammatory pathway connect BCHE and ACHE with a possible marker of low-grade systemic inflammation observed in Type-2 diabetes, obesity, hypertension, coronary heart disease, and AD (4). BCHE can exist in monomeric and multimeric forms (1).

1. Darvesh, S. et al. (2003) Nat. Rev. Neuroscience 4:131.
2. Diamant, S. et al. (2006) Proc. Natl. Acad. Sci. USA 103:8628.
3. Campbell, V. A. and Gowran, A. (2007) Br. J. Pharm. 152:655.
4. Das, U. N. (2007) Med Sci Monit. 13:RA214.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Butyrylcholinesterase/BCHE Antibody (AF9024)(1)

Publication using AF9024

• C Fredolini, S Byström, L Sanchez-Ri, M Ioannou, D Tamburro, F Pontén, RM Branca, P Nilsson, J Lehtiö, JM Schwenk Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles Sci Rep, 2019;9(1):8324. 2019 [PMID: 31171813] (ELISA Detection, Human)

Bioinformatics

• Gene Symbol: Bche
• Uniprot:
  • AAH99977()