MMP-2 Antibody (2C1) - (Pro and Active)

Images

 
Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - MMP2 Antibody (2C1) [NB200-113] - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by ...read more
Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) ...read more
Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GPSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Expression of Tagged Proteins

MMP-2 Antibody (2C1) - (Pro and Active) Summary

Immunogen
Activated recombinant human MMP2. [UniProt# P08253]
Specificity
This is specific for pro and active MMP-2.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MMP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • ELISA 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin 5ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
This MMP2 (2C1) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24828425)
Publications
Read Publications using
NB200-113 in the following applications:

  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Source and Preparation: MMP2 (2C1) hybridoma culture medium. The hybridoma was grown in medium supplemented with Ig-depleted fetal bovine serum (serum passed through a Protein G column). The monoclonal mouse antibody was purified by Protein G affinity chromatography (and contains no detectable bovine immunoglobulins).

Alternate Names for MMP-2 Antibody (2C1) - (Pro and Active)

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC 3.4.24.24
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 (matrix metalloproteinase-2; CLG4A) is a ubiquitinous metalloproteinase enzyme belonging to peptidase M10A family which is implicated in versatile activities including vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. Besides degrading proteins in the extracellular matrix, MMP2 also acts on various nonmatrix proteins such as big endothelial 1, beta-type CGRP promoting vasoconstriction, KISS etc. MMP2 plays a key role in myocardial cell death pathways and also contributes to myocardial oxidative stress through regulation of GSK3-beta activity. PKC facilitates MDM2 phosphorylation which is crucial to its enzymatic activity. MMP2 propeptide is processed by MMP14/MT-MMP1 and MMP16/MT-MMP3, and a C-terminal autocatalytic cleavage generates PEX chain which exerts anti-angiogenic as well as anti-cancer properties alongwith inhibiting cell migration or cell adhesion to FGF2/vitronectin. MMP2's isoform 2 facilitates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of pro-inflammatory NFkB, NFAT and IRF transcriptional pathways. MMP2 is implicated in several pathologies including metastasis of cancer and defects in MMP2 have been linked to Torg-Winchester syndrome (TWS) which is also called as multicentric osteolysis nodulosis and arthropathy (MONA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-2 Antibody (NB200-113)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-2 Antibody (NB200-113). (Showing 1 - 1 of 1 FAQs).

  1. Could you guarantee NB200-113 and NB200-114 both detect human pro-form and active form MMP2 ?
    • Yes, both of your mentioned antibodies (i.e. NB200-113 and NB200-114) detects the pro (approximately 70kD) as well as the activated forms (approximately 62kD) of MMP-2 in Western Blot. In fact, the validation image on the datasheet of #NB200-114 shows a very nice blot where these two forms are very evident and this antibody has been cited in 8 peer reviewed publications.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-113AF405
Alexa Fluor 488 Labeled NB200-113AF488
Alexa Fluor 647 Labeled NB200-113AF647
Alexa Fluor 700 Labeled NB200-113AF700
Biotin Labeled NB200-113B
DyLight 350 Labeled NB200-113UV
DyLight 405 Labeled NB200-113V
DyLight 488 Labeled NB200-113G
DyLight 550 Labeled NB200-113R
DyLight 650 Labeled NB200-113C
DyLight 680 Labeled NB200-113FR
DyLight 755 Labeled NB200-113IR
FITC Labeled NB200-113F
HRP Labeled NB200-113H

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Bioinformatics Tool for MMP-2 Antibody (NB200-113)

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Research Areas for MMP-2 Antibody (NB200-113)

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Blogs on MMP-2.

MMP-2: More Than a Cancer Marker
Matrix metalloproteinases (MMP) are a family of endopeptidases involved in the breakdown of extracellular matrix (ECM) during both normal physiological and disease processes. MMP-2 is a zinc-dependent family member that selectively cleaves collagen...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol MMP2
Entrez