MMP-2 Antibody (2C1) - (Pro and Active) - BSA Free

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue)Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.

Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MMP-2 Antibody (2C1) - (Pro and Active) - BSA Free Summary

• Immunogen: Activated recombinant human MMP2. [UniProt# P08253]
• Specificity: This is specific for pro and active MMP-2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin 5ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2-5 ug/ml
• Application Notes: This MMP2 (2C1) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 24828425). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30681163).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Notes

Source and Preparation: MMP2 (2C1) hybridoma culture medium. The hybridoma was grown in medium supplemented with Ig-depleted fetal bovine serum (serum passed through a Protein G column). The monoclonal mouse antibody was purified by Protein G affinity chromatography (and contains no detectable bovine immunoglobulins).

Alternate Names for MMP-2 Antibody (2C1) - (Pro and Active) - BSA Free

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 (matrix metalloproteinase-2; CLG4A) is a ubiquitinous metalloproteinase enzyme belonging to peptidase M10A family which is implicated in versatile activities including vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. Besides degrading proteins in the extracellular matrix, MMP2 also acts on various nonmatrix proteins such as big endothelial 1, beta-type CGRP promoting vasoconstriction, KISS etc. MMP2 plays a key role in myocardial cell death pathways and also contributes to myocardial oxidative stress through regulation of GSK3-beta activity. PKC facilitates MDM2 phosphorylation which is crucial to its enzymatic activity. MMP2 propeptide is processed by MMP14/MT-MMP1 and MMP16/MT-MMP3, and a C-terminal autocatalytic cleavage generates PEX chain which exerts anti-angiogenic as well as anti-cancer properties alongwith inhibiting cell migration or cell adhesion to FGF2/vitronectin. MMP2's isoform 2 facilitates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of pro-inflammatory NFkB, NFAT and IRF transcriptional pathways. MMP2 is implicated in several pathologies including metastasis of cancer and defects in MMP2 have been linked to Torg-Winchester syndrome (TWS) which is also called as multicentric osteolysis nodulosis and arthropathy (MONA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-2 Antibody (NB200-113)(4)

Publications using NB200-113

• Schilling BK, Schusterman MA, Kim DY et al. Adipose-Derived Stem Cells Delay Muscle Atrophy after Peripheral Nerve Injury in the Rodent Model. Muscle Nerve Jan 25 2019 [PMID: 30681163] (ICC/IF, Rat)
• Scheede-Bergdahl C, Bergdahl A, Schjerling P et al. Exercise-induced regulation of matrix metalloproteinases in the skeletal muscle of subjects with type 2 diabetes Diab Vasc Dis Res. 2014 Jun 04 [PMID: 24903024] (WB, Human)
  
  Details:
  MMP2 antibody used for WB (1:1000 dilution) on human skeletal muscle lysates from type 2 diabetes patients (T2DM / NIDDM) and control subjects (Full Text has detailed method on tissue lysis). Antibody detected the 72 kDa band of pro-MMP-2 and 62 kDa band of active-MMP-2 (Figure 3)
• Xu H, Zhang Y, Sun H et al. Effects of Acupuncture at GV20 and ST36 on the Expression of Matrix Metalloproteinase 2, Aquaporin 4, and Aquaporin 9 in Rats Subjected to Cerebral Ischemia/Reperfusion Injury. PLoS ONE. 2014 May 15 [PMID: 24828425] (WB, IHC-P, Rat)
• Rork TH, Hadzimichalis NM, Kappil MA, Merrill GF. Acetaminophen attenuates peroxynitrite-activated matrix metalloproteinase-2-mediated troponin I cleavage in the isolated guinea pig myocardium. J Mol Cell Cardiol 40(4):553-61. 2006 Apr. [PMID: 16530785] (WB, Guinea Pig)

FAQs for MMP-2 Antibody (NB200-113). (Showing 1 - 1 of 1 FAQs).

1. Could you guarantee NB200-113 and NB200-114 both detect human pro-form and active form MMP2 ?
   • Yes, both of your mentioned antibodies (i.e. NB200-113 and NB200-114) detects the pro (approximately 70kD) as well as the activated forms (approximately 62kD) of MMP-2 in Western Blot. In fact, the validation image on the datasheet of #NB200-114 shows a very nice blot where these two forms are very evident and this antibody has been cited in 8 peer reviewed publications.

Bioinformatics

• Gene Symbol: MMP2