Human TIMP-2 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human TIMP-2 Quantikine ELISA Kit Summary

Background
The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-2 levels in cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human TIMP-2, and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant... Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-2.
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Specificity
Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TIMP2

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DTM200.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-2 Quantikine ELISA Kit

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIMP-2 (DTM200)(32)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(30)
Mouse
(2)
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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using DTM200 Applications Species
JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death & Disease, 2022;13(6):527. 2022 [PMID: 35661105] (Human) Human
X Tao, C Chen, W Luo, J Zhou, J Tian, X Yang, FF Hou Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis Bmc Nephrology, 2021;22(1):415. 2021 [PMID: 34906098] (Human) Human
K Moriya, N Nishimura, T Namisaki, H Takaya, Y Sawada, H Kawaratani, K Kaji, N Shimozato, S Sato, M Furukawa, A Douhara, T Akahane, A Mitoro, J Yamao, H Yoshiji Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis Journal of Clinical Medicine, 2021;10(11):. 2021 [PMID: 34199421] (Human) Human
K Esmeijer, A Schoe, LR Ruhaak, EK Hoogeveen, D Soonawala, FPHTM Romijn, MR Shirzada, JT van Dissel, CM Cobbaert, JW de Fijter The predictive value of TIMP-2 and IGFBP7 for kidney failure and 30-day mortality after elective cardiac surgery Scientific Reports, 2021;11(1):1071. 2021 [PMID: 33441876] (Human) Human
A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report Int J Mol Sci, 2020;21(22):. 2020 [PMID: 33218057] (Human) Human
G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier Int J Mol Sci, 2020;21(11):. 2020 [PMID: 32481745] (Mouse) Mouse
AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma Oncol Lett, 2020;19(4):3011-3020. 2020 [PMID: 32218858] (Human) Human
A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls Biomed Res Int, 2019;2019(0):7420345. 2019 [PMID: 31781639] (Human) Human
DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1 Front Neurosci, 2019;13(0):836. 2019 [PMID: 31474817] (Human) Human
SEI Dekker, LR Ruhaak, FPHTM Romijn, E Meijer, CM Cobbaert, JW de Fijter, D Soonawala, Urinary Tissue Inhibitor of�Metalloproteinases-2 and Insulin-Like Growth Factor-Binding Protein 7 Do Not Correlate With Disease Severity in ADPKD Patients Kidney Int Rep, 2019;4(6):833-841. 2019 [PMID: 31194166] (Human) Human
Show All 32 Publications.

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Bioinformatics Tool for TIMP-2 (DTM200)

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Blogs on TIMP-2.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol TIMP2