Human TIMP-2 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human TIMP-2 Quantikine ELISA Kit Summary

Background
The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-2 levels in cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human TIMP-2, and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant... Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-2.
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Specificity
Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TIMP2

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DTM200.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-2 Quantikine ELISA Kit

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIMP-2 (DTM200)(24)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(23)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using DTM200 Applications Species
A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls Biomed Res Int, 2019;2019(0):7420345. 2019 [PMID: 31781639] (Human) Human
DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1 Front Neurosci, 2019;13(0):836. 2019 [PMID: 31474817] (Human) Human
R Schmitt, A Tscheuschl, P Laschinski, X Uffelmann, P Discher, J Fuchs, M Kreibich, R Peyronnet, FA Kari A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2 PLoS ONE, 2019;14(2):e0212859. 2019 [PMID: 30794673] (Human) Human
M ?ukaszewic, M Gryko, S P?czek, M Szmitkowsk, B K?dra, B Mroczko Matrix metalloproteinase 2 (MMP-2) and its tissue inhibitor 2 (TIMP-2) in pancreatic cancer (PC) Oncotarget, 2019;10(3):395-403. 2019 [PMID: 30719232] (Human) Human
MY Ahn, BJ Kim, HJ Kim, JM Jin, HJ Yoon, JS Hwang, KK Park Anti-cancer effect of dung beetle glycosaminoglycans on melanoma BMC Cancer, 2019;19(1):9. 2019 [PMID: 30611221] (Human) Human
CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 2018;7(11):. 2018 [PMID: 30413105] (Human) Human
JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray Human umbilical cord plasma proteins revitalize hippocampal function in aged mice Nature, 2017;544(7651):488-492. 2017 [PMID: 28424512] (Human) Human
ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model PLoS ONE, 2017;12(2):e0171191. 2017 [PMID: 28192449] (Human) Human
Braicu E, Gasimli K, Richter R, Nassir M, Kummel S, Blohmer J, Yalcinkaya I, Chekerov R, Ignat I, Ionescu A, Mentze M, Fotopoulou C, Pop C, Lichtenegger W, Sehouli J Role of serum VEGFA, TIMP2, MMP2 and MMP9 in monitoring response to adjuvant radiochemotherapy in patients with primary cervical cancer--results of a companion protocol of the randomized NOGGO-AGO phase III clinical trial. Anticancer Res, 2014;34(1):385-91. 2014 [PMID: 24403492] (Human) Human
Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy. Cytokine, 2013;61(3):755-8. 2013 [PMID: 23415672] (Human) Human
Show All 24 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TIMP-2 (DTM200). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DTM200

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Bioinformatics Tool for TIMP-2 (DTM200)

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Blogs on TIMP-2.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol TIMP2