Human TIMP-2 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TIMP-2 Quantikine ELISA Kit Summary

• Background: The Quantikine Human TIMP-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TIMP-2 levels in cell culture supernates, serum, plasma, saliva, and urine. It contains recombinant human TIMP-2 and antibodies raised against the recombinant protein. Natural human TIMP-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quanti...kine kit standards, indicating that this kit can be used to determine relative levels of human TIMP-2.
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• Specificity: Natural and recombinant human TIMP-2. This assay detects approximately 50% of recombinant human TIMP-2 when complexed with recombinant human active MMP-9 in a 1:1 molar ratio.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TIMP2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-2 Quantikine ELISA Kit

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIMP-2 (DTM200)(38)

Publications using DTM200

• Habib, EM;Hasan, AM;Mohammed, SAA;Othman, AAA;Elgamal, R;Sherief, DE; Prognostic and diagnostic value of PVR gene and protein levels, serum amylase, and urinary IGFBP-7 and TIMP-2 biomarkers in multiple myeloma BMC cancer 2025-05-14 [PMID: 40369504] (Human)
• Baek, S;Park, I;Kim, S;Um, YW;Kim, HE;Lee, K;Lee, JH;Jo, YH; Urinary biomarkers for diagnosing acute kidney injury in sepsis in the emergency department Heliyon 2025-01-15 [PMID: 39811377] (Human)
• de Rooij, ENM;Hoogeveen, EK;Romijn, FPHTM;van der Kooy, SW;Veighey, KV;Dekker, FW;van Kooten, C;Cobbaert, CM;de Fijter, JW; Urinary beta-2 microglobulin increases whereas TIMP-2 and IGFBP7 decline after unilateral nephrectomy in healthy kidney donors Scientific reports 2024-06-05 [PMID: 38839764] (Human)
• I Tsomidis, G Notas, C Xidakis, A Voumvourak, DN Samonakis, M Koulentaki, E Kouroumali Enzymes of Fibrosis in Chronic Liver Disease Biomedicines, 2022-12-08;10(12):. 2022-12-08 [PMID: 36551935] (Human)
• N Nasiri-Ans, E Spilioti, I Kyrou, V Kalotychou, A Chatzigeor, D Sanoudou, K Dahlman-Wr, HS Randeva, AG Papavassil, P Moutsatsou, E Kassi Estrogen Receptor Subtypes Elicit a Distinct Gene Expression Profile of Endothelial-Derived Factors Implicated in Atherosclerotic Plaque Vulnerability International Journal of Molecular Sciences, 2022-09-19;23(18):. 2022-09-19 [PMID: 36142876] (Human)
• TE Peterson, C Landon, SA Haberlen, F Bhondoekha, MW Plankey, FJ Palella, DA Piggott, JB Margolick, TT Brown, WS Post, KC Wu Circulating biomarker correlates of left atrial size and myocardial extracellular volume fraction among persons living with and without HIV BMC cardiovascular disorders, 2022-09-03;22(1):393. 2022-09-03 [PMID: 36057773] (Human)
• JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death & Disease, 2022-06-06;13(6):527. 2022-06-06 [PMID: 35661105] (Human)
• X Tao, C Chen, W Luo, J Zhou, J Tian, X Yang, FF Hou Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis Bmc Nephrology, 2021-12-15;22(1):415. 2021-12-15 [PMID: 34906098] (Human)
• K Moriya, N Nishimura, T Namisaki, H Takaya, Y Sawada, H Kawaratani, K Kaji, N Shimozato, S Sato, M Furukawa, A Douhara, T Akahane, A Mitoro, J Yamao, H Yoshiji Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis Journal of Clinical Medicine, 2021-06-02;10(11):. 2021-06-02 [PMID: 34199421] (Human)
• K Esmeijer, A Schoe, LR Ruhaak, EK Hoogeveen, D Soonawala, FPHTM Romijn, MR Shirzada, JT van Dissel, CM Cobbaert, JW de Fijter The predictive value of TIMP-2 and IGFBP7 for kidney failure and 30-day mortality after elective cardiac surgery Scientific Reports, 2021-01-13;11(1):1071. 2021-01-13 [PMID: 33441876] (Human)
• A Waszczykow, M Podgórski, M Waszczykow, Z Gerlicz-Ko, P Jurowski Matrix Metalloproteinases MMP-2 and MMP-9, Their Inhibitors TIMP-1 and TIMP-2, Vascular Endothelial Growth Factor and sVEGFR-2 as Predictive Markers of Ischemic Retinopathy in Patients with Systemic Sclerosis-Case Series Report Int J Mol Sci, 2020-11-18;21(22):. 2020-11-18 [PMID: 33218057] (Human)
• G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier Int J Mol Sci, 2020-05-28;21(11):. 2020-05-28 [PMID: 32481745] (Mouse)
• AE Stanciu, A Zamfir-Chi, MM Stanciu, A Pantea-Sto, C Nitipir, DC Gheorghe Serum melatonin is inversely associated with matrix metalloproteinase-9 in oral squamous cell carcinoma Oncol Lett, 2020-02-13;19(4):3011-3020. 2020-02-13 [PMID: 32218858] (Human)
• A Kobus, J Bagi?ska, J ?api?ska-A, S ?awicki, A Kierklo Levels of Selected Matrix Metalloproteinases, Their Inhibitors in Saliva, and Oral Status in Juvenile Idiopathic Arthritis Patients vs. Healthy Controls Biomed Res Int, 2019-10-28;2019(0):7420345. 2019-10-28 [PMID: 31781639] (Human)
• DP Zhang, YF Peng, HL Zhang, JG Ma, M Zhao, S Yin, TT Wei Basilar Artery Tortuosity Is Associated With White Matter Hyperintensities by TIMP-1 Front Neurosci, 2019-08-14;13(0):836. 2019-08-14 [PMID: 31474817] (Human)
• SEI Dekker, LR Ruhaak, FPHTM Romijn, E Meijer, CM Cobbaert, JW de Fijter, D Soonawala Urinary Tissue Inhibitor of�Metalloproteinases-2 and Insulin-Like Growth Factor-Binding Protein 7 Do Not Correlate With Disease Severity in ADPKD Patients Kidney Int Rep, 2019-03-22;4(6):833-841. 2019-03-22 [PMID: 31194166] (Human)
• R Schmitt, A Tscheuschl, P Laschinski, X Uffelmann, P Discher, J Fuchs, M Kreibich, R Peyronnet, FA Kari A potential key mechanism in ascending aortic aneurysm development: Detection of a linear relationship between MMP-14/TIMP-2 ratio and active MMP-2 PLoS ONE, 2019-02-22;14(2):e0212859. 2019-02-22 [PMID: 30794673] (Human)
• M ?ukaszewic, M Gryko, S P?czek, M Szmitkowsk, B K?dra, B Mroczko Matrix metalloproteinase 2 (MMP-2) and its tissue inhibitor 2 (TIMP-2) in pancreatic cancer (PC) Oncotarget, 2019-01-08;10(3):395-403. 2019-01-08 [PMID: 30719232] (Human)
• MY Ahn, BJ Kim, HJ Kim, JM Jin, HJ Yoon, JS Hwang, KK Park Anti-cancer effect of dung beetle glycosaminoglycans on melanoma BMC Cancer, 2019-01-05;19(1):9. 2019-01-05 [PMID: 30611221] (Human)
• CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 2018-11-08;7(11):. 2018-11-08 [PMID: 30413105] (Human)
• JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray Human umbilical cord plasma proteins revitalize hippocampal function in aged mice Nature, 2017-04-19;544(7651):488-492. 2017-04-19 [PMID: 28424512] (Human)
• ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model PLoS ONE, 2017-02-13;12(2):e0171191. 2017-02-13 [PMID: 28192449] (Human)
• Braicu E, Gasimli K, Richter R, Nassir M, Kummel S, Blohmer J, Yalcinkaya I, Chekerov R, Ignat I, Ionescu A, Mentze M, Fotopoulou C, Pop C, Lichtenegger W, Sehouli J Role of serum VEGFA, TIMP2, MMP2 and MMP9 in monitoring response to adjuvant radiochemotherapy in patients with primary cervical cancer--results of a companion protocol of the randomized NOGGO-AGO phase III clinical trial. Anticancer Res, 2014-01-01;34(1):385-91. 2014-01-01 [PMID: 24403492] (Human)
• Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy. Cytokine, 2013-02-14;61(3):755-8. 2013-02-14 [PMID: 23415672] (Human)
• Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence. J Neuroinflammation, 2011-09-09;8(0):115. 2011-09-09 [PMID: 21906275] (Mouse)
• Jonasson S, Lundberg A, Kalvegren H, Bergstrom I, Szymanowski A, Jonasson L Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris. PLoS ONE, 2011-04-29;6(4):e19340. 2011-04-29 [PMID: 21559401] (Human)
• Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. Eur. J. Haematol., 2009-11-17;84(3):239-51. 2009-11-17 [PMID: 19922462] (Human)
• Sesso R, Franco MC Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood. Am. J. Hypertens., 2009-11-05;23(1):6-11. 2009-11-05 [PMID: 19893495] (Human)
• O'Kane CM, McKeown SW, Perkins GD, Bassford CR, Gao F, Thickett DR, McAuley DF Salbutamol up-regulates matrix metalloproteinase-9 in the alveolar space in the acute respiratory distress syndrome. Crit. Care Med., 2009-07-01;37(7):2242-9. 2009-07-01 [PMID: 19487934] (Human)
• Glass-Marmor L, Paperna T, Galboiz Y, Miller A Immunomodulation by chronobiologically-based glucocorticoids treatment for multiple sclerosis relapses. J. Neuroimmunol., 2009-03-28;210(1):124-7. 2009-03-28 [PMID: 19329193] (Human)
• Kapsoritakis AN, Kapsoritaki AI, Davidi IP, Lotis VD, Manolakis AC, Mylonis PI, Theodoridou AT, Germenis AE, Potamianos SP Imbalance of tissue inhibitors of metalloproteinases (TIMP) - 1 and - 4 serum levels, in patients with inflammatory bowel disease. BMC Gastroenterol, 2008-11-26;8(1):55. 2008-11-26 [PMID: 19036126] (Human)
• McKeown S, Richter AG, O'Kane C, O'Kane C, McAuley DF, Thickett DR MMP expression and abnormal lung permeability are important determinants of outcome in IPF. Eur. Respir. J., 2008-10-01;33(1):77-84. 2008-10-01 [PMID: 18829682] (Human)
• Glader P, Eldh B, Bozinovski S, Andelid K, Sjostrand M, Malmhall C, Anderson GP, Riise GC, Qvarfordt I, Linden A Impact of acute exposure to tobacco smoke on gelatinases in the bronchoalveolar space. Eur. Respir. J., 2008-04-16;32(3):644-50. 2008-04-16 [PMID: 18417512] (Human)
• Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis? Chest, 2007-12-10;133(5):1101-6. 2007-12-10 [PMID: 18071010] (Human)
• Thrailkill KM, Bunn RC, Moreau CS, Cockrell GE, Simpson PM, Coleman HN, Frindik JP, Kemp SF, Fowlkes JL Matrix metalloproteinase-2 dysregulation in type 1 diabetes. Diabetes Care, 2007-06-11;30(9):2321-6. 2007-06-11 [PMID: 17563344] (Human)
• Bodet C, Andrian E, Tanabe S, Grenier D Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction. J. Cell. Physiol., 2007-07-01;212(1):189-94. 2007-07-01 [PMID: 17299802] (Human)
• Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury. Thromb. Haemost., 2004-03-01;91(3):506-13. 2004-03-01 [PMID: 14983226] (Human)
• Ulrich D, Hrynyschyn K, Pallua N Matrix metalloproteinases and tissue inhibitors of metalloproteinases in sera and tissue of patients with Dupuytren's disease. Plast. Reconstr. Surg., 2003-10-01;112(5):1279-86. 2003-10-01 [PMID: 14504511] (Human)

FAQs for TIMP-2 (DTM200). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: TIMP2