MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202. Image from the standard format of this antibody.

Immunohistochemistry: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - Upper image: MMP-2 FITC (green), DAPI (blue); Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review. Image from the standard format of this antibody.

Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review. Image from the standard format of this antibody.

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunohistochemistry: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) an

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free [NBP2-80853] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) an

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free Summary

• Immunogen: Activated recombinant human MMP2. [UniProt# P08253]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 30681163)
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin 5ug/ml. Use reported in scientific literature (PMID 24828425)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2-5 ug/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MMP-2 Antibody (2C1) - (Pro and Active) - Azide and BSA Free

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 (matrix metalloproteinase-2; CLG4A) is a ubiquitinous metalloproteinase enzyme belonging to peptidase M10A family which is implicated in versatile activities including vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. Besides degrading proteins in the extracellular matrix, MMP2 also acts on various nonmatrix proteins such as big endothelial 1, beta-type CGRP promoting vasoconstriction, KISS etc. MMP2 plays a key role in myocardial cell death pathways and also contributes to myocardial oxidative stress through regulation of GSK3-beta activity. PKC facilitates MDM2 phosphorylation which is crucial to its enzymatic activity. MMP2 propeptide is processed by MMP14/MT-MMP1 and MMP16/MT-MMP3, and a C-terminal autocatalytic cleavage generates PEX chain which exerts anti-angiogenic as well as anti-cancer properties alongwith inhibiting cell migration or cell adhesion to FGF2/vitronectin. MMP2's isoform 2 facilitates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of pro-inflammatory NFkB, NFAT and IRF transcriptional pathways. MMP2 is implicated in several pathologies including metastasis of cancer and defects in MMP2 have been linked to Torg-Winchester syndrome (TWS) which is also called as multicentric osteolysis nodulosis and arthropathy (MONA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP2